Anti-Neurofilament heavy polypeptide 抗体

• IHC-FrFl

Supplier Data

Immunohistochemistry - Free Floating - Anti-Neurofilament heavy polypeptide antibody (AB4680)

Immunohistological analysis of a rat cerebellum section stained with ab4680 at a dilution 1 : 5,000 in red, and co-stained with rabbit pAb to GFAP at a dilution 1 : 5,000 in green. The blue is DAPI staining of nuclear DNA.

Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 μM, and free floating sections were stained.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody (AB4680)

Human neuroblastoma SH-SY5Y cells stained with chicken anti-NF-H (red) and mouse monoclonal to fibrillarin 38F3 (green). Nuclear DNA is revealed with Hoechst dye (blue). The NF-H antibody was used at a dilution of 1/100000 and the fibrillarin monoclonal at 1/1000. Cultures were processed using our standard fixation and staining procedure (in protocol section).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody (AB4680)

Rat Neurons stained with chicken anti-NF-H (green). The NF-H antibody was used at a dilution of 1/100000 using our standard fixation and staining procedure (in protocol section).

• ICC

PubMed

Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (AB4680)

ab4680 staining Neurofilament heavy polypeptide (green) in murine embryoid bodies by Immunocytochemistry/ Immunofluorescence. Nuclei were visualized with DAPI.

Image from Wirt SE et al, J Cell Biol. 2010 Nov 15;191(4):809-25. Epub 2010 Nov 8, Fig 4. DOI 10.1083/jcb.201003048

• ICC

Supplier Data

Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (AB4680)

Mixed neuron/glial cultures stained with a mouse monoclonal antibody to neurofilament subunit NF-L (green) and ab4680, chicken antibody to neurofilament heavy polypeptide. This antibody binds primarily to the phosphorylated axonal forms of NF-H, in contrast to the NF-L antibody which stains both axonal and dendritic/perikaryal neurofilaments. The NF-L antibody therefore reveals a prominent cell body in green, while the surrounding axonal profiles are orange, since the are bound by both NF-L and the chicken NF-H antibody. Blue is a DNA stain.

• WB

Supplier Data

Western blot - Anti-Neurofilament heavy polypeptide antibody (AB4680)

All lanes:

Western blot - Anti-Neurofilament heavy polypeptide antibody (ab4680) at 1/20000 dilution

Lane 1:

Rat spinal cord lysate

Lane 2:

Mouse spinal cord lysate

Lane 3:

Cow spinal cord lysate

Predicted band size: 112 kDa

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• WB

CiteAb

Western blot - Anti-Neurofilament heavy polypeptide antibody (AB4680)

Neurofilament heavy polypeptide western blot using anti-Neurofilament heavy polypeptide antibody ab4680. Publication image and figure legend from Dun, X. P., Carr, L., et al., 2019, Cell Rep, PubMed 30726731.

ab4680 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab4680 please see the product overview.

Ectopic Schwann Cell Migration in the Nerve Bridge of Sox2 KO Mice and Sox2 Regulating Robo1 Expression in SCs(A) Schwann cell (GFP+) migration from both proximal and distal nerve stumps in control mice 6 days after sciatic nerve transection injury.(B) Ectopic Schwann cell migration (white arrows) in the nerve bridge of Sox2 KO mice 6 days after transection injury.(C) Higher magnification image from (B, dotted-line square) showing regenerating axons (labeled with neurofilament, red, indicated by arrowheads) following the ectopic migrating Schwann cells (white arrows) and leaving the nerve bridge.(D) Schwann cells stayed in the nerve bridge in control mice at 14 days following sciatic nerve transection injury.(E) Ectopic migrating Schwann cells (white arrows) leaving the nerve bridge in Sox2 KO mice at 14 days after injury.(F) Ectopic migrating Schwann cells (white arrows) localizing in front of regenerating axons (indicated by arrowheads) of Sox2 KO mice.Scale bar in (A, B, D and E) represents 200 μm, in (C) represents 60 μm, and in (F) represents 30 μm.(G and H) Microarray data (G) and RT-PCR (H) from control and Sox2-overexpressing Schwann cells. Values in (G) represent the fluorescence intensity of dye-labeled cDNA fragments that have hybridized to the probes on the microarray chip.(I) qRT-PCR validation of Robo1 mRNA upregulation in Sox2-overexpressing Schwann cells, n = 3.(J) Quantification of Robo1 protein levels in the distal nerve stump of control (Con) and Sox2 KO mice at 4 and 7 days after injury, n = 3.(K) Western blots showing Robo1 and Robo2 protein upregulation in Sox2-overexpressing Schwann cells.(L) Western blots comparing Robo1 and Robo2 protein levels in the distal nerve stump of control and Sox2 KO mice at 4 and 7 days after injury.(M) Western blot comparing Robo1 protein levels in the distal nerve stump of control (Con) and Sox2-overexpressing (OE) mice at 7 days following injury.(N) Quantification of Robo1 protein levels from (M), n = 3.(O) RT-PCR showing that Slit3 is highly expressed in the nerve bridge at 7 days after injury; dorsal root ganglion (DRG) samples have been used as positive controls.** in (I), (J), and (N) indicate p < 0.01 compared with controls.Several z series were captured on a Zeiss LSM510 confocal microscope in (A), (B), (D), and (E), covering the entire field of interest. The individual series were then flattened into a single image for each location and combined into one image using Adobe Photoshop software (Adobe Systems).

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