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AB183023

Anti-NFAT2 (phospho S237) 抗体 [EPR2377(N)]

Anti-NFAT2 (phospho S237) antibody [EPR2377(N)]

4

(1 Review)

|

(7 Publications)

Rabbit Recombinant Monoclonal NFAT2 phospho S237 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications.

別名を表示する

NFAT2, NFATC, NFATC1, NF-ATc1, NFATc1, NFAT transcription complex cytosolic component, NF-ATc, NFATc

2 Images
Flow Cytometry (Intracellular) - Anti-NFAT2 (phospho S237) antibody [EPR2377(N)] (AB183023)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NFAT2 (phospho S237) antibody [EPR2377(N)] (AB183023)

Intracellular Flow Cytometry analysis of permealized 293 cells labeling NFAT2 (phospho S237) using ab183023 at 1/200 dilution (red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control : Rabbit monoclonal IgG (green).

Western blot - Anti-NFAT2 (phospho S237) antibody [EPR2377(N)] (AB183023)
  • WB

Supplier Data

Western blot - Anti-NFAT2 (phospho S237) antibody [EPR2377(N)] (AB183023)

All lanes:

Western blot - Anti-NFAT2 (phospho S237) antibody [EPR2377(N)] (ab183023) at 1/10000 dilution

Lane 1:

HEK-293 lysate treated with Ionomycin and TPA at 10 µg

Lane 2:

HEK-293 lysate treated with Ionomycin and TPA, then treated with alkaline phosphatase at 10 µg

Lane 3:

HEK-293 lysate untreated at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 101 kDa

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関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-NFAT2 (phospho S237) antibody [EPR2377(N)] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR2377(N)

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/200", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NFAT2 also known as NFATc1 is a transcription factor with a mass of approximately 100 kDa. It belongs to the Nuclear Factor of Activated T-cells (NFAT) family which plays significant roles in gene expression regulation. NFAT2 is expressed in T-cells B-cells and cardiac muscle tissues. It is activated through dephosphorylation by the enzyme calcineurin allowing it to translocate into the nucleus. NFAT2 binds to specific DNA motifs to regulate the transcription of target genes impacting various cellular processes.
Biological function summary

NFAT2 plays a significant role in the regulation of immune responses and cardiac hypertrophy. It functions as part of a complex with AP-1 (Activator Protein-1) during T-cell activation integrating signals necessary for cytokine production and immune cell differentiation. In cardiac cells NFAT2 influences the expression of genes involved in hypertrophic growth. NFAT2 contributes to the development and function of the immune system by modulating critical immune pathways and cellular proliferation.

Pathways

NFAT2 serves a vital function in the calcineurin-NFAT signaling pathway. This pathway is important for T-cell activation and is triggered by increased intracellular calcium levels leading to dephosphorylation of NFAT2. The dephosphorylated NFAT2 cooperates with proteins such as AP-1 to induce expression of cytokines like interleukin-2. NFAT2 is also linked with the MAPK signaling pathway which affects cellular proliferation and differentiation. Together these pathways highlight the integral role of NFAT2 in immune system function and cardiac development.

NFAT2 has strong connections to autoimmune diseases and cardiac hypertrophy. Aberrations in NFAT2 activity and regulation are associated with autoimmune conditions due to its central role in immune response modulation. In cardiac hypertrophy heightened NFAT2 activity may lead to pathological enlargement of heart tissue. The protein has interactions with calcineurin which also influences these conditions. Understanding these relationships helps in exploring therapeutic targets for managing immune-related diseases and cardiac dysfunctions.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells (PubMed : 10358178). Required for osteoclastogenesis and regulates many genes important for osteoclast differentiation and function (By similarity).
See full target information NFATC1 pS237
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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 16:336 PubMed40597262

2025

VEGF-B-mediated myofiber types involved in high-fat diet-induced hyperglycemia through PKA-NFATs signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Liu-Liu Shi,Yu-Ting Sun,Jia-Nan Sun,Jing Yue,Wei Chen,Kun Meng,Long Chen,Chang-Qing Hu,Rui Chen,Dong-Sheng Sun,Cheng-Bao Xu,Wei Yuan,Xin-Li Li,Dan Zhao,Yan Wu,Shi-Bing Xi,Xiao-Ying Zhao,Jun-Ming Tang

Journal for immunotherapy of cancer 13: PubMed40447319

2025

Cbl-b inhibitor NX-1607 activates MAPK/ERK signaling pathway and enhances T-cell activation.

Applications

Unspecified application

Species

Unspecified reactive species

Wenting Zhu,Shan Lu,Li Jia,Benjin Liu,Shanshan Song,Xubin Bao,Ting Yu,Yongliang Zhang,Zehong Miao,Jinxue He

Nature communications 14:8491 PubMed38123592

2023

Cyclosporine A-resistant CAR-T cells mediate antitumour immunity in the presence of allogeneic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yixi Zhang,Hongyu Fang,Guocan Wang,Guangxun Yuan,Ruoyu Dong,Jijun Luo,Yu Lyu,Yajie Wang,Peng Li,Chun Zhou,Weiwei Yin,Haowen Xiao,Jie Sun,Xun Zeng

Molecular cancer therapeutics 22:913-925 PubMed37196158

2023

Preclinical Evaluation of 9MW2821, a Site-Specific Monomethyl Auristatin E-based Antibody-Drug Conjugate for Treatment of Nectin-4-expressing Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhou,Peng Fang,Dongan Yu,Hongyuan Ren,Meng You,Long Yin,Fei Mei,Huikai Zhu,Zhenzhen Wang,Hui Xu,Yuxia Cao,Xiaowei Sun,Xiaohong Xu,Jianjun Bi,Jin Wang,Lanping Ma,Xin Wang,Lin Chen,Yongliang Zhang,Xiaowei Cen,Xi Zhu,Liguang Lou,Datao Liu,Xiaoding Tan,Jinliang Yang,Tao Meng,Jingkang Shen

Stem cells translational medicine 12:307-321 PubMed37010483

2023

WTAP-Mediated m6A RNA Methylation Regulates the Differentiation of Bone Marrow Mesenchymal Stem Cells via the miR-29b-3p/HDAC4 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jincheng Liu,Yunhao You,Zhenqian Sun,Lu Zhang,Xiang Li,Zihan Dai,Jinlong Ma,Yunzhen Chen,Guangjun Jiao

BioMed research international 2019:6712536 PubMed31183372

2019

TRPV1 Induced Apoptosis of Colorectal Cancer Cells by Activating Calcineurin-NFAT2-p53 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Nengyi Hou,Xuelai He,Yuhui Yang,Junwen Fu,Wei Zhang,Zhiyi Guo,Yang Hu,Liqin Liang,Wei Xie,Haibo Xiong,Kang Wang,Minghui Pang

PloS one 12:e0181608 PubMed28750002

2017

DNA-PKcs controls calcineurin mediated IL-2 production in T lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Ara Kim Wiese,Marie Schluterman Burdine,Richard H Turnage,Alan J Tackett,Lyle J Burdine
View all publications
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