Anti-NMDAR2B 抗体 [NR2B] (ab28373)
Key features and details
- Mouse monoclonal [NR2B] to NMDAR2B
- Suitable for: WB, IHC-P, ICC, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-NMDAR2B antibody [NR2B]
NMDAR2B 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [NR2B] to NMDAR2B -
由来種
Mouse -
特異性
Detects the N-methyl-D-aspartate (NMDA) receptor type 2B. This antibody has been tested in mouse and rat brain in western blotting. It has not been tested in human for Western blotting - only for ICC on transfected HEK cells. -
アプリケーション
適用あり: WB, IHC-P, ICC, Flow Cytmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Rat NMDAR2B aa 934-1457.
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ポジティブ・コントロール
- WB: Mouse brain tissue ICC: transfected HEK cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Protein A Chromatography -
ポリ/モノ
モノクローナル -
クローン名
NR2B -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab28373の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
Use a concentration of 2 µg/ml. Predicted molecular weight: 166 kDa.
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IHC-P |
1/10 - 1/100.
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ICC | (1) |
Use at an assay dependent concentration.
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Flow Cyt |
Use 1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 166 kDa. |
IHC-P
1/10 - 1/100. |
ICC
Use at an assay dependent concentration. |
Flow Cyt
Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. -
組織特異性
Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia. -
配列類似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily. -
細胞内局在
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2904 Human
- Entrez Gene: 14812 Mouse
- Entrez Gene: 24410 Rat
- Omim: 138252 Human
- SwissProt: Q13224 Human
- SwissProt: Q01097 Mouse
- SwissProt: Q00960 Rat
- Unigene: 654430 Human
see all -
別名
- AW490526 antibody
- EIEE27 antibody
- Glutamate [NMDA] receptor subunit epsilon 2 antibody
see all
画像
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Anti-NMDAR2B antibody [NR2B] (ab28373) at 2 µg/ml + mouse brain tissue
Predicted band size: 166 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [NR2B] (ab28373)
ab28373 staining NMDAR2B in Human breast tissue sections (right) compared with negative control (left) by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 3% H2O2-methanol for 15 minutes at room temperature; antigen retrieval was by heat mediation in a sodium citrate buffer. Samples were incubated with primary antibody (1/20) overnight at 4°C. A HRP-conjugated secondary antibody was used for detection.
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Lanes 2-4 : Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/500 dilution
Lanes 5-7 : Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/1000 dilution
Lanes 3 & 6 : Mouse brain (50ug)
Lanes 4 & 7 : Rat brain (50ug)
Predicted band size: 166 kDa -
Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)This image is courtesy of an anonymous AbreviewAnti-NMDAR2B antibody [NR2B] (ab28373) at 1/500 dilution + Rat P14 Cortex Lysate, vesicular fraction at 5 µg
Secondary
HRP conjugated Donkey anti-mouse IgG at 1/15000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 166 kDaSamples were incubated with primary antibody for 12 hours at 4ºC.
Blocked with 5% milk for 1 hour at 22ºC.
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Overlay histogram showing SH-SY5Y cells stained with ab28373 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab28373, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (36)
ab28373 は 36 報の論文で使用されています。
- Choi JE et al. Round-window delivery of lithium chloride regenerates cochlear synapses damaged by noise-induced excitotoxic trauma via inhibition of the NMDA receptor in the rat. PLoS One 18:e0284626 (2023). PubMed: 37216352
- Han MX et al. Gao-Zi-Yao improves learning and memory function in old spontaneous hypertensive rats. BMC Complement Med Ther 22:147 (2022). PubMed: 35643519
- Zhuang ZF et al. N-Methyl D-aspartate receptor subtype 2B/Ca2+/calmodulin-dependent protein kinase II signaling in the lateral habenula regulates orofacial allodynia and anxiety-like behaviors in a mouse model of trigeminal neuralgia. Front Cell Neurosci 16:981190 (2022). PubMed: 36187288
- Belén Sanz-Martos A et al. Butyric Acid Precursor Tributyrin Modulates Hippocampal Synaptic Plasticity and Prevents Spatial Memory Deficits: Role of PPARγ and AMPK. Int J Neuropsychopharmacol 25:498-511 (2022). PubMed: 35152284
- Zhang K et al. Electroacupuncture Ameliorates Depression-Like Behaviour in Rats by Enhancing Synaptic Plasticity via the GluN2B/CaMKII/CREB Signalling Pathway. Evid Based Complement Alternat Med 2021:2146001 (2021). PubMed: 34777532