Name: Anti-NFAT1 antibody [25A10.D6.D2]
SKU: ab2722
Rating: 5 (7 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT1 antibody [25A10.D6.D2] (ab2722)

Key features and details

Mouse monoclonal [25A10.D6.D2] to NFAT1
Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
Reacts with: Human
Isotype: IgG1

製品名

Anti-NFAT1 antibody [25A10.D6.D2]
NFAT1 一次抗体製品一覧
製品の詳細

Mouse monoclonal [25A10.D6.D2] to NFAT1
由来種

Mouse
特異性

Ab2722 detects nuclear factor of activated T-cells (NFAT) from mouse, rat and human tissues (endogenously expressed). This antibody does not cross react with NFAT2 (NFATc, NFATc1). This antibody detects both forms NFAT1 - a ~140 kDa protein representing phosphorylated NFAT1 in resting immune cells, and a ~120 kDa protein in stimulated cells that represents fully-dephosphorylated NFAT1.
アプリケーション

適用あり: Flow Cyt, WB, IHC-P, ICC/IFmore details
種交差性

交差種: Human
免疫原

Synthetic peptide corresponding to Mouse NFAT1 aa 50-150.
Database link: Q60591

Run BLAST with Run BLAST with
ポジティブ･コントロール
- WB: resting immune cells and ionomycin stimulated immune cells (see Shaw et al reference: "1uM for T cells and B cells, 10uM for macrophages,and 0.3uM for mast cells. Treatment was for 20 min.")
特記事項

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファー

Preservative: 0.05% Sodium azide
Constituent: PBS
Concentration information loading...
精製度

Protein G purified
ポリ/モノ

モノクローナル
クローン名

25A10.D6.D2
アイソタイプ

IgG1
研究分野

ChIP Related Products
- ChIP Kit (ab500)
Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab2722の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
Flow Cyt		Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB	(4)	Use a concentration of 1 µg/ml. Detects a band of approximately 120, 140 kDa (predicted molecular weight: 115 kDa).
IHC-P	(1)	1/50 - 1/200.
ICC/IF		1/100 - 1/1000.

特記事項
Flow Cyt Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 120, 140 kDa (predicted molecular weight: 115 kDa).
IHC-P 1/50 - 1/200.
ICC/IF 1/100 - 1/1000.

機能

Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF.
組織特異性

Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas.
配列類似性

Contains 1 RHD (Rel-like) domain.
ドメイン

Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
翻訳後修飾

In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated; which is required for full transcriptional activity.
細胞内局在

Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
Target information above from: UniProt accession Q13469 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 4773 Human
- Omim: 600490 Human
- SwissProt: Q13469 Human
- Unigene: 713650 Human
別名
- AI607462 antibody
- cytoplasmic 2 antibody
- KIAA0611 antibody
- NF ATc2 antibody
- NF ATp antibody
- NF-ATc2 antibody
- NF-ATp antibody
- NFAC2_HUMAN antibody
- NFAT 1 antibody
- NFAT pre existing subunit antibody
- NFAT pre-existing subunit antibody
- NFAT transcription complex, preexisting component antibody
- NFAT1 antibody
- NFAT1-D antibody
- NFATc2 antibody
- NFATp antibody
- Nuclear factor of activated T cells cytoplasmic 2 antibody
- Nuclear factor of activated T cells cytoplasmic calcineurin dependent 2 antibody
- Nuclear factor of activated T cells pre-existing component antibody
- Nuclear factor of activated T cells, preexisting component antibody
- Nuclear factor of activated T-cells antibody
- Preexisting nuclear factor of activated T cells 2 antibody
- T cell transcription factor NFAT 1 antibody
- T-cell transcription factor NFAT1 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT1 antibody [25A10.D6.D2] (ab2722)

Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human spleen tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing NFATc2 ab2722 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunocytochemistry/ Immunofluorescence - Anti-NFAT1 antibody [25A10.D6.D2] (ab2722)

Immunocytochemistry/Immunofluorescence analysis of NFAT1 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA for 15 minutes at room temperature. Cells were left untreated (left panel) or treated with 1uM staurosporine (right panel) for 3 hours and incubated with ab2722 (1:100) for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488 conjugated goat anti-mouse IgG secondary antibody (1:400) for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.
Western blot - Anti-NFAT1 antibody [25A10.D6.D2] (ab2722)

Anti-NFAT1 antibody [25A10.D6.D2] (ab2722) at 1 µg/ml + Human spleen tissue lysate - total protein (ab29699) at 10 µg

Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution ( )

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 115 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: 62 kDa. We are unsure as to the identity of these extra bands.

