Anti-Neurofilament heavy polypeptide 抗体 [RNF402] (ab3966)
![Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [RNF402] (ab3966)](https://www.abcam.co.jp/ps/products/3/ab3966/Images/ab3966-8436-anti-neurofilament-heavy-polypeptide-antibody-rnf402-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [RNF402] (ab3966)")
Key features and details
- Mouse monoclonal [RNF402] to Neurofilament heavy polypeptide
- Suitable for: IHC-Fr
- Reacts with: Rat
- Isotype: IgM
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Neurofilament heavy polypeptide antibody [RNF402]
Neurofilament heavy polypeptide 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [RNF402] to Neurofilament heavy polypeptide -
由来種
Mouse -
特異性
Axonal Marker. This antibody reacts with the intermediate filament protein of central and periferal nerve cells. In Western blotting this antibody is reactive with the Neurofilament heavy polypeptide protein only. This antibody reacts with both the phosphorylated and non-phosphorylated isoforms. -
アプリケーション
適用あり: IHC-Frmore details -
種交差性
交差種: Rat -
免疫原
Tissue, cells or virus corresponding to Cow Neurofilament heavy polypeptide. Neurofilament preparation of calf brain tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
モノクローナル -
クローン名
RNF402 -
ミエローマ
Sp2/0-Ag14 -
アイソタイプ
IgM -
研究分野
関連製品
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Compatible Secondaries
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3966の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr | (1) |
1/10 - 1/100.
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| 特記事項 |
|---|
|
IHC-Fr
1/10 - 1/100. |
ターゲット情報
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機能
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. -
関連疾患
Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. - Information by UniProt
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参照データベース
- Entrez Gene: 24587 Rat
- SwissProt: P16884 Rat
- Unigene: 108194 Rat
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別名
- 200 kDa neurofilament protein antibody
- CMT2CC antibody
- Nefh antibody
see all
画像
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Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [RNF402] (ab3966)
Immunohistochemistry on frozen section of rat brain labeling Neurofilament heavy polypeptide with ab3966.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab3966 は 9 報の論文で使用されています。
- Gao T et al. Interleukin-10 genetically modified clinical-grade mesenchymal stromal cells markedly reinforced functional recovery after spinal cord injury via directing alternative activation of macrophages. Cell Mol Biol Lett 27:27 (2022). PubMed: 35300585
- Liu S et al. Three-dimensional bioprinting sodium alginate/gelatin scaffold combined with neural stem cells and oligodendrocytes markedly promoting nerve regeneration after spinal cord injury. Regen Biomater 9:rbac038 (2022). PubMed: 35801010
- Liu S et al. A multi-channel collagen scaffold loaded with neural stem cells for the repair of spinal cord injury. Neural Regen Res 16:2284-2292 (2021). PubMed: 33818514
- Kim J et al. Nerve Repair and Orthodromic and Antidromic Nerve Grafts: An Experimental Comparative Study in Rabbit. Biomed Res Int 2020:5046832 (2020). PubMed: 31998792
- Liu L et al. Effects of oxygen generating scaffolds on cell survival and functional recovery following acute spinal cord injury in rats. J Mater Sci Mater Med 31:115 (2020). PubMed: 33247423
- Han S et al. Human placenta-derived mesenchymal stem cells loaded on linear ordered collagen scaffold improves functional recovery after completely transected spinal cord injury in canine. Sci China Life Sci 61:2-13 (2018). PubMed: 28527111
- Xiao Z et al. One-year clinical study of NeuroRegen scaffold implantation following scar resection in complete chronic spinal cord injury patients. Sci China Life Sci 59:647-55 (2016). PubMed: 27333785
- Han S et al. The collagen scaffold with collagen binding BDNF enhances functional recovery by facilitating peripheral nerve infiltrating and ingrowth in canine complete spinal cord transection. Spinal Cord 52:867-73 (2014). PubMed: 25311846
- Haro H et al. Experimental chemonucleolysis with recombinant human matrix metalloproteinase 7 in human herniated discs and dogs. Spine J N/A:N/A (2013). Human . PubMed: 24295797