Anti-NMT1/NMT 抗体 (ab186123)
Key features and details
- Rabbit polyclonal to NMT1/NMT
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-NMT1/NMT antibody
NMT1/NMT 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NMT1/NMT -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
-
免疫原
Synthetic peptide within Human NMT1/NMT aa 50-100. The exact sequence is proprietary.
Database link: P30419 -
ポジティブ･コントロール
- HeLa, 293T, Jurkat, TCMK-1 and NIH3T3 whole cell lysates.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186123の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 57 kDa.
|
|
| IP |
Use a concentration of 2 - 10 µg/ml.
|
| 特記事項 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 57 kDa. |
|
IP
Use a concentration of 2 - 10 µg/ml. |
ターゲット情報
-
機能
Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins. -
組織特異性
Heart, gut, kidney, liver and placenta. -
配列類似性
Belongs to the NMT family. -
細胞内局在
Cytoplasm. - Information by UniProt
-
参照データベース
- Entrez Gene: 419966 Chicken
- Entrez Gene: 454744 Chimpanzee
- Entrez Gene: 281351 Cow
- Entrez Gene: 480494 Dog
- Entrez Gene: 101143947 Gorilla
- Entrez Gene: 100719854 Guinea pig
- Entrez Gene: 100050277 Horse
- Entrez Gene: 4836 Human
see all -
別名
- Alternative, short form NMT S antibody
- EC 2.3.1.97 antibody
- Glycylpeptide N tetradecanoyltransferase 1 antibody
see all
画像
-
Western blot - Anti-NMT1/NMT antibody (ab186123)All lanes : Anti-NMT1/NMT antibody (ab186123) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 57 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-NMT1/NMT antibody (ab186123)Lane 1: ab186123 at 1 µg/ml staining NMT1/NMT in 293T whole cell lysate immunoprecipitated using ab186123 at 6 µg/reaction (0.5 or 1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab186123 は 2 報の論文で使用されています。
- Jia M et al. Myristic acid as a checkpoint to regulate STING-dependent autophagy and interferon responses by promoting N-myristoylation. Nat Commun 14:660 (2023). PubMed: 36750575
- Zhu G et al. N-Myristoylation by NMT1 Is POTEE-Dependent to Stimulate Liver Tumorigenesis via Differentially Regulating Ubiquitination of Targets. Front Oncol 11:681366 (2021). PubMed: 34136404