Anti-NMDAR2B (phospho Y1252) 抗体 (ab18532)
Key features and details
- Rabbit polyclonal to NMDAR2B (phospho Y1252)
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-NMDAR2B (phospho Y1252) antibody
NMDAR2B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NMDAR2B (phospho Y1252) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab18532の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 166 kDa (predicted molecular weight: 166 kDa).
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 166 kDa (predicted molecular weight: 166 kDa). |
ターゲット情報
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機能
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. -
組織特異性
Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia. -
配列類似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily. -
細胞内局在
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2904 Human
- Entrez Gene: 14812 Mouse
- Entrez Gene: 24410 Rat
- Omim: 138252 Human
- SwissProt: Q13224 Human
- SwissProt: Q01097 Mouse
- SwissProt: Q00960 Rat
- Unigene: 654430 Human
see all -
別名
- AW490526 antibody
- EIEE27 antibody
- Glutamate [NMDA] receptor subunit epsilon 2 antibody
see all
画像
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Western blot - Anti-NMDAR2B (phospho Y1252) antibody (ab18532)All lanes : ab18532 at 1 mg/ml
Lane 1 : Mouse Brain Tissue Lysate
Lane 2 : Mouse Fore-brain Tissue Lysate
Lane 3 : Mouse Cortex Tissue Lysate
Lane 4 : Mouse Cerebellum Tissue Lysate
Lane 5 : Mouse Hippo-campus Tissue Lysate
Lane 6 : Human Brain Tissue Lysate
Lane 7 : Human Cortex Tissue Lysate
Lane 8 : Human Cerebellum Tissue Lysate
Lane 9 : Human Hippo-campus Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 166 kDa
Observed band size: 166 kDa
Additional bands at: 18 kDa, 45 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
Western blot - Anti-NMDAR2B (phospho Y1252) antibody (ab18532)This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United KingdomLanes 1-3 : Anti-NMDAR2B (phospho Y1252) antibody (ab18532) at 3 µg/ml
Lane 4 : NR2B antibody (positive control)
Lane 1 : mouse cortical extract with Mouse NMDAR2B (unmodified ) peptide (ab19637) at 2 µg/ml
Lane 2 : mouse cortical extract with Mouse NMDAR2B (phospho Y1252) peptide (ab19636) at 2 µg/ml
Lanes 3-4 : mouse cortical extract
Lysates/proteins at 20 µg per lane.
Predicted band size: 166 kDa
Observed band size: 166 kDa
Additional bands at: 115 kDa. We are unsure as to the identity of these extra bands.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab18532 は 4 報の論文で使用されています。
- Reyes-Méndez ME et al. Developmental light deprivation transiently reduces the expression of vGluT2 and GluN2B in the rat ventral suprachiasmatic nucleus. Synapse 77:e22250 (2023). PubMed: 36085433
- Herrera-Zamora JM et al. Development-Dependent Changes in the NR2 Subtype of the N-Methyl-D-Aspartate Receptor in the Suprachiasmatic Nucleus of the Rat. J Biol Rhythms 34:39-50 (2019). PubMed: 30654688
- Sánchez-Blázquez P et al. Sigma 1 Receptor Antagonists Inhibit Manic-Like Behaviors in Two Congenital Strains of Mice. Int J Neuropsychopharmacol 21:938-948 (2018). PubMed: 29860313
- Delint-Ramirez I et al. In vivo composition of NMDA receptor signaling complexes differs between membrane subdomains and is modulated by PSD-95 and PSD-93. J Neurosci 30:8162-70 (2010). PubMed: 20554866