Anti-NMDAR2B 抗体 [EPR19295] (ab183942)
![Western blot - Anti-NMDAR2B antibody [EPR19295] (ab183942)](https://www.abcam.co.jp/ps/products/183/ab183942/Images/ab183942-258210-anti-nmdar2b-antibody-epr19295-western-blot.jpg "Western blot - Anti-NMDAR2B antibody [EPR19295] (ab183942)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19295] to NMDAR2B
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-NMDAR2B antibody [EPR19295]
NMDAR2B 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR19295] to NMDAR2B -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Human hippocampus lysate; Mouse brain and hippocampus lysates; Rat brain and hippocampus lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR19295 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab183942の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 166 kDa (predicted molecular weight: 166 kDa).
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| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 166 kDa (predicted molecular weight: 166 kDa). |
ターゲット情報
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機能
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. -
組織特異性
Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia. -
配列類似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily. -
細胞内局在
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2904 Human
- Entrez Gene: 14812 Mouse
- Entrez Gene: 24410 Rat
- Omim: 138252 Human
- SwissProt: Q13224 Human
- SwissProt: Q01097 Mouse
- SwissProt: Q00960 Rat
- Unigene: 654430 Human
see all -
別名
- AW490526 antibody
- EIEE27 antibody
- Glutamate [NMDA] receptor subunit epsilon 2 antibody
see all
画像
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Western blot - Anti-NMDAR2B antibody [EPR19295] (ab183942)All lanes : Anti-NMDAR2B antibody [EPR19295] (ab183942) at 1/1000 dilution
Lane 1 : Human hippocampus lysate
Lane 2 : Human fetal liver lysate
Lane 3 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 4 : Mouse liver lysate
Lane 5 : Mouse kidney lysate
Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 7 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
Lane 8 : Mouse brain lysate
Lane 9 : Mouse hippocampus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 166 kDa
Observed band size: 166 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1,2,3,4,5,6 and 7: 30 seconds; Lane 8 and 9: 8 seconds.
The expression profile observed is consistent with what has been described in the literature (PMID:9252405; PMID:15470155).
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![Western blot - Anti-NMDAR2B antibody [EPR19295] (ab183942)](https://www.abcam.co.jp/ps/products/183/ab183942/Images/ab183942-258209-anti-nmdar2b-antibody-epr19295-western-blot.jpg)
Western blot - Anti-NMDAR2B antibody [EPR19295] (ab183942)All lanes : Anti-NMDAR2B antibody [EPR19295] (ab183942) at 1/5000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat hippocampus lysate
Lane 3 : Rat liver lysate
Lane 4 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 166 kDa
Observed band size: 166 kDa
Exposure time: 8 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID:9252405; PMID:15470155).
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![Anti-NMDAR2B antibody [EPR19295] (ab183942)](https://www.abcam.co.jp/ps/products/183/ab183942/Images/ab183942-3-benefits-of-recombinant-antibodies.png)
Anti-NMDAR2B antibody [EPR19295] (ab183942)
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (12)
ab183942 は 12 報の論文で使用されています。
- Yokokawa T et al. Altered expression of synaptic proteins and adhesion molecules in the hippocampus and cortex following the onset of diabetes in nonobese diabetic mice. Physiol Rep 11:e15673 (2023). PubMed: 37078449
- Whittsette AL et al. The endoplasmic reticulum membrane complex promotes proteostasis of GABAA receptors. iScience 25:104754 (2022). PubMed: 35938049
- Giesen J et al. Hippocampal AMPA- and NMDA-induced cGMP signals are mainly generated by NO-GC2 and are under tight control by PDEs 1 and 2. Eur J Neurosci 55:18-31 (2022). PubMed: 34902209
- Xu L et al. Deficits in N-Methyl-D-Aspartate Receptor Function and Synaptic Plasticity in Hippocampal CA1 in APP/PS1 Mouse Model of Alzheimer's Disease. Front Aging Neurosci 13:772980 (2021). PubMed: 34916926
- Xu J et al. Low-Dose Copper Exposure Exacerbates Depression-Like Behavior in ApoE4 Transgenic Mice. Oxid Med Cell Longev 2021:6634181 (2021). PubMed: 33833851