Exposure time: 4 minutes

NFAT1 contains an exstensive number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Flow Cytometry - Anti-NFAT1 antibody [25A10.D6.D2] (ab2722)

Overlay histogram showing Jurkat cells stained with ab2722 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2722, 2µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT1 antibody [25A10.D6.D2] (ab2722)

ab2722 (4µg/ml) staining NFAT in human tonsil, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear and weak cytoplasmic staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Immunocytochemistry/ Immunofluorescence - Anti-NFAT1 antibody [25A10.D6.D2] (ab2722)

Immunocytochemistry/Immunofluorescence analysis of NFAT1 (green) shows staining in HeLa cells. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were inbuated without (control) or with ab2722 (1:20) over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
Immunocytochemistry/ Immunofluorescence - Anti-NFAT1 antibody [25A10.D6.D2] (ab2722)

Immunocytochemistry/Immunofluorescence analysis of NFAT1 (green) shows staining in MCF-7 cells. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were inbuated without (control) or with ab2722 (1:20) over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
Immunocytochemistry/ Immunofluorescence - Anti-NFAT1 antibody [25A10.D6.D2] (ab2722)

Immunocytochemistry/Immunofluorescence analysis of NFAT1 (green) shows staining in U251 Cells. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were inbuated without (control) or with ab2722 (1:20) over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT1 antibody [25A10.D6.D2] (ab2722)

Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing NFATc2 ab2722 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT1 antibody [25A10.D6.D2] (ab2722)

Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human tonsil tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing NFATc2 ab2722 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab2722 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab2722 は 53 報の論文で使用されています。

Lao M et al. Regulator of calcineurin 1 gene isoform 4 in pancreatic ductal adenocarcinoma regulates the progression of tumor cells. Oncogene 40:3136-3151 (2021). PubMed: 33824473
Cui C et al. Propofol maintains Th17/Treg cell balance and reduces inflammation in rats with traumatic brain injury via the miR-145-3p/NFATc2/NF-?B axis. Int J Mol Med 48:N/A (2021). PubMed: 34036377
Gajewska KA et al. Nuclear transporter Importin-13 plays a key role in the oxidative stress transcriptional response. Nat Commun 12:5904 (2021). PubMed: 34625540
Zhang Y et al. SNPs for Genes Encoding the Mitochondrial Proteins Sirtuin3 and Uncoupling Protein 2 Are Associated With Disease Severity, Type 2 Diabetes, and Outcomes in Patients With Pulmonary Arterial Hypertension and This Is Recapitulated in a New Mouse Model Lacking Both Genes. J Am Heart Assoc 10:e020451 (2021). PubMed: 34719264
Wu X et al. Inhibition of Lipopolysaccharide-Induced Inflammatory Bone Loss by Saikosaponin D is Associated with Regulation of the RANKL/RANK Pathway. Drug Des Devel Ther 15:4741-4757 (2021). PubMed: 34848946

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-10 of 23 Abreviews or Q&A

Application

Western blot

Sample

Mouse Tissue lysate - whole (Heart)

Gel Running Conditions

Reduced Denaturing (4-12%)

Loading amount

30 µg

Specification

Heart

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Aug 18 2020

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Heart)

Permeabilization

Yes - Tween-20

Specification

Heart

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Aug 11 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (PDAC)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate Ph:6.0

Permeabilization

Specification

PDAC

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 12 2019

Application

ChIP

Sample

Mouse Cell lysate - nuclear (CD4 T cells)

Negative control

NFAT1 ChIPseq - untreated cells. Gal4 ChIPseq - IgG control, cells treated for one hour with PMA plus ionomycin

Specification

CD4 T cells

Detection step

Other

Type

Native ChIP (N-ChIP)

Positive control

NFAT1 ChIPseq - cells treated for one hour with PMA plus ionomycin

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Giuliana Mognol

Verified customer

投稿 Jun 08 2017

Application

Western blot

Loading amount

20 µg

Gel Running Conditions

Reduced Denaturing (10%)

Sample

Human Purified protein (Human primary CD4 T cells)

Specification

Human primary CD4 T cells

Treatment

TCR activated using anti-CD3/CD28 for 3,10 and 30min

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Marco Craveiro

Verified customer

投稿 Jul 23 2013

Question

Would this antibody work for ChIP? Do you recommend other NFAT abs for ChIP?

Abcam community

Verified customer

Asked on Nov 26 2012

Answer

Thank you very much for your interest in ab92490.

To our knowledge, this product nor any of our other anti-NFAT products, have not been tested in ChIP. However by participating in our AbTrial program, you can now use our products in an untested application or species without financial risk.

Simply follow these easy steps below to take part in our AbTrial Program:

1. Reply to this e-mail to let me know that you would like to proceed and test this product.

2. I will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at regular price.

4. https://www.abcam.com/abreviews with your results. Note your AbTrial discount code in the additional notes section.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

More information including the terms and conditions about this program may be found at: https://www.abcam.com/AbTrial

Abcam Scientific Support

Answered on Nov 26 2012

Question

vielen Dank für Ihre Kulanz!

Der abgelaufene Discount Code lautet xxxxx.

