Anti-NLRX1 抗体 (ab107611)
Key features and details
- Rabbit polyclonal to NLRX1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Horse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-NLRX1 antibody
NLRX1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NLRX1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Horse, Human
交差が予測される動物種: Cow, Dog
-
免疫原
Synthetic peptide, corresponding to a region within amino acids 580-630 of Human NLRX1 (NP_078894.2).
-
ポジティブ･コントロール
- Mouse heart tissue lysate; Human brain tissue
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term. -
バッファー
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab107611の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 5 - 7 µg/ml. Predicted molecular weight: 108 kDa.
|
|
| IHC-P |
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 5 - 7 µg/ml. Predicted molecular weight: 108 kDa. |
|
IHC-P
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Participates in antiviral signaling. Acts as a negative regulator of MAVS-mediated antiviral responses, through the inhibition of the virus-induced RLH (RIG-like helicase)-MAVS interaction (PubMed:18200010). Has no inhibitory function on NF-Kappa-B and type 1 interferon signaling pathways, but enhances NF-Kappa-B and JUN N-terminal kinase dependent signaling through the production of reactive oxygen species (PubMed:18219313). -
組織特異性
Ubiquitously expressed. Strongest expression in mammary gland, heart and muscle. Detected in HELA, 293T, THP-1, HL60, RAJI, and JURKAT cell lines (at protein level). -
配列類似性
Belongs to the NLRP family.
Contains 7 LRR (leucine-rich) repeats.
Contains 1 NACHT domain. -
細胞内局在
Mitochondrion outer membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 539974 Cow
- Entrez Gene: 100071383 Horse
- Entrez Gene: 79671 Human
- Entrez Gene: 270151 Mouse
- Omim: 611947 Human
- SwissProt: Q86UT6 Human
- SwissProt: Q3TL44 Mouse
- Unigene: 524082 Human
see all -
別名
- Caterpiller protein 11.3 antibody
- CLR11.3 antibody
- DLNB26 antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRX1 antibody (ab107611)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human heart tissue labelling NLRX1 with ab107611 at 10µg/ml. Staining was enhanced by boiling tissue sections in 10mM sodium citrate buffer, pH6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
-
Western blot - Anti-NLRX1 antibody (ab107611)All lanes : Anti-NLRX1 antibody (ab107611) at 5 µg/ml
Lane 1 : Mouse heart tissue lysate in absence immunizing peptide
Lane 2 : Mouse heart tissue lysate in presence of immunuzing peptide
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti-Rabbit Ig HRP
Developed using the ECL technique.
Predicted band size: 108 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRX1 antibody (ab107611)ab107611, at 5µg, staining NLRX1 in formalin-fixed, paraffin-embedded Human brain tissue by immunohistochemisty.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (3)
ab107611 は 3 報の論文で使用されています。
- Wang Q et al. NLRX1 increases human retinal pigment epithelial autophagy and reduces H2O2-induced oxidative stress and inflammation by suppressing FUNDC1 phosphorylation and NLRP3 activation. Allergol Immunopathol (Madr) 51:177-186 (2023). PubMed: 36617838
- Zhang H et al. NLRX1 Deletion Increases Ischemia-Reperfusion Damage and Activates Glucose Metabolism in Mouse Heart. Front Immunol 11:591815 (2020). PubMed: 33362773
- Stokman G et al. NLRX1 dampens oxidative stress and apoptosis in tissue injury via control of mitochondrial activity. J Exp Med 214:2405-2420 (2017). PubMed: 28626071