Anti-Neutrophil 抗体 [NIMP-R14] (ab2557)
![Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)](https://www.abcam.co.jp/ps/products/2/ab2557/Images/ab2557-282294-anti-neutrophil-antibody-nimp-r14-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)")
Key features and details
- Rat monoclonal [NIMP-R14] to Neutrophil
- Suitable for: IHC-Fr
- Reacts with: Mouse
- Isotype: IgG2b
製品の概要
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製品名
Anti-Neutrophil antibody [NIMP-R14]
Neutrophil 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [NIMP-R14] to Neutrophil -
由来種
Rat -
アプリケーション
適用あり: IHC-Frmore details -
種交差性
交差種: Mouse -
免疫原
Tissue, cells or virus corresponding to Mouse Neutrophil. Purified BALB/c mouse neutrophils
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エピトープ
The monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C. -
特記事項
The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly-6G is together with Ly-6C a component of the myeloid differentiation antigen Gr-1. Ly-6G is a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly-6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading... -
精製度
Protein G purified -
一次抗体 備考
The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly6G is together with Ly6c a component of the myeloid differentiation antigen Gr-1. Ly6G a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days. -
ポリ/モノ
モノクローナル -
クローン名
NIMP-R14 -
ミエローマ
210RCY3-Ag123 -
アイソタイプ
IgG2b -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2557の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr | (4) |
Use at an assay dependent concentration.
Tested with acetone-fixed tissue. |
| 特記事項 |
|---|
|
IHC-Fr
Use at an assay dependent concentration. Tested with acetone-fixed tissue. |
ターゲット情報
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細胞内局在
Cell membrane; Lipid-anchor, GPI-anchor -
参照データベース
- Entrez Gene: 110453 Mouse
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別名
- Ly-6B antibody
- Ly6b antibody
- Lymphocyte antigen 6B antibody
- Lymphocyte antigen 6B Short name=Ly-6B antibody
画像
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Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)
Frozen sections of mouse spleen. ab2557 was used in a concentration of 5 μg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (244)
ab2557 は 244 報の論文で使用されています。
- Faraoni EY et al. Radiofrequency Ablation Remodels the Tumor Microenvironment and Promotes Neutrophil-Mediated Abscopal Immunomodulation in Pancreatic Cancer. Cancer Immunol Res 11:4-12 (2023). PubMed: 36367967
- Ryu JS et al. Inflammation Confers Healing Advantage to Corneal Epithelium Following Subsequent Injury. Int J Mol Sci 24:N/A (2023). PubMed: 36834749
- Wang M et al. TLR4 Blockade Using Docosahexaenoic Acid Restores Vulnerability of Drug-Tolerant Tumor Cells and Prevents Breast Cancer Metastasis and Postsurgical Relapse. ACS Bio Med Chem Au 3:97-113 (2023). PubMed: 37101603
- Ko JH et al. Interferon-γ elicits the ocular surface pathology mimicking dry eye through direct modulation of resident corneal cells. Cell Death Discov 9:209 (2023). PubMed: 37391421
- Kim YB et al. Humulus japonicus ameliorates irritant contact dermatitis by suppressing NF‑κB p65‑dependent inflammatory responses in mice. Exp Ther Med 26:446 (2023). PubMed: 37614435