Anti-NM23A 抗体 (ab154547)
Key features and details
- Rabbit polyclonal to NM23A
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-NM23A antibody
NM23A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NM23A -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Cow, Pig, Monkey
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免疫原
Recombinant fragment, corresponding to a region within amino acids 1-152 of Human NM23A (Uniprot ID: P15531).
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ポジティブ･コントロール
- 293T, H1299, HeLa, HepG2, MOLT4, Raji, Mouse brain whole cell lysate; HeLa cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154547の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 17 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 17 kDa. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Major role in the synthesis of nucleoside triphosphates other than ATP. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. -
組織特異性
Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation. Isoform 3 is ubiquitously expressed. -
配列類似性
Belongs to the NDK family. -
細胞内局在
Cytoplasm. Nucleus. Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA. - Information by UniProt
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参照データベース
- Entrez Gene: 4830 Human
- Entrez Gene: 18102 Mouse
- Entrez Gene: 191575 Rat
- Omim: 156490 Human
- SwissProt: P15531 Human
- SwissProt: P15532 Mouse
- SwissProt: Q05982 Rat
- Unigene: 463456 Human
see all -
別名
- AWD antibody
- AWD, drosophila, homolog of antibody
- GAAD antibody
see all
画像
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Western blot - Anti-NM23A antibody (ab154547)All lanes : Anti-NM23A antibody (ab154547) at 1/1000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : MOLT4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 17 kDa
12% SDS PAGE -
Western blot - Anti-NM23A antibody (ab154547)Anti-NM23A antibody (ab154547) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 17 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-NM23A antibody (ab154547)Immunofluorescent analysis of methanol-fixed HeLa cells labeling NM23A with ab154547 at 1/100 dilution. Lower panel merged with DNA probe.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab154547 は 4 報の論文で使用されています。
- Wertz MH et al. Genome-wide In Vivo CNS Screening Identifies Genes that Modify CNS Neuronal Survival and mHTT Toxicity. Neuron 106:76-89.e8 (2020). PubMed: 32004439
- Yang M et al. Electro-Acupuncture Promotes Accumulation of Paclitaxel by Altering Tumor Microvasculature and Microenvironment in Breast Cancer of Mice. Front Oncol 9:576 (2019). PubMed: 31312613
- Tang M et al. Inhibition effects of Yuxiao San combined with cisplatin on transplanted tumor growths via upregulation of nm-23 and downregulation of K-ras in Lewis lung cancer mice. Oncol Lett 17:1267-1273 (2019). PubMed: 30655894
- Zhu B et al. Downregulation of Krüppel-like factor 1 inhibits the metastasis and invasion of cervical cancer cells. Mol Med Rep 18:3932-3940 (2018). PubMed: 30132534