Anti-NFkB Inducing Kinase NIK 抗体 (ab7204)
Key features and details
- Rabbit polyclonal to NFkB Inducing Kinase NIK
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-NFkB Inducing Kinase NIK antibody
NFkB Inducing Kinase NIK 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NFkB Inducing Kinase NIK -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human NFkB Inducing Kinase NIK aa 795-947.
Database link: Q99558 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7204の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
|
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| WB | (2) |
1/2000.
|
| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 5 µg/ml. |
|
WB
1/2000. |
ターゲット情報
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機能
Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner. -
組織特異性
Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon. -
配列類似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization.
Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 9020 Human
- Omim: 604655 Human
- SwissProt: Q99558 Human
- Unigene: 404183 Human
- Unigene: 668927 Human
- Unigene: 735695 Human
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別名
- MAP3K14 antibody
- FTDCR1B antibody
- HS antibody
see all
画像
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Western blot - Anti-NFkB Inducing Kinase NIK antibody (ab7204)All lanes : Anti-NFkB Inducing Kinase NIK antibody (ab7204)
Lane 1 : macrophages lysate
Lane 2 : NIK transfected cell lysateWestern blot detection of NIK in 1, macrophage and 2, NIK transfected cells by anti-human NIK.
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Immunocytochemistry/ Immunofluorescence - Anti-NFkB Inducing Kinase NIK antibody (ab7204)ICC/IF image of ab7204 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7204, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab7204 は 14 報の論文で使用されています。
- Afsahi A et al. LCL161 enhances expansion and survival of engineered anti-tumor T cells but is restricted by death signaling. Front Immunol 14:1179827 (2023). PubMed: 37138866
- Xu Z et al. lncRNA PCGEM1 strengthens anti-inflammatory and lung protective effects of montelukast sodium in children with cough-variant asthma. Braz J Med Biol Res 53:e9271 (2020). PubMed: 32520202
- Fang C et al. ZFYVE21 is a complement-induced Rab5 effector that activates non-canonical NF-?B via phosphoinosotide remodeling of endosomes. Nat Commun 10:2247 (2019). PubMed: 31113953
- Wang X et al. Tunicamycin suppresses breast cancer cell growth and metastasis via regulation of the protein kinase B/nuclear factor-?B signaling pathway. Oncol Lett 15:4137-4142 (2018). PubMed: 29541178
- Yu R et al. Kruppel-like factor 4 inhibits non-small cell lung cancer cell growth and aggressiveness by stimulating transforming growth factor-ß1-meidated ERK/JNK/NF-?B signaling pathways. Tumour Biol 39:1010428317705574 (2017). PubMed: 28631556