AB76311

Anti-NF-kB p65 抗体 [EP2161Y]

Name: Anti-NF-kB p65 抗体 [EP2161Y] (ab76311) | Abcam
Brand: Abcam Limited
SKU: AB76311
Price: 29000 JPY
Availability: InStock
Rating: 4 (1 reviews)

Anti-NF-kB p65 antibody [EP2161Y]

20ul selling size
RabMAb
Recombinant
KO Validated
詳細を見る

(1 Review)

(30 Publications)

Rabbit Recombinant Monoclonal NF-kB p65 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 30 publications.

別名を表示する

NFKB3, RELA, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [EP2161Y] (AB76311)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [EP2161Y] (AB76311)

Immunocytochemistry/Immunofluorescence analysis HT-29 (human colorectal adenocarcinoma) labelling NF-kB p65 with purified ab76311 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (ab150077). Nuclei counterstained with DAPI (blue).

Control : PBS only

Flow Cytometry (Intracellular) - Anti-NF-kB p65 antibody [EP2161Y] (AB76311)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-NF-kB p65 antibody [EP2161Y] (AB76311)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling NF-kB p65 with purified ab76311 at 1/1000 dilution (1 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-NF-kB p65 antibody [EP2161Y] (AB76311)

Flow Cytometry analysis of HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labeling NF-kB p65 with purified ab76311 at 1/1000 dilution (1 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Lab

Western blot - Anti-NF-kB p65 antibody [EP2161Y] (AB76311)

Western blot : Anti-NF-kB p65 antibody [EP2161Y] ab76311 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 60 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in RELA knockout MCF7 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-NF-kB p65 antibody [EP2161Y] (ab76311) at 1/1000 dilution

Lane 1:

Wild-type MCF7 cell lysates at 20 µg

Lane 2:

Western blot - Human RELA knockout MCF7 cell line (ab287740) at 40 µg

Lane 3:

PANC-1 cell lysates at 20 µg

Lane 4:

Saos-2 cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 60 kDa

Observed band size: 60 kDa

false

Unknown

Western blot - Anti-NF-kB p65 antibody [EP2161Y] (AB76311)

All lanes:

Western blot - Anti-NF-kB p65 antibody [EP2161Y] (ab76311) at 1/20000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Daudi cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 60 kDa

Observed band size: 70 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP2161Y

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ICC/IF, Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

ab76311 detects both phosphorylated and non-phosphorylated versions of human NF-kB p65.

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Reactivity data

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製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NF-kB p65 also known as RelA is a significant component of the NF-kB protein complex. This complex usually involves a molecular weight for p65 of approximately 65 kDa. NF-kB p65 is mechanistically a transcription factor that regulates genes involved in inflammation cell survival and immune response. Expression of p65 is widely seen in various cell types including immune cells and epithelial cells suggesting its role in numerous physiological processes.

Biological function summary

NF-kB p65 acts as part of the larger NF-kB complex usually forming a heterodimer with other family members like p50. This complex translocates to the nucleus upon activation where it binds specific DNA sequences to regulate gene expression. The p65 subunit is essential for transactivating target genes involved in immune and inflammatory responses. Its regulation is important for proper cellular functioning especially in the maintenance of immune homeostasis and inflammatory response.

Pathways

NF-kB p65 participates in critical pathways like the NF-kB signaling and Toll-like receptor pathways. These pathways are fundamental for initiating immune responses and contribute to the regulation of apoptosis and cellular stress responses. In the context of these pathways the IKK complex plays a pivotal role in NF-kB activation leading to the phosphorylation and subsequent degradation of inhibitors that retain NF-kB p65 in the cytoplasm therefore allowing its movement to the nucleus.

NF-kB p65 is implicated in conditions such as cancer and autoimmune diseases. Its dysregulation can lead to persistent activation of inflammatory pathways contributing to tumorigenesis and chronic inflammation. In the context of these diseases proteins such as the p50 subunit and the IKK complex are often involved reflecting the importance of tight regulation of NF-kB signaling in preventing pathological conditions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric RELA-NFKB1 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. The NF-kappa-B heterodimeric RELA-NFKB1 and RELA-REL complexes, for instance, function as transcriptional activators. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The inhibitory effect of I-kappa-B on NF-kappa-B through retention in the cytoplasm is exerted primarily through the interaction with RELA. RELA shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Besides its activity as a direct transcriptional activator, it is also able to modulate promoters accessibility to transcription factors and thereby indirectly regulate gene expression. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed : 15790681). The NF-kappa-B homodimeric RELA-RELA complex appears to be involved in invasin-mediated activation of IL-8 expression. Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed : 33440148).

