Anti-Nfasc186 抗体 - Neuronal Marker (ab31719)
Key features and details
- Rabbit polyclonal to Nfasc186 - Neuronal Marker
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Nfasc186 antibody - Neuronal Marker -
製品の詳細
Rabbit polyclonal to Nfasc186 - Neuronal Marker -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat
交差が予測される動物種: Mouse, Human
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 950 - 1050 of Rat Nfasc186.
Immunogen の所有権に関して (Peptide available as ab32637.) -
ポジティブ･コントロール
- Brain (Rat) Whole Tissue Lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab31719の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 186 kDa (predicted molecular weight: 186 kDa).
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| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 186 kDa (predicted molecular weight: 186 kDa). |
ターゲット情報
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関連性
Nfasc186 (Neurofascin 186) is one of two alternatively spliced products of the Neurofascin (Nfasc) gene in rat; the other being Nfasc155. The establishment of paranodal axoglial junctions in myelinated nerves where voltage-gated sodium channels are concentrated at the nodes of Ranvier is determined by myelin-forming glia. The two isoforms of Neurofascin, Nfasc186 in neurons and Nfasc155 in glia, are required for the assembly of these specialized domains. These two major Neurofascins play essential roles in assembling the nodal and paranodal domains of myelinated axons and are essential for the transition to saltatory conduction in developing vertebrate nerves. Nfasc186 (Isoform 1) is expressed at the Nodes of Ranvier while the Nfasc155 (isoforms 2 and 3) are expressed in unmyelinated axons. -
細胞内局在
Cell Membrane; Type I membrane protein. -
参照データベース
- Entrez Gene: 23114 Human
- Entrez Gene: 269116 Mouse
- Entrez Gene: 116690 Rat
- SwissProt: O94856 Human
- SwissProt: Q810U3 Mouse
- SwissProt: P97685 Rat
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別名
- Neurofascin antibody
- NF antibody
- NF186 antibody
see all
画像
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Western blot - Anti-Nfasc186 antibody - Neuronal Marker (ab31719)Anti-Nfasc186 antibody - Neuronal Marker (ab31719) at 1 µg/ml + Brain (Rat) Whole Tissue Lysate at 20 µg
Secondary
IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 186 kDa
Observed band size: 186 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab31719 は 4 報の論文で使用されています。
- Feng T et al. Protective effects of edaravone on white matter pathology in a novel mouse model of Alzheimer's disease with chronic cerebral hypoperfusion. J Cereb Blood Flow Metab 41:1437-1448 (2021). PubMed: 33106078
- Montani L et al. De novo fatty acid synthesis by Schwann cells is essential for peripheral nervous system myelination. J Cell Biol 217:1353-1368 (2018). PubMed: 29434029
- Martin PM et al. Schwannomin-interacting Protein 1 Isoform IQCJ-SCHIP1 Is a Multipartner Ankyrin- and Spectrin-binding Protein Involved in the Organization of Nodes of Ranvier. J Biol Chem 292:2441-2456 (2017). PubMed: 27979964
- Brügger V et al. HDAC1/2-Dependent P0 Expression Maintains Paranodal and Nodal Integrity Independently of Myelin Stability through Interactions with Neurofascins. PLoS Biol 13:e1002258 (2015). PubMed: 26406915