Anti-NMDAR2B 抗体 [S59] (ab93610)
![Immunocytochemistry/ Immunofluorescence - Anti-NMDAR2B antibody [S59] (ab93610)](https://www.abcam.co.jp/ps/products/93/ab93610/Images/ab93610-511872-anti-nmdar2b-antibody-n59-36-immunocytochemistry-sh-sy5y-human.jpg "Immunocytochemistry/ Immunofluorescence - Anti-NMDAR2B antibody [S59] (ab93610)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [S59] to NMDAR2B
- Suitable for: Flow Cyt, ICC/IF, IHC-P, IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-NMDAR2B antibody [S59]
NMDAR2B 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [S59] to NMDAR2B -
由来種
Mouse -
特異性
No cross-reactivity with NMDAR2A. -
アプリケーション
適用あり: Flow Cyt, ICC/IF, IHC-P, IP, WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Dog
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免疫原
Recombinant fragment within Rat NMDAR2B aa 1-300 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q00960 -
ポジティブ･コントロール
- Flow Cyt: SH-SY5Y cells. WB: Rat brain lysate. IHC-P : mouse backskin. ICC/IF: SH-SY5Y cells.
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特記事項
The clone number has been updated from S59-36 to N59/36, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
S59 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab93610の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | (1) |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
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| WB |
Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 166 kDa (predicted molecular weight: 166 kDa).
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| 特記事項 |
|---|
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Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
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WB
Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 166 kDa (predicted molecular weight: 166 kDa). |
ターゲット情報
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機能
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. -
組織特異性
Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia. -
配列類似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily. -
細胞内局在
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 494009 Dog
- Entrez Gene: 2904 Human
- Entrez Gene: 14812 Mouse
- Entrez Gene: 24410 Rat
- Omim: 138252 Human
- SwissProt: Q5R1P3 Dog
- SwissProt: Q13224 Human
- SwissProt: Q01097 Mouse
see all -
別名
- AW490526 antibody
- EIEE27 antibody
- Glutamate [NMDA] receptor subunit epsilon 2 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-NMDAR2B antibody [S59] (ab93610)
Immunocytochemistry/Immunofluorescence analysisi of 4% PFA fixed Human Neuroblastoma cells (SH-SY5Y) using ab93610 at 1/50 dilution. The section was incubated with ab93610 overnight at 4°C with slow rocking followed by AlexaFluor 488 secindary antibody at 1/100 dilution for 1 hour at RT (Green). Hoechst was used as nuclear stain (A) and Phalloidin-iFluor 647 (red) was used as actin counterstaining (B).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [S59] (ab93610)](https://www.abcam.co.jp/ps/products/93/ab93610/Images/ab93610-511871-anti-nmdar2b-antibody-n59-36-immunohistochemistry-backskin-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [S59] (ab93610)Immunohistochemistry analysis of Bouin’s Fixed and paraffin-embedded Mouse backskin tissue using ab93610 at 1/100 dilutioon. The section was incubated with ab93610 for 1 hour at RT followed by FITC Goat Anti-Mouse secondary antibody at 1/50 dilution for 1 hour at RT (green). Positive Filaggrin-like staining and dermal staining observed with mouse backskin tissue.
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![Western blot - Anti-NMDAR2B antibody [S59] (ab93610)](https://www.abcam.co.jp/ps/products/93/ab93610/Images/ab93610-316181-anti-nmdar2b-antibody-n59-36-western-blot.jpg)
Western blot - Anti-NMDAR2B antibody [S59] (ab93610)All lanes : Anti-NMDAR2B antibody [S59] (ab93610) at 1/250 dilution
Lane 1 : Induced HEK-T lysates
Lane 2 : Non-Induced HEK-T lysates
Lane 3 : P23X HEK-T negative control lysates
Predicted band size: 166 kDa -
![Flow Cytometry - Anti-NMDAR2B antibody [S59] (ab93610)](https://www.abcam.co.jp/ps/products/93/ab93610/Images/ab93610-108651-anti-nmdar2b-antibody-n59-36-flow-cytometry.jpg)
Flow Cytometry - Anti-NMDAR2B antibody [S59] (ab93610)Overlay histogram showing SH-SY5Y cells stained with ab93610 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93610, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed SH-SY5Y cells used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback -
![Western blot - Anti-NMDAR2B antibody [S59] (ab93610)](https://www.abcam.co.jp/ps/products/93/ab93610/Images/ab93610-316182-anti-nmdar2b-antibody-n59-36-western-blot.jpg)
Western blot - Anti-NMDAR2B antibody [S59] (ab93610)Anti-NMDAR2B antibody [S59] (ab93610) at 1/1000 dilution + Rat brain membrane lysate at 15 µg
Secondary
Sheep Anti-Mouse IgG: HRP for 1 hour at RT
Predicted band size: 166 kDaBlocking buffer: 1.5% BSA for 30 minutes at RT.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (26)
ab93610 は 26 報の論文で使用されています。
- Wang H et al. The dose-dependent effect of 1.5-GHz microwave exposure on spatial memory and the NMDAR pathway in Wistar rats. Environ Sci Pollut Res Int 30:37427-37439 (2023). PubMed: 36574118
- Serwach K et al. STIM2 regulates NMDA receptor endocytosis that is induced by short-term NMDA receptor overactivation in cortical neurons. Cell Mol Life Sci 80:368 (2023). PubMed: 37989792
- Seifried L et al. Cognitive Flexibility in Mice: Effects of Puberty and Role of NMDA Receptor Subunits. Cells 12:N/A (2023). PubMed: 37174612
- Hashimoto Y et al. Brain Dp140 alters glutamatergic transmission and social behaviour in the mdx52 mouse model of Duchenne muscular dystrophy. Prog Neurobiol 216:102288 (2022). PubMed: 35654209
- Zhang X et al. Rejuvenation of the aged brain immune cell landscape in mice through p16-positive senescent cell clearance. Nat Commun 13:5671 (2022). PubMed: 36167854