AB25916

Anti-NFAT2 抗体

Name: Anti-NFAT2 抗体 (ab25916) | Abcam
Brand: Abcam Limited
SKU: AB25916
Price: 77000 JPY
Availability: InStock
Rating: 4 (11 reviews)

Anti-NFAT2 antibody

(11 Reviews)

(82 Publications)

Anti-NFAT2 antibody (ab25916) is a rabbit polyclonal antibody detecting NFAT2 in Western Blot, ICC/IF. Suitable for Chimpanzee, Human, Mouse.

- Over 50 publications
- Trusted since 2005

別名を表示する

NFAT2, NFATC, NFATC1, NF-ATc1, NFATc1, NFAT transcription complex cytosolic component, NF-ATc, NFATc

3 Images

Supplier Data

Western blot - Anti-NFAT2 antibody (AB25916)

All lanes:

Western blot - Anti-NFAT2 antibody (ab25916) at 1 mg/mL

Lane 1:

Ramos (human Burkitt's lymphoma cell line) whole cell lysate

Lane 2:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

Predicted band size: 101 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-NFAT2 antibody (AB25916)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NFAT2 antibody (AB25916)

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells stained for NFAT2 (green) using ab25916 at 1/500 dilution in ICC/IF.

The nuclear counterstain is DAPI (blue). Alpha-Tubulin is stained with Dylight 550 (red).

Unknown

Western blot - Anti-NFAT2 antibody (AB25916)

Western blot analysis of NFATc1 in Ramos cell lysate. Lane 1. Without blocking peptide. Lane 2. With blocking peptide.

All lanes:

Western blot - Anti-NFAT2 antibody (ab25916)

Predicted band size: 101 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human, Chimpanzee

アプリケーション

WB, ICC/IF

applications

免疫原

Synthetic Peptide within Human NFATC1 aa 250-300. The exact immunogen used to generate this antibody is proprietary information.

O95644

特異性

The amino acid sequence used is 100% identical in human NFATc1A, B, C and E isoforms. The NFATc1 antibody may detect multiple isoforms of different sizes depending upon the cell lines.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "Predicted molecular weight varies with isoform.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/20", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "Predicted molecular weight varies with isoform.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/20", "ICCIF-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Anti-NFAT2 antibody (ab25916) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Chimpanzee, Human, Mouse samples.

Trusted by the scientific community
Anti-NFAT2 (ab25916) was first used in a scientific publication in 2005 and has been cited over 50 times in peer-reviewed journals.

Reviewed by scientists
Anti-NFAT2 (ab25916) has over 10 independent reviews from customers.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

バッファー組成

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NFAT2 also known as NFATc1 is a transcription factor with a mass of approximately 100 kDa. It belongs to the Nuclear Factor of Activated T-cells (NFAT) family which plays significant roles in gene expression regulation. NFAT2 is expressed in T-cells B-cells and cardiac muscle tissues. It is activated through dephosphorylation by the enzyme calcineurin allowing it to translocate into the nucleus. NFAT2 binds to specific DNA motifs to regulate the transcription of target genes impacting various cellular processes.

Biological function summary

NFAT2 plays a significant role in the regulation of immune responses and cardiac hypertrophy. It functions as part of a complex with AP-1 (Activator Protein-1) during T-cell activation integrating signals necessary for cytokine production and immune cell differentiation. In cardiac cells NFAT2 influences the expression of genes involved in hypertrophic growth. NFAT2 contributes to the development and function of the immune system by modulating critical immune pathways and cellular proliferation.

Pathways

NFAT2 serves a vital function in the calcineurin-NFAT signaling pathway. This pathway is important for T-cell activation and is triggered by increased intracellular calcium levels leading to dephosphorylation of NFAT2. The dephosphorylated NFAT2 cooperates with proteins such as AP-1 to induce expression of cytokines like interleukin-2. NFAT2 is also linked with the MAPK signaling pathway which affects cellular proliferation and differentiation. Together these pathways highlight the integral role of NFAT2 in immune system function and cardiac development.

NFAT2 has strong connections to autoimmune diseases and cardiac hypertrophy. Aberrations in NFAT2 activity and regulation are associated with autoimmune conditions due to its central role in immune response modulation. In cardiac hypertrophy heightened NFAT2 activity may lead to pathological enlargement of heart tissue. The protein has interactions with calcineurin which also influences these conditions. Understanding these relationships helps in exploring therapeutic targets for managing immune-related diseases and cardiac dysfunctions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells (PubMed : 10358178). Required for osteoclastogenesis and regulates many genes important for osteoclast differentiation and function (By similarity).

