Anti-NIPBL 抗体 [KT55]

• Flow Cyt

Unknown

Flow Cytometry - Anti-NIPBL antibody [KT55] (AB106768)

Overlay histogram showing HepG2 cells stained with ab106768 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab106768, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rat IgG (H+L) (ab150165) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIPBL antibody [KT55] (AB106768)

IHC-P was performed using formalin-fixed paraffin-embedded human stomach tissue sections. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with NIPBL antibody (KT55) at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG (which cross reacts with rat IgG) was used as the secondary antibody. Diaminobenzidine (DAB) was used as the chromogen. Glandular cells are positively stained at the nuclei. The section was counterstained with hematoxylin.

A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.

• WB

PubMed

Western blot - Anti-NIPBL antibody [KT55] (AB106768)

Gene silencing efficiencies in HCT116 cells. Semi-quantitative western blot presenting residual proteins levels following NIPBL silencing with pooled siRNA duplexes in HCT116 cells. Cyclophilin-B as loading control. Residual protein levels are presented normalized to the respective loading control and are presented relative to siControl, which is set at 1.00.

false

T.R Leylek et al Sci Rep . 2020 Jan 17;10(1):592. doi: 10.1038/s41598-020-57530-9.

• WB

PubMed

Western blot - Anti-NIPBL antibody [KT55] (AB106768)

Western blot analysis of U2OS whole-cell extracts prepared from cells treated with siRNA targeting NIPBL. NTC, non-targeting control. α-Tubulin was used as a loading control.

true

C Meisenberg et al Molecular Cell 2019 Jan 17;73(2):212-223.e7. doi: 10.1016/j.molcel.2018.11.001. Epub 2018 Dec 13.