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Anti-Neurofilament heavy polypeptide 抗体 [NF-01] (ab7795)

Reviews (5)Q&A (2)References (27)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)
  • Flow Cytometry - Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)

Key features and details

  • Mouse monoclonal [NF-01] to Neurofilament heavy polypeptide
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Neurofilament heavy polypeptide antibody [NF-01]
    Neurofilament heavy polypeptide 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [NF-01] to Neurofilament heavy polypeptide

  • 由来種

    Mouse

  • 特異性

    This antibody recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa) of various species.

  • アプリケーション

    適用あり: IHC-P, Flow Cytmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: a wide range of other species, Mammals

  • 免疫原

    Full length native protein (purified) corresponding to Pig Neurofilament heavy polypeptide. A pellet of pig brain cold-stable proteins after depolymerization of microtubules.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab7795の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (1)
1/5 - 1/10.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
特記事項
IHC-P
1/5 - 1/10.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.

  • 関連疾患

    Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.

  • 配列類似性

    Belongs to the intermediate filament family.

  • 翻訳後修飾

    There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
    Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 200 kDa neurofilament protein antibody
      • CMT2CC antibody
      • Nefh antibody
      • Neurofilament heavy polypeptide 200kDa antibody
      • Neurofilament heavy polypeptide antibody
      • Neurofilament triplet H protein antibody
      • NF H antibody
      • NF-H antibody
      • NFH antibody
      • NFH_HUMAN antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)

      Immunohistochemical analysis of paraffin-embedded human cerebellum tissue sections labelling Neurofilament heavy polypeptide protein with ab7795 at 5 µL.

    • Flow Cytometry - Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)
      Flow Cytometry - Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)

      Overlay histogram showing SH-SY5Y cells stained with ab7795 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7795, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488  (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    参考文献 (27)

    ab7795 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab7795 は 27 報の論文で使用されています。

    • Awad H  et al. Endovascular repair and open repair surgery of thoraco-abdominal aortic aneurysms cause drastically different types of spinal cord injury. Sci Rep 11:7834 (2021). PubMed: 33837260
    • Song S  et al. Electrical stimulation of human neural stem cells via conductive polymer nerve guides enhances peripheral nerve recovery. Biomaterials 275:120982 (2021). PubMed: 34214785
    • Liang L  et al. Melatonin pretreatment alleviates the long-term synaptic toxicity and dysmyelination induced by neonatal Sevoflurane exposure via MT1 receptor-mediated Wnt signaling modulation. J Pineal Res 71:e12771 (2021). PubMed: 34585785
    • Alabduljabbar M  et al. The clinico-pathologic profile of primary and recurrent orbital/periorbital plexiform neurofibromas (OPPN). PLoS One 16:e0258802 (2021). PubMed: 34673814
    • Ronchi S  et al. Electrophysiological Phenotype Characterization of Human iPSC-Derived Neuronal Cell Lines by Means of High-Density Microelectrode Arrays. Adv Biol (Weinh) 5:e2000223 (2021). PubMed: 33729694
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-7 of 7 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neurofilament heavy polypeptide antibody [NF-01]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Flex TRS High pH
    Specification
    Brain
    Blocking step
    (agent) for 5 minute(s) · Concentration: 0.25% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Mark Butters

    Verified customer

    投稿 Feb 02 2021

    Immunohistochemistry (Frozen sections) abreview for Anti-Neurofilament heavy polypeptide antibody [NF-01]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Horse Tissue sections (Recurrent Laryngeal Nerve)
    Permeabilization
    No
    Specification
    Recurrent Laryngeal Nerve
    Fixative
    Frozen in Isopentane and Liquid Nitrogen
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Stephen Cahalan

    Verified customer

    投稿 May 10 2017

    Immunocytochemistry abreview for Anti-Neurofilament heavy polypeptide antibody [NF-01]

     Average
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Mouse Cell (mESC)
    Permeabilization
    Yes - tritonx100
    Specification
    mESC
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Kyungjoo Seong

    Verified customer

    投稿 Nov 02 2016

    IHC - Wholemount abreview for Anti-200 kD Neurofilament Heavy antibody [NF-01] - Neuronal Marker

     Good
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Rat Tissue (Dorsal root ganglion)
    Specification
    Dorsal root ganglion
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Shlomo Tsuriel

    Verified customer

    投稿 Feb 06 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-200 kD Neurofilament Heavy antibody [NF-01] - Neuronal Marker

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Differentiated embryonal carcinoma cells)
    Specification
    Differentiated embryonal carcinoma cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton-x-100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Mar 18 2009

    Question

    To whom correspond,

    Dear Madame/Sir,

    we used frequently the antibody mouse SMI-32 to bind to Non-phosphorylated Neurofilament Heavy Chain (old reference in Abcam: ab-73273-250, 330E per vial). We have noticed you do not sold this refecence anymore. Could you suggest another antibody reference to replace that one?

    Thank you very much in advance.

    Kind regards,
    --

    Read More
    Answer

    Thank you for your inquiry.

    I can confirm that we do not have ab73273 in our catalog anymore.

    I can suggest ab7795 as alternative. It also recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa) of various species.

    https://www.abcam.com/Neurofilament-heavy-polypeptide-antibody-NF-01-ab7795.html (or use the following: https://www.abcam.com/Neurofilament-heavy-polypeptide-antibody-NF-01-ab7795.html).

    I hope this information is helpful and wish you good luck with your research.

    Read More

    Question

    We intend to order this antibody providing it works in mouse tissues. Can you guarantee this? if not, can you please suggest an alternative? Thank you very much.

    Read More
    Answer

    Please refer the paper on the datasheet (Histochemistry 100, 495,1993) which features the use of this monoclonal antibody. "In similar preparations (the authors refer to pellet of brain proteins after cold depolymerization, described elsewhere in the text) isolated from mouse and rat brains, the antibody also reacted with the protein at the NF-H position." The paper follows expression of phosphorylated NF-H in human developing dorsal root ganglia and spinal cord and makes no other note about reactivity of ab7795 with mouse tissue sections. To summarise, we are pretty sure that the antibody cross reacts with mouse.

    Read More

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