See full target information NFATC1

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文献 (82)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 20:713 PubMed40722101

2025

Licochalcone D inhibits osteoclast differentiation and postmenopausal osteoporosis by inactivating the NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyi Shen,Qian Zhang,Jingjing Ding,Jun Zhou,Sasa Tan,Xianzhen Feng,Zhongqing Xu,Fei Hua

Stem cell research & therapy 16:336 PubMed40597262

2025

VEGF-B-mediated myofiber types involved in high-fat diet-induced hyperglycemia through PKA-NFATs signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Liu-Liu Shi,Yu-Ting Sun,Jia-Nan Sun,Jing Yue,Wei Chen,Kun Meng,Long Chen,Chang-Qing Hu,Rui Chen,Dong-Sheng Sun,Cheng-Bao Xu,Wei Yuan,Xin-Li Li,Dan Zhao,Yan Wu,Shi-Bing Xi,Xiao-Ying Zhao,Jun-Ming Tang

Drug design, development and therapy 19:4689-4715 PubMed40486125

2025

Lobetyolin Suppressed Osteoclastogenesis and Alleviated Bone Loss in Ovariectomy-Induced Osteoporosis via Hindering p50/p65 Nuclear Translocation and Downstream NFATc1/c-Fos Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Chunmei Xiu,Hua Luo,Weixing Huang,Shaohua Fan,Chiting Yuan,Jiangjie Chen,Chenghao Xu,Can Yao,Dun Hong,Liwei Zhang

Journal of nanobiotechnology 23:348 PubMed40369573

2025

Engineered bone-targeting apoptotic vesicles as a minimally invasive nanotherapy for heterotopic ossification.

Applications

Unspecified application

Species

Unspecified reactive species

Like Tang,Yuchen Wang,Shihua Mao,Zhou Yu,Yitong Chen,Xiaoqiao Xu,Wenjin Cai,Kaichen Lai,Guoli Yang,Tingben Huang

Journal of molecular endocrinology 74: PubMed40215127

2025

Identification of anti-resorptive GPCRs by high-content imaging in human osteoclasts.

Applications

Unspecified application

Species

Unspecified reactive species

Maria L Price,Rachael A Wyatt,Joao Correia,Zakia Areej,Maisie Hinds,Ana Crastin,Rowan S Hardy,Morten Frost,Caroline M Gorvin

Inflammation 48:3446-3457 PubMed40053057

2025

USP5 Deletion Inhibits KD Serum Induced-Human Coronary Artery Endothelial Cell Dysfunction by Regulating the NFATC1/TLR4-Mediated NF-κB Signaling Pathway in Kawasaki Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Lidan Yao,Yupeng Lai,Heng Li,Sihan Chen,Xianjia Yu,Ni Zhou,Dandan Lang

The Journal of clinical investigation 135: PubMed39585787

2024

Elevated microRNA-187 causes cardiac endothelial dysplasia to promote congenital heart disease through inhibition of NIPBL.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Li,Zizheng Tan,Hongdou Li,Xiaoying Yao,Chuyue Peng,Yue Qi,Bo Wu,Tongjin Zhao,Chentao Li,Jianfeng Shen,Hongyan Wang

The Kaohsiung journal of medical sciences 40:1057-1067 PubMed39548856

2024

Nuciferine inhibits osteoclast formation through suppressing glycolysis metabolic programming and ROS production.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Hui Guo,Guan-Qi Zhen,Feng Wu,Yun-Peng Lv,Jing-Long Yan,Jia-Ning Zu,Cheng-Chao Song

Journal of orthopaedic translation 48:190-203 PubMed39280633

2024

METTL14-mediated HOXA5 mA modification alleviates osteoporosis via promoting WNK1 transcription to suppress NLRP3-dependent macrophage pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Tang,Yuxuan Du,Zejiu Tan,Dongpeng Li,Jiang Xie

Nature communications 15:7324 PubMed39183203

2024

Multi-omics profiling of retinal pigment epithelium reveals enhancer-driven activation of RANK-NFATc1 signaling in traumatic proliferative vitreoretinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyu Liao,Xu Zhu,Yumei Lu,Xiaoping Yi,Youhui Hu,Yumeng Zhao,Zhisheng Ye,Xu Guo,Minghui Liang,Xin Jin,Hong Zhang,Xiaohong Wang,Ziming Zhao,Yupeng Chen,Hua Yan

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NFAT2 抗体

Anti-NFAT2 antibody

Western blot - Anti-NFAT2 antibody (AB25916)

Immunocytochemistry/ Immunofluorescence - Anti-NFAT2 antibody (AB25916)

Western blot - Anti-NFAT2 antibody (AB25916)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (82)

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Abcam product promise