Anti-NGF 抗体 - BSA and Azide free (ab6199)

Several different lysates could be used as a positive control. We have used ab6199 with MEF1 cell line in Western blotting. Alternatively it has also been used with rat brain tissue and should also work with mouse or human brain tissue. We have a range of different brain lysates. These can be viewed under the following catalogue numbers:

Brain (Human) Tissue Lysate - adult normal tissue (ab29466)

Brain (Mouse) Whole Cell Lysate - normal tissue (ab30151)

Brain (Rat) Whole Cell Lysate - normal tissue (ab29475)

Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188)

Thank you for contacting Abcam.

I beleive the rabbit IgG control below would work very well for your application.

https://www.abcam.com/Rabbit-IgG-ChIP-Grade-ab37415.html



Also, please see the attachment to find out details of our current promotion, of buy one antibody, get a RabMab for free.

Vielen Dank für Ihre Anfrage.
Bitte geben Sie bei einem Volumen von 500ul Antikörper 500ul Glycerin (Glycerol) dazu.
Das ergibt eine Endkonzentration von 50% Glycerin in 1000ul.
Ich hoffe, der Wetteinsatz ist interessant :-)

Suite à notre conversation téléphonique, je vous envoie la référence de la publication de Emoto où différentes méthodes de démasquage d'antigène ont été testées et comparées : Emoto et al., J Histochem Cytochem. 2005. 53 (11) : 1311-21.

J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

Thank you for your inquiry.

The calculated molecular weight of NGF is 27kDa according to UniProt (http://www.uniprot.org/uniprot/P01138).

This molecular weight will be higher when the protein is phosphorylated and glycosylated. According to the SwissProt page there are multiple modifications in NGF.

We have customer feedback in form of an Abreview that will provide some more protocol information for you:

https://www.abcam.com/NGF-antibody-ab6199.html&tab=abreviews&intabreviewid=29302

There are also publications that might be helpful:

Zhao LH et al. Cornel iridoid glycoside improves memory ability and promotes neuronal survival in fimbria-fornix transected rats. Eur J Pharmacol 647:68-74 (2010). WB; Rat. PubMed: 20826142
Maezawa I et al. Rett syndrome astrocytes are abnormal and spread MeCP2 deficiency through gap junctions. J Neurosci 29:5051-61 (2009). WB; Mouse. PubMed: 19386901



I hope this information is helpful and wish you good luck with your experiments.

Thank you for your enquiry.

Here are answers to your questions:

1.So far we know heat mediated antigen retrieval has worked.

2. citrate buffer pH 6,0

3. Optimal dilution must be determined emperically, but 5ug/ml has been used succesfully.

4. Here is a link to published papers using ab6199:
https://www.abcam.com/NGF-antibody-ab6199-references.html

I hope this is helpful. Please contact us again if you have any further questions.

Thank you for your inquiry about a reference that was mentioned by a customer that submitted an Abreview (http://www.ncbi.nlm.nih.gov/pubmed/7815824)


Unfortunately, we do not have access to the specific article that was mentioned, nor do we have subscriptions to these journals. If you would like additional information regarding specific protocols or samples used, we would like to suggest contacting the authors of the article directly or ask your library to order the article for you.

For advice regarding our products and protocols, we are happy to assist you. Please do not hesitate to contact us if you would like additional information.

Merci pour votre réponse et ces précisions.

J'ai de nouveau regardé vos marquages et je validerais personnellement ces résultats. Les deux anticorps montrent un bon marquage spécifique et lescontroles négatifs avec l'isotype sont d'aucune ambiguité.

J'espère que ceci vous sera utile. Merci de nous avoir contactés. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.