Anti-NFIB / NF1B2 抗体 [EPR14122] (ab186738)

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Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14122] to NFIB / NF1B2
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase APC Carrier Free HRP PE

製品名

Anti-NFIB / NF1B2 antibody [EPR14122]
NFIB / NF1B2 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR14122] to NFIB / NF1B2
由来種

Rabbit
アプリケーション

適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details
種交差性

交差種: Mouse, Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- HeLa, rat C6, rat PC12 and mouse NIH 3T3 cell lysates; Human fetal brain lysate; Human breast and Mouse liver tissue; HeLa cells.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR14122
アイソタイプ

IgG
研究分野

Alternative Versions
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab186738の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
Flow Cyt (Intra)		Use at an assay dependent concentration.
WB		1/1000 - 1/10000. Detects a band of approximately 47,53,55 kDa (predicted molecular weight: 47 kDa).
IHC-P		1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF		1/100.
IP		1/30 - 1/50.

特記事項
Flow Cyt (Intra) Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Detects a band of approximately 47,53,55 kDa (predicted molecular weight: 47 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100.
IP 1/30 - 1/50.

機能

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
配列類似性

Belongs to the CTF/NF-I family.
Contains 1 CTF/NF-I DNA-binding domain.
細胞内局在

Nucleus.
Target information above from: UniProt accession O00712 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 4781 Human
- Entrez Gene: 18028 Mouse
- Entrez Gene: 29227 Rat
- Omim: 600728 Human
- SwissProt: O00712 Human
- SwissProt: P97863 Mouse
- Unigene: 644095 Human
- Unigene: 317947 Mouse
別名
- CCAAT Box Binding Transcription Factor antibody
- CCAAT-box-binding transcription factor antibody
- CTF antibody
- HMGIC/NFIB antibody
- NF-I/B antibody
- NF1-B antibody
- NF1B antibody
- NF1B2 antibody
- NFI-B antibody
- NFI-RED antibody
- Nfib antibody
- NFIB_HUMAN antibody
- NFIB2 antibody
- NFIB3 antibody
- Nuclear factor 1 B-type antibody
- Nuclear factor 1/B antibody
- Nuclear Factor 1B antibody
- Nuclear Factor I B antibody
- Nuclear factor I/B antibody
- TGGCA Binding Protein antibody
- TGGCA-binding protein antibody
- TRANSCRIPTION FACTOR NFIB antibody
see all

Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)

Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738) at 1/5000 dilution + Human fetal brain at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 47 kDa
Observed band size: 47 kDa
Flow Cytometry (Intracellular) - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)

ab186738 staining NFIB / NF1B2 in the human cell line HeLa (human cervix adenocarcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permiabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/70. A goat anti rabbit IgG (Alexa Fluor^® 488) at a dilution of 1/2000 was used as the secondary antibody.

Isoytype control: Rabbit monoclonal IgG (Black)

Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)

Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738) at 1/5000 dilution + HeLa cell lysate at 10 µg

Secondary
goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 47 kDa
Observed band size: 47, 53, 55 kDa why is the actual band size different from the predicted?
Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)

All lanes : Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738) at 1/1000 dilution

Lane 1 : C6 cell lysate
Lane 2 : PC-12 cell lysate
Lane 3 : NIH/3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 47 kDa
Observed band size: 47 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)

Immunohistochemical analysis of paraffin-embedded, Human breast tissue labeling NFIB / NF1B2 with ab186738 at a 1/500 dilution. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)

Immunohistochemical analysis of paraffin-embedded, mouse liver tissue labeling NFIB / NF1B2 with ab186738 at a 1/500 dilution. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)

Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling NFIB / NF1B2 with ab186738 at a 1/100 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®555) was used at a 1/200. In blue DAPI staining.
Immunoprecipitation - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)

Western blot analysis on immunoprecipitation from 1) Human fetal brain lysate and 2) PBS, labeling NFIB / NF1B2 using ab186738 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.
Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab186738 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab186738 は 19 報の論文で使用されています。

Zhang G et al. A Novel Molecular Classification Method for Glioblastoma Based on Tumor Cell Differentiation Trajectories. Stem Cells Int 2023:2826815 (2023). PubMed: 37964983
Zhang W et al. NFIB facilitates replication licensing by acting as a genome organizer. Nat Commun 14:5076 (2023). PubMed: 37604829
Parichha A et al. Dentate gyrus morphogenesis is regulated by β-catenin function in hem-derived fimbrial glia. Development 149:N/A (2022). PubMed: 36196585
Zhou L et al. Hepatocyte-Specific Knock-Out of Nfib Aggravates Hepatocellular Tumorigenesis via Enhancing Urea Cycle. Front Mol Biosci 9:875324 (2022). PubMed: 35655758
Huang S et al. Chimeric cerebral organoids reveal the essentials of neuronal and astrocytic APOE4 for Alzheimer's tau pathology. Signal Transduct Target Ther 7:176 (2022). PubMed: 35691989

View all Publications for this product

レビューと Q&A

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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Conjugation kits

Isotype control

Positive Controls

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

Certificate of Compliance

参考文献 (19)

レビューと Q&A