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リコンビナントRabMAb

Anti-NgR3 抗体 [EPR11334] (ab174323)

Submit a review Submit a question References (1)
Western blot - Anti-NgR3 antibody [EPR11334] (ab174323)
  • Flow Cytometry (Intracellular) - Anti-NgR3 antibody [EPR11334] (ab174323)
  • Anti-NgR3 antibody [EPR11334] (ab174323)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11334] to NgR3
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-NgR3 antibody [EPR11334]
    NgR3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR11334] to NgR3

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WBmore details
    適用なし: IHC-P or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • SH-SY5Y and MCF-7 cell lysates. K562 cells and cell lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR11334

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab174323の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/5000. Predicted molecular weight: 49 kDa.
特記事項
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/5000. Predicted molecular weight: 49 kDa.
追加情報
Is unsuitable for IHC-P or IP.

ターゲット情報

  • 機能

    May play a role in regulating axonal regeneration and plasticity in the adult central nervous system.

  • 組織特異性

    Predominantly expressed in brain. Expressed at lower levels in kidney, lung, mammary gland, placenta, salivary gland, skeletal muscle and spleen.

  • 配列類似性

    Belongs to the Nogo receptor family.
    Contains 8 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.

  • 細胞内局在

    Cell membrane. Localized to the surface of neurons, including axons.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • DKFZp547J144 antibody
    • NgR 3 antibody
    • NgR3 antibody
    • NGRH 2 antibody
    • NGRH2 antibody
    • NGRL2 antibody
    • Nogo 66 receptor homolog 2 antibody
    • Nogo 66 receptor related protein 3 antibody
    • Nogo receptor like 2 antibody
    • Nogo receptor-like 2 antibody
    • Nogo-66 receptor homolog 2 antibody
    • Nogo-66 receptor-related protein 3 antibody
    • R4RL1_HUMAN antibody
    • Reticulon 4 receptor like 1 antibody
    • Reticulon-4 receptor-like 1 antibody
    • RTN4RL1 antibody
    see all

画像

  • Western blot - Anti-NgR3 antibody [EPR11334] (ab174323)
    Western blot - Anti-NgR3 antibody [EPR11334] (ab174323)
    All lanes : Anti-NgR3 antibody [EPR11334] (ab174323) at 1/1000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : MCF-7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 49 kDa

  • Flow Cytometry (Intracellular) - Anti-NgR3 antibody [EPR11334] (ab174323)
    Flow Cytometry (Intracellular) - Anti-NgR3 antibody [EPR11334] (ab174323)

    Intracellular flow cytometric analysis of permeabilized K562 cells labeling NgR3 with ab174323 at 1/100 dilution (red) compared with a rabbit IgG negative control (green). 

     

     

  • Anti-NgR3 antibody [EPR11334] (ab174323)
    Anti-NgR3 antibody [EPR11334] (ab174323)

参考文献 (1)

ab174323 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab174323 は 1 報の論文で使用されています。

  • Hu H  et al. Effect of ganglioside combined with Chip Jiaji electro-acupuncture on Nogo-NgR signal pathway in SCI rats. Saudi J Biol Sci 28:4132-4136 (2021). PubMed: 34354392

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