Anti-NKG2D 抗体 [EPR24072-342] - BSA and Azide free (ab302908)

This data was developed using AB302907, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling NKG2D with ab302907 at 1/2000 dilution (0.258 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human tonsil is observed. The section was incubated with ab302907 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: A ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval solution 2) for 20 mins.

This data was developed using AB302907, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human NK/T cell lymphoma tissue labeling NKG2D with ab302907 at 1/2000 dilution (0.258 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human NK/T cell lymphoma was observed. The section was incubated with ab302907 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: A ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval solution 2) for 20 mins.

This data was developed using AB302907, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling NKG2D with ab302907 at 1/2000 dilution (0.258 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). The section was incubated with ab302907 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Negative control: no staining on human cardiac muscle is observed.

Secondary antibody only control: A ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval solution 2) for 20 mins.

This data was developed using AB302907, the same antibody clone in a different buffer formulation. 

Multichannel (Multiplex) immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections of human tonsil tissue labeling NKG2D, NKG2a, and CD8 alpha.

Panel A: merged staining of anti-CD8 alpha (red; Opal™690), anti-NKG2A (gray; Opal™520) and anti-NKG2D (green; Opal™570) on human tonsil.

Panel B: anti-NKG2D (ab302907 at 1:2000 dilution (0.258 μg/ml)) signal on NK cells and T cell subsets.

Panel C: anti-NKG2A (ab260035 at 1:2000 dilution (0.283 μg/ml)) signal on NK cells.

Panel D: anti-CD8 alpha (ab178089 at 1:100 dilution (0.83 μg/ml)) signal on T cell subsets.

Opal Polymer HRP Ms + Rb was used as secondary antibody

The section was incubated in three rounds of staining: in the order of ab178089, ab260035, and ab302907 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Nuclear counterstain was DAPI.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.