Anti-NKG2D 抗体 (ab36136)
Key features and details
- Goat polyclonal to NKG2D
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NKG2D antibody
NKG2D 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to NKG2D -
由来種
Goat -
アプリケーション
適用あり: WB, Flow Cytmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide:
KVYSKEDQDLLK
, corresponding to Internal sequence amino acids 136-147 of Human NKG2D -
ポジティブ･コントロール
- WB: MOLT4 whole cell lysate. Flow Cyt: Human peripheral blood monocytes
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab36136の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 25.3 kDa).
1 hour primary incubation is recommended for this product. |
|
| Flow Cyt |
Use a concentration of 10 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 25.3 kDa). 1 hour primary incubation is recommended for this product. |
|
Flow Cyt
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Receptor for MICA, MICB, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. Involved in the immune surveillance exerted by T- and B-lymphocytes. -
組織特異性
Natural killer cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Also detected in gamma-delta cells and CD8+ alpha-beta T-cells. Expressed in interferon-producing killer dendritic cells (IKDCs). -
配列類似性
Contains 1 C-type lectin domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 100528032 Human
- Entrez Gene: 22914 Human
- Entrez Gene: 27007 Mouse
- Entrez Gene: 24934 Rat
- Omim: 611817 Human
- SwissProt: P26718 Human
- SwissProt: O54709 Mouse
- SwissProt: O70215 Rat
see all -
別名
- CD314 antibody
- CD314 antigen antibody
- D12S2489E antibody
see all
画像
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Flow Cytometry - Anti-NKG2D antibody (ab36136)Flow cytometric analysis of human peripheral blood monocytes. Primary incubation 30 minutes (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL).
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Western blot - Anti-NKG2D antibody (ab36136)All lanes : Anti-NKG2D antibody (ab36136) at 0.5 µg/ml
Lane 1 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate
Lane 2 : MOLT-4 whole cell lysate plus peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 25.3 kDaLysates in RIPA buffer.
Detected by chemiluminescence.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab36136 は 4 報の論文で使用されています。
- Wang R et al. Autophagy-mediated NKG2D internalization impairs NK cell function and exacerbates radiation pneumonitis. Front Immunol 14:1250920 (2023). PubMed: 38077388
- Kamei R et al. Expression levels of UL16 binding protein 1 and natural killer group 2 member D affect overall survival in patients with gastric cancer following gastrectomy. Oncol Lett 15:747-754 (2018). PubMed: 29391893
- Li Y et al. Human iPSC-Derived Natural Killer Cells Engineered with Chimeric Antigen Receptors Enhance Anti-tumor Activity. Cell Stem Cell 23:181-192.e5 (2018). PubMed: 30082067
- Park YP et al. Complex regulation of human NKG2D-DAP10 cell surface expression: opposing roles of the {gamma}c cytokines and TGF-{beta}1. Blood 118:3019-27 (2011). WB ; Human . PubMed: 21816829