Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 抗体 (ab41174)
Key features and details
- Rabbit polyclonal to Nicotinic Acetylcholine Receptor beta 2/CHRNB2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody
Nicotinic Acetylcholine Receptor beta 2/CHRNB2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Nicotinic Acetylcholine Receptor beta 2/CHRNB2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Dilute the lyophilized antibody with 0.1 ml of 10 mg/ml BSA in PBS for the equivalent of whole antiserum -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituents: PBS, 1% BSA, Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab41174の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/400. Detects a band of approximately 52 kDa (predicted molecular weight: 57 kDa). With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
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ICC/IF |
1/200.
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特記事項 |
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WB
1/400. Detects a band of approximately 52 kDa (predicted molecular weight: 57 kDa). With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk. |
ICC/IF
1/200. |
ターゲット情報
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機能
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. -
関連疾患
Defects in CHRNB2 are the cause of nocturnal frontal lobe epilepsy type 3 (ENFL3) [MIM:605375]. ENFL3 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements. -
配列類似性
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta-2/CHRNB2 sub-subfamily. -
細胞内局在
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1141 Human
- Omim: 118507 Human
- SwissProt: P17787 Human
- Unigene: 2306 Human
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別名
- Acetylcholine receptor beta 2 neural antibody
- ACHB2_HUMAN antibody
- ACHN antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody (ab41174)ICC/IF image of ab41174 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41174, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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All lanes : Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody (ab41174) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Additional bands at: 46 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutesThe band observed at 52 kDa could potentially be a cleaved form of Nicotinic Acetylcholine Receptor beta 2/CHRNB2 due to the presence of a 25 amino acid signal peptide.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab41174 は 2 報の論文で使用されています。
- Qin C et al. CHRNB2 represses pancreatic cancer migration and invasion via inhibiting β-catenin pathway. Cancer Cell Int 22:340 (2022). PubMed: 36344976
- Seoane-Collazo P et al. Central nicotine induces browning through hypothalamic ? opioid receptor. Nat Commun 10:4037 (2019). PubMed: 31492869