Anti-Neurofascin 抗体 (ab31457)
Key features and details
- Rabbit polyclonal to Neurofascin
- Suitable for: WB, ICC/IF, IHC-FoFr, IHC-P
- Reacts with: Mouse, Rat, Human, Common marmoset
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Neurofascin antibody
Neurofascin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Neurofascin -
由来種
Rabbit -
特異性
The ab31457 antibody would be able to bind to the full length canonical sequence Isoform 1 and also to Isoforms 5, Isoform 6, Isoform 9 and Isoform 13.
The antibody would not be able to detect Isoforms 2, 3, 4, 7, 8, 10, 11 or Isoform 12. All of these Isoforms are missing amino acids 1035-1203. -
アプリケーション
適用あり: WB, ICC/IF, IHC-FoFr, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human, Common marmoset -
免疫原
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab31457の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 155,186 kDa (predicted molecular weight: 138 kDa).
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| IHC-FoFr | (1) |
1/1000.
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| IHC-P | (3) |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 155,186 kDa (predicted molecular weight: 138 kDa). |
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ICC/IF
Use a concentration of 1 µg/ml. |
|
IHC-FoFr
1/1000. |
|
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Cell adhesion, ankyrin-binding protein which may be involved in neurite extension, axonal guidance, synaptogenesis, myelination and neuron-glial cell interactions. -
配列類似性
Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.
Contains 5 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 23114 Human
- Entrez Gene: 269116 Mouse
- Entrez Gene: 116690 Rat
- Omim: 609145 Human
- SwissProt: O94856 Human
- SwissProt: Q810U3 Mouse
- SwissProt: P97685 Rat
- Unigene: 13349 Human
see all -
別名
- KIAA0756 antibody
- Neurofascin antibody
- Neurofascin homolog antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Neurofascin antibody (ab31457)ab31457 staining Neurofascin in Rat Primary Neurons DIV14 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab31457 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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Western blot - Anti-Neurofascin antibody (ab31457)Anti-Neurofascin antibody (ab31457) at 1 µg/ml + Brain(Mouse)Whole Cell Lysate at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 138 kDa
Observed band size: 155,186 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa, 55 kDa. We are unsure as to the identity of these extra bands.In Rat, Neurofascin has three observed isoforms , isoform 1 (138 kDa) which is known as Nfasc186, isoform 2 (133 kDa) and isoform 3 (132 kDa) which are both known as Nfasc155. These proteins are highly glycosylated and their observed molecular weight in Western blot is 186 kDa and 155 kDa respectively.
Mouse Neurofascin has a predicted molecular weight of 138 kDa (Swiss-Prot data) however the observed bands at 186 kDa and 155 kDa are thought to correspond to the glycosylated isoforms 1, 2 and 3 as observed in Rat (Sherman DL et al, 2005,PubMed:16337912) -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Neurofascin antibody (ab31457)This image is courtesy of Sophie Pezet, CNRS, Paris, FranceImmunohistochemistical detection of Neurofascin using antibody ab31457 on PFA perfusion-fixed rat brain sections. Primary antibody ab31457 was used at 1/1000 incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: goat anti-rabbit Alexa Fluor 488 conjugated (1/1000). Immunostaining obtained in the rat hippocampus using this antibody diluted 1:1000. Sections come from a perfused-fixed animal. The immunostaining was performed using the `free floating` technique, using direct fluorescence. Some cytoplasmic staining was observed in granular neurons and neuronal processes (arrows on the figure on the right). The lefthand figure shows the hippocampal granular cell staining (objective X20), the righthand figure shows this at higher magnification. This staining is consistent with published observation of cytoplasmic localisation of Neurofascin in the cytoplasm of hippocampal cells and their axon initial segment (Burkarth
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (24)
ab31457 は 24 報の論文で使用されています。
- Stajković N et al. Direct fluorescent labeling of NF186 and NaV1.6 in living primary neurons using bioorthogonal click chemistry. J Cell Sci 136:N/A (2023). PubMed: 37288813
- Wang Y et al. Glucose increases the length and spacing of the lattice structure of the axon initial segment. Microsc Res Tech 85:2679-2691 (2022). PubMed: 35411984
- Chen P et al. Identification of NFASC and CHL1 as Two Novel Hub Genes in Endometriosis Using Integrated Bioinformatic Analysis and Experimental Verification. Pharmgenomics Pers Med 15:377-392 (2022). PubMed: 35496348
- He L et al. Crystal structure of Ankyrin-G in complex with a fragment of Neurofascin reveals binding mechanisms required for integrity of the axon initial segment. J Biol Chem 298:102272 (2022). PubMed: 35850303
- Mazuir E et al. Oligodendrocyte Secreted Factors Shape Hippocampal GABAergic Neuron Transcriptome and Physiology. Cereb Cortex 31:5024-5041 (2021). PubMed: 34023893