Anti-NFYA 抗体 (ab6558)
Key features and details
- Rabbit polyclonal to NFYA
- Suitable for: ELISA, IP, EMSA, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-NFYA antibody
NFYA 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NFYA -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, IP, EMSA, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Monkey
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免疫原
Synthetic peptide corresponding to Human NFYA (N terminal) conjugated to keyhole limpet haemocyanin.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.42% Potassium phosphate -
Concentration information loading... -
精製度
Ion Exchange Chromatography -
特記事項（精製）
Anti-NF-Y (a) antibody was prepared from monospecific antiserum by a multi-step procedure that includes delipidation, salt fractionation and ion exchange chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6558の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
1/10000.
|
|
| IP |
Use at an assay dependent concentration.
|
|
| EMSA |
Use at an assay dependent concentration.
1-2 ul/assay. |
|
| WB | (1) |
1/1000.
|
| 特記事項 |
|---|
|
ELISA
1/10000. |
|
IP
Use at an assay dependent concentration. |
|
EMSA
Use at an assay dependent concentration. 1-2 ul/assay. |
|
WB
1/1000. |
ターゲット情報
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機能
Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. -
配列類似性
Belongs to the NFYA/HAP2 subunit family. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 4800 Human
- Entrez Gene: 18044 Mouse
- Omim: 189903 Human
- SwissProt: P23511 Human
- SwissProt: P23708 Mouse
- Unigene: 10441 Human
- Unigene: 4929 Mouse
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別名
- CAAT box DNA binding protein subunit A antibody
- CAAT box DNA-binding protein subunit A antibody
- CBF A antibody
see all
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab6558 は 7 報の論文で使用されています。
- Amirteimouri S et al. Epigenetic role of the nuclear factor NF-Y on ID gene family in endometrial tissues of women with endometriosis: a case control study. Reprod Biol Endocrinol 17:32 (2019). PubMed: 30876429
- Hiramoto M et al. A type 2 diabetes-associated SNP in KCNQ1 (rs163184) modulates the binding activity of the locus for Sp3 and Lsd1/Kdm1a, potentially affecting CDKN1C expression. Int J Mol Med 41:717-728 (2018). WB ; Rat . PubMed: 29207083
- Ma H et al. ZHX2 enhances the cytotoxicity of chemotherapeutic drugs in liver tumor cells by repressing MDR1 via interfering with NF-YA. Oncotarget 6:1049-63 (2015). IP, ChIP ; Human . PubMed: 25473899
- Lagadec C et al. The RNA-binding protein Musashi-1 regulates proteasome subunit expression in breast cancer- and glioma-initiating cells. Stem Cells 32:135-44 (2014). PubMed: 24022895
- Zhang Z et al. Immune mediators regulate CFTR expression through a bifunctional airway-selective enhancer. Mol Cell Biol 33:2843-53 (2013). PubMed: 23689137
- Hou Y et al. DNA Demethylation and USF Regulate the Meiosis-Specific Expression of the Mouse Miwi. PLoS Genet 8:e1002716 (2012). EMSA ; Mouse . PubMed: 22661915
- Grskovic M et al. Systematic identification of cis-regulatory sequences active in mouse and human embryonic stem cells. PLoS Genet 3:e145 (2007). EMSA ; Human . PubMed: 17784790