Anti-Nicalin 抗体 [EPR8529] (ab170100)
![Western blot - Anti-Nicalin antibody [EPR8529] (ab170100)](https://www.abcam.co.jp/ps/products/170/ab170100/Images/ab170100-170037-anti-nicalin-antibody-epr8529-western-blot.jpg "Western blot - Anti-Nicalin antibody [EPR8529] (ab170100)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8529] to Nicalin
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Nicalin antibody [EPR8529]
Nicalin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR8529] to Nicalin -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human Nicalin.
Database link: Q969V3 -
ポジティブ･コントロール
- A431, HeLa or 293T lysate, Human pancreas or uterus tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR8529 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab170100の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning. -
組織特異性
Highly expressed in pancreas and skeletal muscle and, at lower levels, in heart. -
配列類似性
Belongs to the nicastrin family. -
細胞内局在
Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 56926 Human
- Entrez Gene: 103425 Mouse
- Entrez Gene: 314648 Rat
- Omim: 609156 Human
- SwissProt: Q969V3 Human
- SwissProt: Q8VCM8 Mouse
- SwissProt: Q5XIA1 Rat
- Unigene: 657032 Human
see all -
別名
- NCLN antibody
- NCLN_HUMAN antibody
- NET59 antibody
see all
画像
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Western blot - Anti-Nicalin antibody [EPR8529] (ab170100)All lanes : Anti-Nicalin antibody [EPR8529] (ab170100) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 60 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicalin antibody [EPR8529] (ab170100)](https://www.abcam.co.jp/ps/products/170/ab170100/Images/ab170100-170036-anti-nicalin-antibody-epr8529-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicalin antibody [EPR8529] (ab170100)Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling Nicalin with ab170100 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicalin antibody [EPR8529] (ab170100)](https://www.abcam.co.jp/ps/products/170/ab170100/Images/ab170100-170035-anti-nicalin-antibody-epr8529-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicalin antibody [EPR8529] (ab170100)Immunohistochemical analysis of paraffin embedded Human uterus tissue labeling Nicalin with ab170100 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Nicalin antibody [EPR8529] (ab170100)](https://www.abcam.co.jp/ps/products/170/ab170100/Images/ab170100-4-benefits-of-recombinant-antibodies.png)
Anti-Nicalin antibody [EPR8529] (ab170100)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab170100 は論文での使用が確認できていません。