See full target information RELA

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文献 (30)

Recent publications for all applications. Explore the full list and refine your search

Journal of dental sciences 20:1579-1589 PubMed40654486

2025

A novel NUTM2A-AS1/miR-769-5p axis regulates LPS-evoked damage in human dental pulp cells via the TLR4/MYD88/NF-κB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Li,Zeru Wang

Allergologia et immunopathologia 53:112-122 PubMed39786883

2025

Acanthoside B attenuates NLRP3-mediated pyroptosis and ulcerative colitis through inhibition of tAGE/RAGE pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaobo He,Chunfang Zhou,Rui Shang,Xiaoyan Wang

Toxics 12: PubMed39195700

2024

Green Tea Polyphenol Nanoparticles Reduce Anxiety Caused by Tobacco Smoking Withdrawal in Rats by Suppressing Neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Alaa M Hammad,Lujain F Alzaghari,Malek Alfaraj,Vanessa Lux,Suhair Sunoqrot

Open medicine (Warsaw, Poland) 19:20240991 PubMed39091610

2024

SHP-1 mediates cigarette smoke extract-induced epithelial-mesenchymal transformation and inflammation in 16HBE cells.

Applications

Unspecified application

Species

Unspecified reactive species

Quan He,Shuanglan Xu,Xiaomei Ma,Yuanxia Qian,Xuzhi Lu,Weiqi Feng,Zi Chen

Scientific reports 14:17429 PubMed39075107

2024

Platelet-derived exosomes regulate endothelial cell inflammation and M1 macrophage polarization in coronary artery thrombosis via modulating miR-34a-5p expression.

Applications

Unspecified application

Species

Unspecified reactive species

Kangkang Wei,Lintong Yu,Jinming Li,Jie Gao,Li Chen,Min Liu,Xiaohan Zhao,Min Li,Dazhuo Shi,Xiaojuan Ma

Molecules (Basel, Switzerland) 28: PubMed37446793

2023

Chronotoxicity of Acrylamide in Mice Fed a High-Fat Diet: The Involvement of Liver CYP2E1 Upregulation and Gut Leakage.

Applications

Unspecified application

Species

Unspecified reactive species

Luanfeng Wang,Yanhong Liu,Huajing Gao,Shuqi Ge,Xinru Yao,Chang Liu,Xintong Tan

Frontiers in pharmacology 13:1047236 PubMed36699078

2023

Acetylsalicylic acid reduces cigarette smoke withdrawal-induced anxiety in rats modulating the expression of NFĸB, GLT-1, and xCT.

Applications

Unspecified application

Species

Unspecified reactive species

Alaa M Hammad,Lujain F Alzaghari,Malek Alfaraj,Walid Al-Qerem,Wamidh H Talib,Fawaz Alasmari,Haneen Amawi,F Scott Hall

International journal of molecular medicine 50: PubMed35762318

2022

Inhibitory effect of PDE2 on inflammation and apoptosis in cerebral ischemia‑reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ding Wan,Xiaoyan Wang,Jin Feng,Peng Wang,Hua Xu

Cell cycle (Georgetown, Tex.) 21:1599-1618 PubMed35404759

2022

Long noncoding RNA BMPR1B-AS1 facilitates endometrial cancer cell proliferation and metastasis by sponging miR-7-2-3p to modulate the DCLK1/Akt/NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tianjiao Lai,Haifeng Qiu,Lulu Si,Yu Zhen,Danxia Chu,Ruixia Guo

Folia neuropathologica 59:335-349 PubMed35114774

2022

Remote ischaemic perconditioning reduces the infarct volume and improves the neurological function of acute ischaemic stroke partially through the miR-153-5p/TLR4/p65/IkBa signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zha,Wei Miao,Wei Rong,Aimei Wang,Weiqi Jiang,Rui Liu,Lanqi Liu,Ying Wang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NF-kB p65 抗体 [EP2161Y]

Anti-NF-kB p65 antibody [EP2161Y]

Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [EP2161Y] (AB76311)

Flow Cytometry (Intracellular) - Anti-NF-kB p65 antibody [EP2161Y] (AB76311)

Flow Cytometry (Intracellular) - Anti-NF-kB p65 antibody [EP2161Y] (AB76311)

Western blot - Anti-NF-kB p65 antibody [EP2161Y] (AB76311)

Western blot - Anti-NF-kB p65 antibody [EP2161Y] (AB76311)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

代替製品

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組合せ製品

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Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (30)

Abcam product promise