Anti-NIPSNAP1 抗体 (ab67302)
Key features and details
- Rabbit polyclonal to NIPSNAP1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-NIPSNAP1 antibody
NIPSNAP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NIPSNAP1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human Brain Tissue Lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab67302の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
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| IHC-P |
Use a concentration of 2.5 µg/ml.
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| WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 20 µg/ml. |
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IHC-P
Use a concentration of 2.5 µg/ml. |
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). |
ターゲット情報
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関連性
NIPSNAP is a member of an evolutionarily well conserved gene family and has a strong sequence similarity to the central portion of a protein encoded by C. elegans chromosome III between a 4-nitrophenylphosphatase (NIP) domain and non-neuronal SNAP25-like protein. Recent studies have indicated that NIPSNAP is involved in the regulation of the Ca2+-selective transient receptor potential vanilloid channel 6 (TRPV6). NIPSNAP1 associates with TRPV6 at the plasma membrane and inhibits TRPV6 currents. Other studies show that NIPSNAP’s expression is reduced in the phenylketonuria (PKU) mouse brain, suggesting that NIPSNAP may play a role in memory. -
細胞内局在
Mitochondrial -
参照データベース
- Entrez Gene: 8508 Human
- Entrez Gene: 18082 Mouse
- Entrez Gene: 360971 Rat
- Omim: 603249 Human
- SwissProt: Q9BPW8 Human
- SwissProt: O55125 Mouse
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別名
- NIPSNAP 1 antibody
- Nipsnap homolog 1 (C. elegans) antibody
- Protein NipSnap homolog 1 antibody
画像
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Western blot - Anti-NIPSNAP1 antibody (ab67302)Lane 1 : Anti-NIPSNAP1 antibody (ab67302) at 0.5 µg/ml
Lane 2 : Anti-NIPSNAP1 antibody (ab67302) at 1 µg/ml
All lanes : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 33 kDa
Observed band size: 33 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIPSNAP1 antibody (ab67302)ab67302 at 2.5 µg/ml staining NIPSNAP1 in human prostate tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).
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Immunocytochemistry/ Immunofluorescence - Anti-NIPSNAP1 antibody (ab67302)Immunofluorescence of NIPSNAP in Human Brain cells using ab67302 at 20 ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab67302 は 3 報の論文で使用されています。
- Munson MJ et al. GAK and PRKCD are positive regulators of PRKN-independent mitophagy. Nat Commun 12:6101 (2021). PubMed: 34671015
- Princely Abudu Y et al. NIPSNAP1 and NIPSNAP2 Act as "Eat Me" Signals for Mitophagy. Dev Cell 49:509-525.e12 (2019). PubMed: 30982665
- Uthaiah RC & Hudspeth AJ Molecular anatomy of the hair cell's ribbon synapse. J Neurosci 30:12387-99 (2010). PubMed: 20844134