Anti-NKp30 抗体 [EPR14509] (ab186425)
![Western blot - Anti-NKp30 antibody [EPR14509] (ab186425)](https://www.abcam.co.jp/ps/products/186/ab186425/Images/ab186425-220427-anti-nk-p30-antibody-epr14509-western-blot.jpg "Western blot - Anti-NKp30 antibody [EPR14509] (ab186425)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14509] to NKp30
- Suitable for: IP, WB
- Reacts with: Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-NKp30 antibody [EPR14509]
NKp30 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR14509] to NKp30 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- THP1, NCI-H460, K562 and rat brain lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR14509 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186425の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
1/50 - 1/70.
|
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| WB |
1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
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| 特記事項 |
|---|
|
IP
1/50 - 1/70. |
|
WB
1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
ターゲット情報
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機能
Cytotoxicity-activating receptor that contributes to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. Engagement of NCR3 by BAG6 also promotes dendritic cell (DC) maturation, both through killing those DCs that did not properly acquire a mature phenotype, and inducing NK cells to release TNFA and IFNG, which promotes DC maturation. -
組織特異性
Selectively expressed by all resting and activated NK cells and weakly expressed in spleen. -
配列類似性
Belongs to the natural cytotoxicity receptor (NCR) family.
Contains 1 Ig-like (immunoglobulin-like) domain. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 259197 Human
- Entrez Gene: 294251 Rat
- Omim: 611550 Human
- SwissProt: O14931 Human
- SwissProt: Q8CFD9 Rat
- Unigene: 509513 Human
- Unigene: 203206 Rat
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別名
- 1C7 antibody
- Activating natural killer receptor p30 antibody
- Activating NK A1 receptor antibody
see all
画像
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Western blot - Anti-NKp30 antibody [EPR14509] (ab186425)All lanes : Anti-NKp30 antibody [EPR14509] (ab186425) at 1/1000 dilution
Lane 1 : THP1 cell lysate
Lane 2 : NCI-H460 lysate
Lane 3 : Rat brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa -
![Western blot - Anti-NKp30 antibody [EPR14509] (ab186425)](https://www.abcam.co.jp/ps/products/186/ab186425/Images/ab186425-220428-anti-nk-p30-antibody-epr14509-western-blot.jpg)
Western blot - Anti-NKp30 antibody [EPR14509] (ab186425)Anti-NKp30 antibody [EPR14509] (ab186425) at 1/1000 dilution + K562 lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa -
![Immunoprecipitation - Anti-NKp30 antibody [EPR14509] (ab186425)](https://www.abcam.co.jp/ps/products/186/ab186425/Images/ab186425-220429-anti-nk-p30-antibody-epr14509-immunoprecipitation.jpg)
Immunoprecipitation - Anti-NKp30 antibody [EPR14509] (ab186425)Western blot analysis of NK-p30 in K562 cell lysate immunoprecipitated using ab186425 at 1/50 dilution (Lane 1). Lane 2: PBS instead of K562 lysate. Anti-Rabbit IgG (HRP) specific to the non-reduced form of IgG was used as a secondary antibody at 1/1500 dilution.
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![Anti-NKp30 antibody [EPR14509] (ab186425)](https://www.abcam.co.jp/ps/products/186/ab186425/Images/ab186425-4-benefits-of-recombinant-antibodies.png)
Anti-NKp30 antibody [EPR14509] (ab186425)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (3)
ab186425 は 3 報の論文で使用されています。
- Wang J et al. WTAP enhances the instability of SYTL1 mRNA caused by YTHDF2 in bladder cancer. Histol Histopathol 39:633-646 (2024). PubMed: 37933909
- Adithan A et al. Biological effects of cyclosporin A on CD3-CD161+ and CD3+CD161+ lymphocytes. Mol Cell Biochem N/A:N/A (2019). PubMed: 31020492
- Qiu Y et al. Lactobacillus plantarum Enhanced IL-22 Production in Natural Killer (NK) Cells That Protect the Integrity of Intestinal Epithelial Cell Barrier Damaged by Enterotoxigenic Escherichia coli. Int J Mol Sci 18:N/A (2017). PubMed: 29137183