Anti-NFYB/CBF-B 抗体 (ab111577)
Key features and details
- Rabbit polyclonal to NFYB/CBF-B
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NFYB/CBF-B antibody
NFYB/CBF-B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NFYB/CBF-B -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish
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免疫原
Recombinant fragment corresponding to Human NFYB/CBF-B aa 33-157.
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ポジティブ･コントロール
- HeLa nuclear cell lysate; HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab111577の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 23 kDa.
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| ICC/IF |
1/100 - 1/500.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 23 kDa. |
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ICC/IF
1/100 - 1/500. |
ターゲット情報
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機能
Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. -
配列類似性
Belongs to the NFYB/HAP3 subunit family. -
ドメイン
Can be divided into 3 domains: the weakly conserved A domain, the highly conserved B domain thought to be involved in subunit interaction and DNA binding, and the Glu-rich C domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 396205 Chicken
- Entrez Gene: 614382 Cow
- Entrez Gene: 4801 Human
- Entrez Gene: 18045 Mouse
- Entrez Gene: 100157587 Pig
- Entrez Gene: 25336 Rat
- Entrez Gene: 399126 Xenopus laevis
- Omim: 189904 Human
see all -
別名
- CAAT box DNA binding protein subunit B antibody
- CAAT box DNA-binding protein subunit B antibody
- CBF A antibody
see all
画像
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Western blot - Anti-NFYB/CBF-B antibody (ab111577)Anti-NFYB/CBF-B antibody (ab111577) at 1/1000 dilution + HeLa nuclear lysate at 30 µg
Predicted band size: 23 kDa
12% SDS-PAGE. -
Immunocytochemistry/ Immunofluorescence - Anti-NFYB/CBF-B antibody (ab111577)ab111577 at 1/500 dilution staining NFYB/CBF-B in paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab111577 は 2 報の論文で使用されています。
- Jia H et al. The neuroprotective effect of electro-acupuncture on cognitive recovery for patients with mild traumatic brain injury: A randomized controlled clinical trial. Medicine (Baltimore) 102:e32885 (2023). PubMed: 36820591
- Shi H et al. CEBPγ facilitates lamellipodia formation and cancer cell migration through CERS6 upregulation. Cancer Sci 112:2770-2780 (2021). PubMed: 33934437