Anti-Nicastrin 抗体 (ab45425)
Key features and details
- Rabbit polyclonal to Nicastrin
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Nicastrin antibody
Nicastrin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Nicastrin -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Nicastrin.
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ポジティブ･コントロール
- IHC-P: Human brain tissue. Human breast carcinoma tissue. ICC/IF: Human brain cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab45425の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
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| IHC-P | (1) |
Use a concentration of 2.5 µg/ml.
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| WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 78 kDa.
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 20 µg/ml. |
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IHC-P
Use a concentration of 2.5 µg/ml. |
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WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 78 kDa. |
ターゲット情報
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機能
Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the nicastrin family. -
細胞内局在
Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 23385 Human
- Omim: 605254 Human
- SwissProt: Q92542 Human
- Unigene: 517249 Human
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別名
- Anterior pharynx defective 2 antibody
- APH 2 antibody
- APH2 antibody
see all
画像
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Western blot - Anti-Nicastrin antibody (ab45425)Lane 1 : Anti-Nicastrin antibody (ab45425) at 0.5 µg/ml
Lane 2 : Anti-Nicastrin antibody (ab45425) at 1 µg/ml
All lanes : Human brain tissue lysate
Predicted band size: 78 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicastrin antibody (ab45425)ab45425 at 2.5 µg/ml staining Nicastrin in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
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Immunocytochemistry/ Immunofluorescence - Anti-Nicastrin antibody (ab45425)Immunofluorescence of Nicastrin in Human Brain cells using ab45425 at 20 ug/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicastrin antibody (ab45425)This image is courtesy of an Abreview submitted by Carl Hobbsab45425 staining Nicastrin in Human breast carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2500 in TBS/BSA/azide) for 16 hours at 21°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab45425 は 5 報の論文で使用されています。
- Shi TW et al. Effects of NCSTN Mutation on Hair Follicle Components in Mice. Dermatology 239:60-71 (2023). PubMed: 35843211
- Takeichi T et al. A novel NCSTN missense mutation in the signal peptide domain causes hidradenitis suppurativa, which has features characteristic of an autoinflammatory keratinization disease. Br J Dermatol 182:491-493 (2020). PubMed: 31421058
- Bannai T et al. Chronic cerebral hypoperfusion shifts the equilibrium of amyloid ß oligomers to aggregation-prone species with higher molecular weight. Sci Rep 9:2827 (2019). PubMed: 30808940
- Lin Q et al. Inhibition of CD9 expression reduces the metastatic capacity of human hepatocellular carcinoma cell line MHCC97-H. Int J Oncol 53:266-274 (2018). PubMed: 29749468
- D'Souza Y et al. Characterization of Aldh2 (-/-) mice as an age-related model of cognitive impairment and Alzheimer's disease. Mol Brain 8:27 (2015). WB, Dot blot ; Mouse . PubMed: 25910195