Anti-NMDAR1 抗体 - Neuronal Marker (ab17345)
Key features and details
- Rabbit polyclonal to NMDAR1
- Suitable for: IHC (PFA fixed), WB
- Reacts with: Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-NMDAR1 antibody - Neuronal Marker
NMDAR1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NMDAR1 -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab274377.
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アプリケーション
適用あり: IHC (PFA fixed), WBmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide corresponding to Rat NMDAR1 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab17568) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab17345の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC (PFA fixed) |
Use at an assay dependent concentration.
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| WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 105 kDa).
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| 特記事項 |
|---|
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IHC (PFA fixed)
Use at an assay dependent concentration. |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 105 kDa). |
ターゲット情報
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機能
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors. -
配列類似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily. -
翻訳後修飾
NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity. -
細胞内局在
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities. - Information by UniProt
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参照データベース
- Entrez Gene: 2902 Human
- Entrez Gene: 14810 Mouse
- Entrez Gene: 24408 Rat
- Omim: 138249 Human
- SwissProt: Q05586 Human
- SwissProt: P35438 Mouse
- SwissProt: P35439 Rat
- Unigene: 558334 Human
see all -
別名
- GluN1 antibody
- Glutamate [NMDA] receptor subunit zeta-1 antibody
- Glutamate receptor ionotropic N methyl D aspartate 1 antibody
see all
画像
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Western blot - Anti-NMDAR1 antibody - Neuronal Marker (ab17345)All lanes : Anti-NMDAR1 antibody - Neuronal Marker (ab17345) at 2 µg/ml
Lane 1 : HEK293 Whole Cell Lysate Transfected with SHAM
Lane 2 : HEK293 Whole Cell Lysate Transfected with NR1-1a/NR2A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 105 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Additional bands at: 54 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
NMDAR1 has a predicted molecular weight of 105kDa; however it has a number of potential glycosylation and phosphorylation sites which may affect the migration of the protein (SwissProt data). In addition, a band at 37kDa has been observed (data not shown). -
Immunohistochemistry (PFA fixed) - Anti-NMDAR1 antibody - Neuronal Marker (ab17345)This image is courtesy of Sophie Pezet, Univ London Kings Coll, United Kingdomab17345 Rabbit polyclonal to NMDAR1 immunofluorescent staining in the rat brain cortex. Primary antibody ab17345 was incubated at room temperature overnight at 0.5µg/ml. Preincubation with the immunizing peptide (ab17568) resulted in complete blocking of the primary antibody staining. Anti rabbit alexa fluor 488 secondary antibody was used at 1/1000 (incubated for 2h at room temperature). Protocol details: rat tissue was intracardially perfusion fixed with 4% PFA followed by an overnight post fixation in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. As expected NMDAR1 staining is observed in the soma, axon and dendrites of neurons.
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Western blot - Anti-NMDAR1 antibody - Neuronal Marker (ab17345)Anti-NMDAR1 antibody - Neuronal Marker (ab17345) at 2 µg/ml + HEK293 cell lysate co-transfected
with NR1 and NR2A recombinant protein at 50 µg
Predicted band size: 105 kDa
Observed band size: ~110 kDa why is the actual band size different from the predicted?HEK293 cells were co-transfected with NMDAR1 (expected MW ~ 105kDa) and NMDAR2A (expected MW ~ 180kDa). ab17345 NMDAR1 antibody specifically recognises NMDAR1 protein and does not recognise NMDAR2A protein. ab17345 was incubated overnight at 4°C at 2µg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (44)
ab17345 は 44 報の論文で使用されています。
- Pang S et al. Integrin β1/FAK/SRC signal pathway is involved in autism spectrum disorder in Tspan7 knockout rats. Life Sci Alliance 6:N/A (2023). PubMed: 36625203
- Weng ZJ et al. Spinal cord astrocyte P2X7Rs mediate the inhibitory effect of electroacupuncture on visceral hypersensitivity of rat with irritable bowel syndrome. Purinergic Signal 19:43-53 (2023). PubMed: 35389158
- Serwach K et al. STIM2 regulates NMDA receptor endocytosis that is induced by short-term NMDA receptor overactivation in cortical neurons. Cell Mol Life Sci 80:368 (2023). PubMed: 37989792
- Visini G et al. The effects of preventative cannabidiol in a male neuregulin 1 mouse model of schizophrenia. Front Cell Neurosci 16:1010478 (2022). PubMed: 36406747
- Dasdelen MF et al. A Novel Theanine Complex, Mg-L-Theanine Improves Sleep Quality via Regulating Brain Electrochemical Activity. Front Nutr 9:874254 (2022). PubMed: 35449538