AB2478

Anti-NF2 / Merlin (phospho S518) 抗体

Name: Anti-NF2 / Merlin (phospho S518) 抗体 (ab2478) | Abcam
Brand: Abcam Limited
SKU: AB2478
Price: 74000 JPY
Availability: InStock
Rating: 4 (1 reviews)

Anti-NF2 / Merlin (phospho S518) antibody

BOND RX™ Validated
詳細を見る

(1 Review)

(6 Publications)

Rabbit Polyclonal NF2 / Merlin phospho S518 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human NF2 pS518 aa 500-550 conjugated to Keyhole Limpet Haemocyanin.

別名を表示する

SCH, NF2, Merlin, Moesin-ezrin-radixin-like protein, Neurofibromin-2, Schwannomerlin, Schwannomin

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF2 / Merlin (phospho S518) antibody (AB2478)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF2 / Merlin (phospho S518) antibody (AB2478)

IHC image of ab2478 staining in human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2478, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Unknown

Western blot - Anti-NF2 / Merlin (phospho S518) antibody (AB2478)

Rabbit polyclonal to phospho NF2 (Merlin) Ser 518 (ab2478) was used at a 1/1000 dilution to detect NF2 by Western blot.

Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lane 1) or CLA treated at 4nM, 20 nM or 100 nM (lanes 2,3 and 4 respectively) for 45'. After washing, a 1/5,000 dilution of HRP conjugated Goat-anti-Rabbit IgG (ab7090) was used as secondary antibody.

Rabbit polyclonal to phospho NF2 (Merlin) Ser 518 (ab2478) was used at a 1/1000 dilution to detect NF2 by Western blot.

Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lane 1) or CLA treated at 4nM, 20 nM or 100 nM (lanes 2,3 and 4 respectively) for 45'. After washing, a 1/5,000 dilution of HRP conjugated Goat-anti-Rabbit IgG (ab7090) was used as secondary antibody.

All lanes:

Western blot - Anti-NF2 / Merlin (phospho S518) antibody (ab2478)

Predicted band size: 70 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

WB, IHC-P

applications

免疫原

Synthetic Peptide within Human NF2 pS518 aa 500-550 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P35240

特異性

This affinity purified antibody is directed against human NF2 (Neurofibromatosis 2 gene product). This phospho-specific polyclonal antibody is specific for phosphorylated pS518 of human NF2 (neurofibromatosis 2 gene product).Â Reactivity with non-phosphorylated Human NF2 (Merlin) is minimal by ELISA. Cross-reactivity with NF2 (Merlin) occurs in mouse tissue. Â Reactivity with NF2 from other sources has not been determined.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

This antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.

バッファー組成

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The NF2 gene produces a protein known as Neurofibromin 2 often called Merlin. The NF2 protein or Merlin protein plays a mechanical role in the cell as a tumor suppressor by stabilizing cellular structure and regulating cell growth. Its molar mass is approximately 70 kDa. NF2 protein is found mainly in the nervous system particularly in Schwann cells which are important for nerve function.

Biological function summary

The NF2 protein functions to control cell proliferation differentiation and motility. It associates with the cytoskeleton acting as a bridge between cell surface proteins and the actin cytoskeleton. Merlin as part of the ERM (ezrin radixin moesin) family contributes to the assembly of protein complexes at the cell membrane. Through its interactions it plays a significant role in maintaining proper cell signaling and structural integrity.

Pathways

NF2 protein integrates into the Hippo signaling pathway which regulates organ size by controlling cell growth and apoptosis. Within this pathway Merlin interacts with members such as YAP1 and LATS1/2 moderating their activity to prevent over-proliferation of cells. NF2 also connects with the mTOR pathway which is critical for cell metabolism and growth further emphasizing its regulatory functions in cellular homeostasis.

Mutations or deletions in the NF2 gene are associated with neurofibromatosis type 2 a condition that causes benign tumors on nerve tissues known as schwannomas. NF2 protein is linked to other proteins such as angiogenesis factors contributing to the development of these tumors. Schwannomatosis another disorder related to NF2 involves nerve sheath tumor formation with Merlin mutation playing a role indicating its importance in nervous system health.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.

See full target information NF2 pS518

style

left-column

文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Matrix biology : journal of the International Society for Matrix Biology 116:67-84 PubMed36758905

2023

Low molecular weight hyaluronan inhibits lung epithelial ion channels by activating the calcium-sensing receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed Lazrak,Weifeng Song,Zhihong Yu,Shaoyan Zhang,Anoma Nellore,Charles W Hoopes,Bradford A Woodworth,Sadis Matalon

Science advances 8:eabn2611 PubMed35687678

2022

Gatekeeping role of /Merlin in vascular tip EC induction through suppression of VEGFR2 internalization.

Applications

Unspecified application

Species

Unspecified reactive species

Jung Hyun Bae,Myung Jin Yang,Seung-Hwan Jeong,JungMo Kim,Seon Pyo Hong,Jin Woo Kim,Yoo Hyung Kim,Gou Young Koh

JCI insight 3: PubMed30333319

2018

Influenza-mediated reduction of lung epithelial ion channel activity leads to dysregulated pulmonary fluid homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffrey D Brand,Ahmed Lazrak,John E Trombley,Ren-Jay Shei,A Timothy Adewale,Jennifer L Tipper,Zhihong Yu,Amit R Ashtekar,Steven M Rowe,Sadis Matalon,Kevin S Harrod

Data in brief 15:600-605 PubMed29085875

2017

Data on the regulation of moesin and merlin by the urokinase receptor (uPAR): Model explaining distal activation of integrins by uPAR.

Applications

Unspecified application

Species

Unspecified reactive species

Bernard Degryse,Mishan Britto,Chun Xu Shan,Robert G Wallace,Keith D Rochfort,Philip M Cummins,Gerardene Meade,Ronan P Murphy

Oncogenesis 5:e269 PubMed27841855

2016

Notch3 inhibits epithelial-mesenchymal transition by activating Kibra-mediated Hippo/YAP signaling in breast cancer epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

X Zhang,X Liu,J Luo,W Xiao,X Ye,M Chen,Y Li,G-J Zhang

The Journal of neuroscience : the official journal of the Society for Neuroscience 32:17251-61 PubMed23197717

2012

Rac1 controls Schwann cell myelination through cAMP and NF2/merlin.

Applications

Unspecified application

Species

Unspecified reactive species

Li Guo,Chandra Moon,Karen Niehaus,Yi Zheng,Nancy Ratner

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NF2 / Merlin (phospho S518) 抗体

Anti-NF2 / Merlin (phospho S518) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF2 / Merlin (phospho S518) antibody (AB2478)

Western blot - Anti-NF2 / Merlin (phospho S518) antibody (AB2478)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (6)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise