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AB270560

Anti-Neurofilament heavy polypeptide 抗体 [RT97] - BSA and Azide free

Anti-Neurofilament heavy polypeptide antibody [RT97] - BSA and Azide free

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Anti-Neurofilament heavy polypeptide antibody [RT97] - BSA and Azide free (ab270560) is a mouse monoclonal antibody in a PBS only buffer for easy conjugation detecting Neurofilament heavy polypeptide in Western Blot, IHC-P. Suitable for Human, Rat.

- BSA, sodium azide, and glycerol-free for easy conjugation

別名を表示する

KIAA0845, NFH, NEFH, Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [RT97] - BSA and Azide free (AB270560)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [RT97] - BSA and Azide free (AB270560)

Formalin-fixed, paraffin-embedded rat cerebellum tissue stained for Neurofilament heavy polypeptide using ab195503 at 2μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab195503).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [RT97] - BSA and Azide free (AB270560)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [RT97] - BSA and Azide free (AB270560)

Formalin-fixed, paraffin-embedded human cerebellum tissue stained for Neurofilament heavy polypeptide using ab195503 at 2 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab195503).

Western blot - Anti-Neurofilament heavy polypeptide antibody [RT97] - BSA and Azide free (AB270560)
  • WB

Supplier Data

Western blot - Anti-Neurofilament heavy polypeptide antibody [RT97] - BSA and Azide free (AB270560)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab195503).

All lanes:

Anti-Neurofilament heavy polypeptide antibody [RT97] (ab195503) at 2 µg/mL

All lanes:

Human brain tissue lysate

Predicted band size: 112 kDa

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

RT97

アイソタイプ

IgG1

軽鎖のタイプ

kappa

キャリアフリー

Yes

交差種

Rat, Human

アプリケーション

WB, IHC-P

applications

免疫原

Native Full Length Protein corresponding to Human NEFH.

P12036

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組合せ製品

Assay Kits

AB272199

Human Neurofilament H ELISA Kit (200 kDa Neurofilament protein)

Recombinant|SimpleStep

Sandwich ELISA - Human Neurofilament H ELISA Kit (200 kDa Neurofilament protein) (AB272199)

  • 0
  • 0
Primary Antibodies

AB207305

Anti-SQSTM1 / p62 antibody [EPR18351] - Autophagosome Marker

20ul selling size|KO Validated|RabMAb|Recombinant|Lab Essentials|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody [EPR18351] - Autophagosome Marker (AB207305)

  • 5
  • 3
Primary Antibodies

AB223343

Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [EPR22035-112] (AB223343)

  • 0
  • 0
Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

  • 5
  • 76
Primary Antibodies

AB278054

Anti-GFAP antibody [RM1003] - Astrocyte Marker

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [RM1003] - Astrocyte Marker (AB278054)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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製品の詳細

What is this antibody validated in?
Anti-Neurofilament heavy polypeptide antibody [RT97] - BSA and Azide free (ab270560) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Rat samples.

What is the molecular weight of Neurofilament heavy polypeptide?
Anti-Neurofilament heavy polypeptide [RT97] - BSA and Azide free (ab270560) specifically detects a band for Neurofilament heavy polypeptide (UniProt: P12036) at a molecular weight of 112kDa.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A/G
精製に関する特記事項
Purified from bioreactor concentrate.
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Neurofilament heavy polypeptide often referred to as NF-H or neurofilament heavy chain plays an important role in the structural integrity of neurons. This protein is a critical component of the cytoskeleton in neurons consisting of an approximate molecular mass of 200 kDa. It is predominantly expressed in the neuronal axons where it contributes to the assembly and stability of neurofilament structures. NF-H is essential for maintaining axonal diameter and for proper conduction of electrical impulses along nerve fibers.
Biological function summary

This polypeptide participates in the formation of neurofilament a complex structure that includes other neurofilament proteins like the neurofilament light (NF-L) and neurofilament medium (NF-M). These proteins form a stable scaffold within the axon aiding in neuronal growth and repair. The phosphorylation of NF-H modulates its interactions within the neurofilament network influencing axonal transport processes and dynamics which are vital for neuron function and survival.

Pathways

Neurofilament heavy polypeptide is integral to the axonal transport pathway important in conveying molecules between the neuronal cell body and the synaptic terminals. Through its interactions with microtubules and motor proteins NF-H facilitates the movement of organelles and neurotransmitters. It has significant associations with proteins involved in the ubiquitin-proteasome system reflecting its role in maintaining neuronal homeostasis and proteostasis essential in neuronal development and function.

Neurofilament heavy polypeptide serves as an important biomarker for neurodegenerative disorders such as Amyotrophic Lateral Sclerosis (ALS) and Alzheimer's disease. NF-H especially in phosphorylated form accumulates in conditions of axonal injury or degeneration. It often interacts with tau protein in Alzheimer's pathology forming neurofibrillary tangles. Elevated levels of NF-H in cerebrospinal fluid or blood can indicate neuronal damage offering insights into disease progression and potential targets for therapeutic intervention.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
See full target information NEFH
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