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AB180510

Anti-NFAT2 (phospho S233) 抗体 [EPR2376(N)]

Anti-NFAT2 (phospho S233) antibody [EPR2376(N)]

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(1 Publication)

Rabbit Recombinant Monoclonal NFAT2 phospho S233 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

別名を表示する

NFAT2, NFATC, NFATC1, NF-ATc1, NFATc1, NFAT transcription complex cytosolic component, NF-ATc, NFATc

1 Images
Western blot - Anti-NFAT2 (phospho S233) antibody [EPR2376(N)] (AB180510)
  • WB

Supplier Data

Western blot - Anti-NFAT2 (phospho S233) antibody [EPR2376(N)] (AB180510)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-NFAT2 (phospho S233) antibody [EPR2376(N)] (ab180510) at 1/20000 dilution

Lane 1:

Jurkat cell lysate + Calyculin A at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 10 kDa,101 kDa,14 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-NFAT2 (phospho S233) antibody [EPR2376(N)] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR2376(N)

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

ab180510 recognizes 7 isoforms

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製度
Tissue culture supernatant
バッファー組成
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NFAT2 also known as NFATc1 is a transcription factor with a mass of approximately 100 kDa. It belongs to the Nuclear Factor of Activated T-cells (NFAT) family which plays significant roles in gene expression regulation. NFAT2 is expressed in T-cells B-cells and cardiac muscle tissues. It is activated through dephosphorylation by the enzyme calcineurin allowing it to translocate into the nucleus. NFAT2 binds to specific DNA motifs to regulate the transcription of target genes impacting various cellular processes.
Biological function summary

NFAT2 plays a significant role in the regulation of immune responses and cardiac hypertrophy. It functions as part of a complex with AP-1 (Activator Protein-1) during T-cell activation integrating signals necessary for cytokine production and immune cell differentiation. In cardiac cells NFAT2 influences the expression of genes involved in hypertrophic growth. NFAT2 contributes to the development and function of the immune system by modulating critical immune pathways and cellular proliferation.

Pathways

NFAT2 serves a vital function in the calcineurin-NFAT signaling pathway. This pathway is important for T-cell activation and is triggered by increased intracellular calcium levels leading to dephosphorylation of NFAT2. The dephosphorylated NFAT2 cooperates with proteins such as AP-1 to induce expression of cytokines like interleukin-2. NFAT2 is also linked with the MAPK signaling pathway which affects cellular proliferation and differentiation. Together these pathways highlight the integral role of NFAT2 in immune system function and cardiac development.

NFAT2 has strong connections to autoimmune diseases and cardiac hypertrophy. Aberrations in NFAT2 activity and regulation are associated with autoimmune conditions due to its central role in immune response modulation. In cardiac hypertrophy heightened NFAT2 activity may lead to pathological enlargement of heart tissue. The protein has interactions with calcineurin which also influences these conditions. Understanding these relationships helps in exploring therapeutic targets for managing immune-related diseases and cardiac dysfunctions.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells (PubMed : 10358178). Required for osteoclastogenesis and regulates many genes important for osteoclast differentiation and function (By similarity).
See full target information NFATC1 pS233
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Neural regeneration research 19:1618-1624 PubMed38051907

2023

Melatonin improves synapse development by PI3K/Akt signaling in a mouse model of autism spectrum disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Luyi Wang,Man Xu,Yan Wang,Feifei Wang,Jing Deng,Xiaoya Wang,Yu Zhao,Ailing Liao,Feng Yang,Shali Wang,Yingbo Li
View all publications
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代替製品

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AB240262

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組合せ製品

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Abcam product promise

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