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リコンビナントRabMAb

Anti-Neurofascin 抗体 [EPR19003] (ab184377)

Submit a review Submit a question References (1)
Western blot - Anti-Neurofascin antibody [EPR19003] (ab184377)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] (ab184377)
  • Western blot - Anti-Neurofascin antibody [EPR19003] (ab184377)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] (ab184377)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] (ab184377)
  • Anti-Neurofascin antibody [EPR19003] (ab184377)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19003] to Neurofascin
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Neurofascin antibody [EPR19003]
    Neurofascin 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR19003] to Neurofascin

  • 由来種

    Rabbit

  • 特異性

    IHC is recommended for mouse and rat only.

  • アプリケーション

    適用あり: IHC-P, WBmore details
    適用なし: ICC

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human brain and cerebellum lysates; Mouse and rat brain and spinal cord lysates. IHC-P: Mouse cerebrum and colon tissues. Rat cerebrum tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184377の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/2000. Detects a band of approximately 186, 155, 125, 75 kDa (predicted molecular weight: 138 kDa).
特記事項
IHC-P
1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/2000. Detects a band of approximately 186, 155, 125, 75 kDa (predicted molecular weight: 138 kDa).
追加情報
Is unsuitable for ICC.

ターゲット情報

画像

  • Western blot - Anti-Neurofascin antibody [EPR19003] (ab184377)
    Western blot - Anti-Neurofascin antibody [EPR19003] (ab184377)
    All lanes : Anti-Neurofascin antibody [EPR19003] (ab184377) at 1/2000 dilution

    Lane 1 : Human brain lysate at 20 µg
    Lane 2 : Human cerebellum lysate at 20 µg
    Lane 3 : Human lung lysate at 20 µg
    Lane 4 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 138 kDa
    Observed band size: 125,155,186,75 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight and expression profile observed are consistent with the literatures (PMID: 23160985; PMID: 9562181).

    The 75 kDa band represents one of the isoforms annotated in UniProt.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] (ab184377)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] (ab184377)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Neurofascin with ab184377 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasm staining on mouse cerebrum is observed [PMID: 23198679].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Neurofascin antibody [EPR19003] (ab184377)
    Western blot - Anti-Neurofascin antibody [EPR19003] (ab184377)
    All lanes : Anti-Neurofascin antibody [EPR19003] (ab184377) at 1/2000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : Mouse spinal cord lysate
    Lane 4 : Rat spinal cord lysate
    Lane 5 : Mouse lung lysate
    Lane 6 : Mouse heart lysate
    Lane 7 : Mouse kidney lysate
    Lane 8 : Rat kidney lysate
    Lane 9 : Rat spleen lysate
    Lane 10 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 11 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 12 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 138 kDa
    Observed band size: 125,155,186,75 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight and expression profile observed are consistent with the literatures (PMID: 23160985; PMID: 9562181).

    The 75 kDa band represents one of the isoforms annotated in UniProt.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] (ab184377)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] (ab184377)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Neurofascin with ab184377 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasm and membrane staining on myenteric nerve plexus, and negative on epithelium cells and smooth muscle cells of mouse colon.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] (ab184377)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] (ab184377)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Neurofascin with ab184377 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasm staining on rat cerebrum is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Neurofascin antibody [EPR19003] (ab184377)
    Anti-Neurofascin antibody [EPR19003] (ab184377)

参考文献 (1)

ab184377 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab184377 は 1 報の論文で使用されています。

  • Miguel-Hidalgo JJ  et al. Reduced length of nodes of Ranvier and altered proteoglycan immunoreactivity in prefrontal white matter in major depressive disorder and chronically stressed rats. Sci Rep 13:16419 (2023). PubMed: 37775676

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