Anti-Nkx2.2 抗体 [NX2/294] (ab187375)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] (ab187375)](https://www.abcam.co.jp/ps/products/187/ab187375/Images/ab187375-395234-anti-nkx2-2-antibody-nx2-294-immunohistochemistry-evings-sarcoma-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] (ab187375)")
Key features and details
- Mouse monoclonal [NX2/294] to Nkx2.2
- Suitable for: IHC-P
- Reacts with: Rat, Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-Nkx2.2 antibody [NX2/294]
Nkx2.2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [NX2/294] to Nkx2.2 -
由来種
Mouse -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Rat, Human -
免疫原
Recombinant full length protein corresponding to Human Nkx2.2 aa 1-273.
Database link: O95096 -
ポジティブ･コントロール
- IHC-P: Human and rat pancreas and Human Ewing sarcoma tissues.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
精製度
Protein A/G purified -
ポリ/モノ
モノクローナル -
クローン名
NX2/294 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187375の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Performed for 30 minutes at RT Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| 特記事項 |
|---|
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IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Performed for 30 minutes at RT Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
ターゲット情報
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機能
May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. -
配列類似性
Belongs to the NK-2 homeobox family.
Contains 1 homeobox DNA-binding domain. -
ドメイン
The homeodomain is essential for interaction with OLIG2. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 4821 Human
- Entrez Gene: 366214 Rat
- Omim: 604612 Human
- SwissProt: O95096 Human
- Unigene: 516922 Human
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別名
- Homeobox protein NK 2 homolog B antibody
- Homeobox protein NK-2 homolog B antibody
- Homeobox protein Nkx 2.2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] (ab187375)
Immunohistochemical analysis of paraffin embedded human Ewing's Sarcoma tissue labeling Nkx2.2 with ab187375 at 2 µg/mL.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] (ab187375)](https://www.abcam.co.jp/ps/products/187/ab187375/Images/ab187375-395233-anti-nkx2-2-antibody-nx2-294-immunohistochemistry-pancreas-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] (ab187375)Immunohistochemical analysis of paraffin embedded rat pancreas tissue labeling Nkx2.2 with ab187375 at 2 µg/mL.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] (ab187375)](https://www.abcam.co.jp/ps/products/187/ab187375/Images/ab187375-219568-anti-nkx22-antibody-nx2-294-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [NX2/294] (ab187375)Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling Nkx2.2 with ab187375 at 2 µg/mL.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab187375 は 4 報の論文で使用されています。
- Kitagawa T et al. AT-hook DNA-binding motif-containing protein one knockdown downregulates EWS-FLI1 transcriptional activity in Ewing's sarcoma cells. PLoS One 17:e0269077 (2022). PubMed: 36194562
- Ross NT et al. CPSF3-dependent pre-mRNA processing as a druggable node in AML and Ewing's sarcoma. Nat Chem Biol 16:50-59 (2020). PubMed: 31819276
- Nabet B et al. Rapid and direct control of target protein levels with VHL-recruiting dTAG molecules. Nat Commun 11:4687 (2020). PubMed: 32948771
- Huang W et al. Origins and Proliferative States of Human Oligodendrocyte Precursor Cells. Cell 182:594-608.e11 (2020). PubMed: 32679030