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AB314079

Anti-Neurofilament-L 抗体 [BLR224K] - BSA free

Anti-Neurofilament-L antibody [BLR224K] - BSA free

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Rabbit Recombinant Monoclonal 68kDa Neurofilament/NF-L antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human, Rat, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human NEFL aa 150-200.

別名を表示する

NF68, NFL, NEFL, Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein

7 Images
Immunocytochemistry - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)
  • ICC

Supplier Data

Immunocytochemistry - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)

Sample : FFPE section of SK-N-BE(2) cells. Antibody : Rabbit anti-Neurofilament-L recombinant monoclonal antibody [BLR224K] (A700-224CF). Secondary : HRP-conjugated goat anti-rabbit IgG.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)

Sample : FFPE section of human medulla. Antibody : Rabbit anti-Neurofilament-L recombinant monoclonal antibody [BLR224K] (A700-224CF). Secondary : HRP-conjugated goat antirabbit IgG.

Immunoprecipitation - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)
  • IP

Supplier Data

Immunoprecipitation - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)

Detection of human Neurofilament-L by western blot of immunoprecipitates. Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies : Rabbit anti-Neurofilament-L recombinant monoclonal antibody [BLR221K] (A700-224) used for IP at 20 µl/mg lysate. Neurofilament-L was also immunoprecipitated by a second antibody against a different epitope of Neurofilament-L (BL-2033B-1C7). For blotting immunoprecipitated Neurofilament-L, A700-224 was used at 1 : 1000. Detection : Chemiluminescence with an exposure time of 30 seconds.

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)

Sample : FFPE section of mouse cerebellum. Antibody : Rabbit anti-Neurofilament-L recombinant monoclonal antibody [BLR224K] (A700-224CF). Secondary : HRP-conjugated goat antirabbit IgG.

Immunocytochemistry - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)
  • ICC

Supplier Data

Immunocytochemistry - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)

Sample : FFPE section of B35 cells. Antibody : Rabbit anti-Neurofilament-L recombinant monoclonal antibody [BLR224K] (A700-224). Secondary : HRP-conjugated goat anti-rabbit IgG.

Western blot - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)
  • WB

Supplier Data

Western blot - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)

Samples : Whole cell lysate (25 µg) from HEK293T, HeLa, SK-N-BE(2), Jurkat, and 786-O cells prepared using NETN lysis buffer. Antibody : Rabbit anti-Neurofilament-L recombinant monoclonal antibody [BLR224K] (A700-224) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Detection : Chemiluminescence with an exposure time of 10 seconds. Lower Panel : Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].

All lanes:

Western blot - Anti-Neurofilament-L antibody [BLR224K] - BSA free (ab314079) at 1/1000 dilution

Lane 1:

HEK293T Whole cell lysate at 25 µg

Lane 2:

HeLa Whole cell lysate at 25 µg

Lane 3:

SK-N-BE(2) Whole cell lysate at 25 µg

Lane 4:

Jurkat Whole cell lysate at 25 µg

Lane 5:

786-O Whole cell lysate at 25 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Western blot - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)
  • WB

Supplier Data

Western blot - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)

Samples : Whole cell lysate (50 µg) from NIH 3T3, CT26, B35, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-Neurofilament-L recombinant monoclonal antibody [BLR224K] (A700-224CF) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Detection : Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Western blot - Anti-Neurofilament-L antibody [BLR224K] - BSA free (ab314079) at 1/1000 dilution

Lane 1:

NIH 3T3 Whole cell lysate at 50 µg

Lane 2:

CT26 Whole cell lysate at 50 µg

Lane 3:

B35 Whole cell lysate at 50 µg

Lane 4:

TCMK-1 Whole cell lysate at 50 µg

Lane 5:

BW5147.3 Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

BLR224K

アイソタイプ

IgG

キャリアフリー

No

交差種

Human, Rat, Mouse

アプリケーション

IP, WB, ICC, IHC-P

applications

免疫原

Synthetic Peptide within Human NEFL aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

P07196

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組合せ製品

Assay Kits

AB288171

Human NEFL ELISA Kit

Recombinant|SimpleStep

ELISA - Human NEFL ELISA Kit (AB288171)

  • 5
  • 1
Primary Antibodies

AB207176

Anti-Neurofilament heavy polypeptide antibody [EPR20020]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (AB207176)

  • 0
  • 0
Primary Antibodies

AB278054

Anti-GFAP antibody [RM1003] - Astrocyte Marker

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [RM1003] - Astrocyte Marker (AB278054)

  • 0
  • 0
Primary Antibodies

AB78078

Anti-beta III Tubulin antibody [2G10] - Neuronal Marker

BOND RX™ Validated|KO Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB78078)

  • 5
  • 41
Primary Antibodies

AB75810

Anti-GAP43 antibody [EP890Y] - Neuronal Marker

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Flow Cytometry (Intracellular) - Anti-GAP43 antibody [EP890Y] - Neuronal Marker (AB75810)

  • 5
  • 8
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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製品の詳細

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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出荷温度及び保存条件

製品の状態
Liquid
バッファー組成
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline
出荷温度
Blue Ice
短期保存期間
Up to 12 months
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The 68kDa Neurofilament also known as NF-L or neurofilament light plays a mechanical role in maintaining the structural integrity of neurons. This protein is characterized by its mass of 68 kilodaltons. It expresses specifically in the cytoplasm of neurons where it forms a critical part of the neurofilament protein triplet along with NF-M and NF-H. These components collectively form the intermediate filaments found in the neuron axons providing essential structural support.
Biological function summary

The neurofilament protein functions to stabilize the axonal diameter thereby affecting neuronal conductivity. It integrates into a complex with other neurofilament proteins and related molecules forming cross-bridges that aid in strengthening the cellular structure. This protein-protein interaction is vital for normal neuronal function and efficient nerve signal transmission.

Pathways

Neurofilament proteins contribute to cellular processes within the nervous system. It participates in the axonal transport pathway which includes the transport of synaptic vesicles and organelles along axons. This movement is facilitated by interactions with motor proteins like kinesin and dynein which help in moving these materials to their destinations. Additionally NF-L connects to actin filaments collaborating to sustain neuronal shape and function.

Neurofilament proteins have links to conditions such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Elevated levels of NF-L are often observed in ALS suggesting neuronal damage as axonal injury raises the protein's presence in the cerebrospinal fluid. Similarly in Alzheimer's disease aberrant interaction of tau protein with neurofilament proteins disrupts neuronal function. These connections highlight NF-L's potential as a biomarker for neural injury and neurodegenerative diseases.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
See full target information NEFL
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Abcam product promise

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