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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Nicastrin 抗体 [EPR2575Y] (ab68145)

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Western blot - Anti-Nicastrin antibody [EPR2575Y] (ab68145)
  • Western blot - Anti-Nicastrin antibody [EPR2575Y] (ab68145)
  • Western blot - Anti-Nicastrin antibody [EPR2575Y] (ab68145)
  • Anti-Nicastrin antibody [EPR2575Y] (ab68145)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2575Y] to Nicastrin
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Nicastrin antibody [EPR2575Y]
    Nicastrin 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR2575Y] to Nicastrin

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: ICC/IF or IHC-P

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human Nicastrin aa 650-750 (C terminal). The exact sequence is proprietary.

  • ポジティブ･コントロール

    • WB: HEK293T, HeLa, Jurkat and HL-60 cell lysates; C6 cell lysate; Neuro-2a cell lysate; U-87 MG and SH-SY5Y cell lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab68145の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/2000. Detects a band of approximately 100-120, 130-160 kDa (predicted molecular weight: 78 kDa).
特記事項
WB
1/1000 - 1/2000. Detects a band of approximately 100-120, 130-160 kDa (predicted molecular weight: 78 kDa).
追加情報
Is unsuitable for ICC/IF or IHC-P.

ターゲット情報

  • 機能

    Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex.

  • 組織特異性

    Widely expressed.

  • 配列類似性

    Belongs to the nicastrin family.

  • 細胞内局在

    Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Anterior pharynx defective 2 antibody
    • APH 2 antibody
    • APH2 antibody
    • ATAG1874 antibody
    • KIAA0253 antibody
    • Ncstn antibody
    • NCT antibody
    • NICA_HUMAN antibody
    • Nicastrin antibody
    • RP11 517F10.1 antibody
    • RP11517F101 antibody
    see all

画像

  • Western blot - Anti-Nicastrin antibody [EPR2575Y] (ab68145)
    Western blot - Anti-Nicastrin antibody [EPR2575Y] (ab68145)
    All lanes : Anti-Nicastrin antibody [EPR2575Y] (ab68145) at 1/1000 dilution

    Lane 1 : C6 (rat glial tumor glial cell) whole cell lysate
    Lane 2 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
    Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate
    Lane 4 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 78 kDa
    Observed band size: 100-140 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: Lane 1, 4: 180 seconds; Lane 2, 3: 15 seconds.

    The bands observed is consistency with literature (PMID: 15322084).
    The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

  • Western blot - Anti-Nicastrin antibody [EPR2575Y] (ab68145)
    Western blot - Anti-Nicastrin antibody [EPR2575Y] (ab68145)
    All lanes : Anti-Nicastrin antibody [EPR2575Y] (ab68145) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : NCSTN knockout HEK293T cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : HL-60 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 78 kDa
    Observed band size: 100-125 kDa why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab68145 observed at 100-125 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab68145 Anti-Nicastrin antibody [EPR2575Y] was shown to specifically react with Nicastrin in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266602 (knockout cell lysate ab258061) was used. Wild-type and Nicastrin knockout samples were subjected to SDS-PAGE. ab68145 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Nicastrin antibody [EPR2575Y] (ab68145)
    Western blot - Anti-Nicastrin antibody [EPR2575Y] (ab68145)
    All lanes : Anti-Nicastrin antibody [EPR2575Y] (ab68145) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HL-60 cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP conjugated Goat anti-Rabbit at 1/2000 dilution

    Predicted band size: 78 kDa
    Additional bands at: 100-120 kDa (possible isoform), 130-160 kDa (possible isoform)

  • Anti-Nicastrin antibody [EPR2575Y] (ab68145)
    Anti-Nicastrin antibody [EPR2575Y] (ab68145)

参考文献 (1)

ab68145 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab68145 は 1 報の論文で使用されています。

  • Hsu YC  et al. De-Glycyrrhizinated Licorice Extract Attenuates High Glucose-Stimulated Renal Tubular Epithelial-Mesenchymal Transition via Suppressing the Notch2 Signaling Pathway. Cells 9:N/A (2020). PubMed: 31948095

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