AB244310

Anti-NFAT1 抗体

Name: Anti-NFAT1 抗体 (ab244310) | Abcam
Brand: Abcam Limited
SKU: AB244310
Price: 90000 JPY
Availability: InStock

Anti-NFAT1 antibody

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Rabbit Polyclonal NFAT1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NFATC2 aa 1-200.

別名を表示する

NFAT1, NFATP, NFATC2, NF-ATc2, NFATc2, NFAT pre-existing subunit, T-cell transcription factor NFAT1, NF-ATp

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-NFAT1 antibody (AB244310)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NFAT1 antibody (AB244310)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for NFAT1 (green) using ab244310 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT1 antibody (AB244310)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT1 antibody (AB244310)

Formalin-fixed, paraffin-embedded human lymph node tissue stained for NFAT1 using ab244310 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT1 antibody (AB244310)

Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for NFAT1 using ab244310 at 1/50 dilution in immunohistochemical analysis.

No positivity in myocytes, as expected.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT1 antibody (AB244310)

Formalin-fixed, paraffin-embedded human duodenum tissue stained for NFAT1 using ab244310 at 1/50 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-NFAT1 antibody (AB244310)

All lanes:

Western blot - Anti-NFAT1 antibody (ab244310) at 0.4 µg/mL

All lanes:

Human cell line CACO-2

Predicted band size: 100 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IHC-P, ICC/IF, WB

applications

免疫原

Recombinant Fragment Protein within Human NFATC2 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q13469

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代替製品

Primary Antibodies

AB198748

Anti-NFAT1 antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT1 antibody - N-terminal (AB198748)

Primary Antibodies

AB200819

Anti-NFAT1 (phospho S54) antibody

Western blot - Anti-NFAT1 (phospho S54) antibody (AB200819)

Primary Antibodies

AB2722

Anti-NFAT1 antibody [25A10.D6.D2]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT1 antibody [25A10.D6.D2] (AB2722)

組合せ製品

Assay Kits

AB300322

Human NFAT1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human NFAT1 ELISA Kit (AB300322)

Primary Antibodies

AB2796

Anti-NFAT2 antibody [7A6]

BOND RX™ Validated|KO Validated|Trial Size

Flow Cytometry (Intracellular) - Anti-NFAT2 antibody [7A6] (AB2796)

Primary Antibodies

AB32537

Anti-ERK1 antibody [Y72]

Flow Cytometry (Intracellular) - Anti-ERK1 antibody [Y72] (AB32537)

Primary Antibodies

AB181602

Anti-GAPDH antibody [EPR16891] - Loading Control

RabMAb|Recombinant|Lab Essentials|20ul selling size

Primary Antibodies

AB52903

Anti-SMAD3 (pS423/425) + SMAD5 (pS463/465) + SMAD1 (pS463/465) + SMAD2 (pS465/467) antibody [EP823Y]

20ul selling size|Recombinant|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAD3 (pS423/425) + SMAD5 (pS463/465 ) + SMAD1 (pS463/465) + SMAD2 (pS465/467) antibody [EP823Y] (AB52903)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NFAT1 also known as NFATc2 is a transcription factor involved in gene regulation with a molecular weight of approximately 100 kDa. It belongs to the NFAT (nuclear factor of activated T-cells) family and is expressed in a variety of tissues including the immune system and the nervous system. NFAT1 plays an essential role in activating specific gene expressions by translocating into the nucleus upon cell stimulation. Researchers frequently use NFAT molecular weight to confirm its presence in protein analyses such as NFAT western blot techniques.

Biological function summary

NFAT1 influences immune response and development. It acts as part of a large complex with other transcription factors to bind DNA and regulate transcription of cytokines and other critical immune molecules. In activated T-cells NFAT1 controls the expression of genes important for immune function including interleukins. In neuronal tissues it contributes to neural growth and survival highlighting its multifunctional nature in different cell types.

Pathways

NFAT1 integrates into the calcineurin signaling pathway and plays a significant role in T-cell activation. It acts downstream of the calcium signaling cascade which leads to dephosphorylation and activation by the phosphatase calcineurin. NFAT1 works closely with proteins like AP-1 and GATA to facilitate the transcription of immune-related genes. These interactions highlight NFAT1's importance in immune signaling pathways which modulate immune surveillance and homeostasis.

NFAT1 associates with autoimmune diseases and certain cancers. In autoimmune conditions such as rheumatoid arthritis aberrant NFAT1 function leads to excessive cytokine production exacerbating inflammation. The protein also links to cancer development particularly in lymphoid malignancies where dysregulated NFAT1 activity contributes to uncontrolled cell proliferation. It interacts with oncogenic kinases and other dysregulated proteins in these disorders influencing both their progression and possible therapeutic targeting.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF (PubMed : 15790681). Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway (PubMed : 21871017). Is involved in the negative regulation of chondrogenesis (PubMed : 35789258). Recruited by AKAP5 to ORAI1 pore-forming subunit of CRAC channels in Ca(2+) signaling microdomains where store-operated Ca(2+) influx is coupled to calmodulin and calcineurin signaling and activation of NFAT-dependent transcriptional responses.

See full target information NFATC2

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Abcam product promise

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