Der Abreview ist für den Anti- NFAT1 (ab2722), Anonymous Abreview submitted on 11 April 2012.

Abcam community

Verified customer

Asked on Nov 26 2012

Answer

Vielen dank für die Bestätigung des Codes und des Abreviews.

Ihr neuer Code lautet: xxxxxxx.

Der Code ist über einen Wert von €410.00 und gültig bis zum 26/03/2013.

Abcam Scientific Support

Answered on Nov 26 2012

Question

Using spleen tissue but the band is smeary unlike the clear band shown in the image on the datasheet. How was this image made? How was the spleen lysate prepared?

Abcam community

Verified customer

Asked on Aug 03 2012

Answer

Thank you for taking the time to complete our recent phone survey about your experiences with Abcam.

I'd like to follow-up on this issue since the problem has not been resolved at this point. If the protocol suggestionswere not helpful, then I would be happy to send a replacement antibody or issue a credit or refund.

Please let me know if there is anything else that we can do for you. I look forward to hearing from you and resolving this as soon as possible.

Have a nice day.

Abcam Scientific Support

Answered on Aug 03 2012

Question

I have performed the western blotting with your antibody ab2722 as follows:

1) I did not check the membran by Ponceau staining

2) I did not check the gel by coomassie staining, but the protein ladder that I used was, especially within the high molecular band range, not blotted well compared to the low molecular bands

3) Loading buffer (NuPAGEÒ LDS sample buffer) and all further reagent are from Invitrogen, 6 µg of protein were loaded per lane

4) Transfer: 20 V ( this is the maximum for the used Xcell II Blot Modul (Invitrogen) for 4,5 h

5) Transfer buffer: NuPAGEÒ transfer buffer from Invitrogen + 0.1% SDS + 5% Methanol

6) PVDF membrane (pre-activated in methanol)

7) Xcell II Blot Modul (Invitrogen), wet blotting

Usually, for proteins smaller than 85 kDa, I perform the blotting just for 1,5 hours and it works pretty well. Furthermore, all of the ladder bands are visible quite good in this case.

It would be very helpfull if you could provide the exact protocol from the testing of this antibody as indicated in the antibody datasheet!

With kind regards,

Abcam community

Verified customer

Asked on Jul 03 2012

Answer

Thank you for your patience.

The laboratory has got back to me - however unfortunately they do not have the protocol file for transfer. I am sorry. I hope however with the tips I gave you last time, you were able to optimize the Western blot. I am sorry to repeat myself, however the transfer conditions are indeed dependent on the equipment used.

The laboratory also recommend to transfer over night and to use 0.01% SDS in the transfer buffer.

Please let me know how you are proceeding. I would be pleased to discuss the optimization further with you.

If you really want to know transfer conditions, you still could contact authors of the publications or Abreviews we do have listed on our website for this antibody.

I am looking forward to hear back from you. Please do not hesitate to contact me also again, should you have any other questions or concerns.

Abcam Scientific Support

Answered on Jul 03 2012

Question

Abcam community

Verified customer

Asked on Jun 29 2012

Answer

Thank you for your answers. I have written to the laboratory to see whether they have the protocol which they have had used. I will get back to you as soon as I have a reply.

I would like however to re-iterate that the efficiency of the blotting depends on the material you are using and is not dependent on the antibody. The western blot transfer should be optimized in your lab according to your conditions and results, as they might differ from laboratory to laboratory.

I can also strongly recommend to perform ponceau red stainings. This is a very fast and reliable method to check the transfer efficiency.

For high molecular weight proteins, I can recommend the following:

- I would highly suggest to optimize the transfer conditions by increasing the transfer time to 18 or 24 hours (in some examples, for big proteins, the transfer is done for 48 hours)

- A transfer at 20V for a long period of time is fine but it has been shown that a "boost" at 70-80V for 1 hour at the end of the transfer can be of significant improvement.

-I do not know the exact composition of your buffer, however you can use up to 0.05%SDS in the transfer buffer and reduce the proportion of methanol to 10%. The reason is that methanol washes out the SDS from the protein.

I hope this information is helpful. Please let me know if you have any question.

Abcam Scientific Support

Answered on Jun 29 2012

1-10 of 23 Abreviews or Q&A

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Key features and details

製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

ChIP Related Products

Compatible Secondaries

Conjugation kits

Isotype control

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

ドメイン

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (53)

レビューと Q&A

Western blot abreview for Anti-NFAT1 antibody [25A10.D6.D2]

Immunohistochemistry (Frozen sections) abreview for Anti-NFAT1 antibody [25A10.D6.D2]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NFAT1 antibody [25A10.D6.D2] - ChIP Grade

ChIP abreview for Anti-NFAT1 antibody [25A10.D6.D2] - ChIP Grade

Western blot abreview for Anti-NFAT1 antibody [25A10.D6.D2]