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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-NFAT4/NF-ATc3 抗体 [EPR24994-68] (ab283690)

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Western blot - Anti-NFAT4/NF-ATc3 antibody [EPR24994-68] (ab283690)
  • Western blot - Anti-NFAT4/NF-ATc3 antibody [EPR24994-68] (ab283690)
  • Anti-NFAT4/NF-ATc3 antibody [EPR24994-68] (ab283690)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24994-68] to NFAT4/NF-ATc3
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-NFAT4/NF-ATc3 antibody [EPR24994-68]
    NFAT4/NF-ATc3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR24994-68] to NFAT4/NF-ATc3

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt (Intra),ICC/IF,IHC-P or IP

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Jurkat, HeLa and HEK293T whole cell lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab283690の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Predicted molecular weight: 116 kDa.
特記事項
WB
1/1000. Predicted molecular weight: 116 kDa.
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF,IHC-P or IP.

ターゲット情報

  • 機能

    Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2.

  • 組織特異性

    Isoform 1 is predominantly expressed in thymus and is also found in peripheral blood leukocytes and kidney. Isoform 2 is predominantly expressed in skeletal muscle and is also found in thymus, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta and pancreas. Isoform 3 is expressed in thymus and kidney. Isoform 4 is expressed in thymus and skeletal muscle.

  • 配列類似性

    Contains 1 RHD (Rel-like) domain.

  • ドメイン

    Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.

  • 翻訳後修飾

    Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin.

  • 細胞内局在

    Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
  • Information by UniProt

  • 参照データベース

    • 別名

      • C80703 antibody
      • cytoplasmic 3 antibody
      • D8Ertd281e antibody
      • NF AT4 antibody
      • NF ATc3 antibody
      • NF-AT4 antibody
      • NF-ATc3 antibody
      • NFAC3_HUMAN antibody
      • NFAT4 antibody
      • NFATc3 antibody
      • NFATx antibody
      • Nuclear factor of activated T cells, cytoplasmic 3 antibody
      • nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3 antibody
      • Nuclear factor of activated T-cells antibody
      • T cell transcription factor NFAT4 antibody
      • T-cell transcription factor NFAT4 antibody
      see all

    画像

    • Western blot - Anti-NFAT4/NF-ATc3 antibody [EPR24994-68] (ab283690)
      Western blot - Anti-NFAT4/NF-ATc3 antibody [EPR24994-68] (ab283690)
      All lanes : Anti-NFAT4/NF-ATc3 antibody [EPR24994-68] (ab283690) at 1/1000 dilution

      Lane 1 : Wild-type HEK293T (human embryonic kidney epithelial cell) whole cell lysate
      Lane 2 : NFATC3 (NFAT4) knockout HEK293T (human embryonic kidney epithelial cell) whole cell lysate

      Lysates/proteins at 40 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (ab216773) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (ab216776) at 1/10000 dilution

      Predicted band size: 116 kDa
      Observed band size: 140 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 3% NFDM/TBST

      Lanes 1- 2: Merged signal (red and green). Green - ab283690 observed at 140 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

      ab283690 was shown to react with NFATC3 (NFAT4) in wild-type HEK293T cells in western blot. Loss of signal was observed when NFATC3 (NFAT4) knockout cell line ab267300 (knockout cell lysate ab258073) was used. Wild-type HEK293T and NFATC3 (NFAT4) knockout HEK293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab283690 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4°C overnight at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

      Lysates were made freshly and used in WB immediately to minimize protein degradation. 

    • Western blot - Anti-NFAT4/NF-ATc3 antibody [EPR24994-68] (ab283690)
      Western blot - Anti-NFAT4/NF-ATc3 antibody [EPR24994-68] (ab283690)
      All lanes : Anti-NFAT4/NF-ATc3 antibody [EPR24994-68] (ab283690) at 1/1000 dilution

      Lane 1 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
      Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 3 : HEK293T (human embryonic kidney epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 116 kDa
      Observed band size: 140-160 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 3% NFDM/TBST

      The molecular weight observed is consistent with what has been described in the literature (PMID: 31988379).

      Lysates were made freshly and used in WB immediately to minimize protein degradation.

      Exposure time: 3 minutes

    • Anti-NFAT4/NF-ATc3 antibody [EPR24994-68] (ab283690)
      Anti-NFAT4/NF-ATc3 antibody [EPR24994-68] (ab283690)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (1)

    ab283690 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab283690 は 1 報の論文で使用されています。

    • Zhang Y  et al. Angiotensin receptor-neprilysin inhibitor attenuates cardiac hypertrophy and improves diastolic dysfunction in a mouse model of heart failure with preserved ejection fraction. Clin Exp Pharmacol Physiol 49:848-857 (2022). PubMed: 35596518

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