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AB109406

Anti-NeuroD2 抗体 [EPR5135]

Anti-NeuroD2 antibody [EPR5135]

5

(3 Reviews)

|

(8 Publications)

Rabbit Recombinant Monoclonal NeuroD2 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 8 publications.

別名を表示する

BHLHA1, NDRF, NEUROD2, Neurogenic differentiation factor 2, NeuroD2, Class A basic helix-loop-helix protein 1, NeuroD-related factor, bHLHa1

3 Images
Western blot - Anti-NeuroD2 antibody [EPR5135] (AB109406)
  • WB

Unknown

Western blot - Anti-NeuroD2 antibody [EPR5135] (AB109406)

All lanes:

Western blot - Anti-NeuroD2 antibody [EPR5135] (ab109406) at 1/1000 dilution

Lane 1:

Human cerebellum lysate at 10 µg

Lane 2:

Human hippocampus lysate at 10 µg

Lane 3:

Human fetal brain lysates at 10 µg

Predicted band size: 41 kDa

Observed band size: 48 kDa

false

Western blot - Anti-NeuroD2 antibody [EPR5135] (AB109406)
  • WB

Lab

Western blot - Anti-NeuroD2 antibody [EPR5135] (AB109406)

All lanes:

Western blot - Anti-NeuroD2 antibody [EPR5135] (ab109406) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

Human fetal brain tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 41 kDa

Observed band size: 48 kDa

false

Western blot - Anti-NeuroD2 antibody [EPR5135] (AB109406)
  • WB

Lab

Western blot - Anti-NeuroD2 antibody [EPR5135] (AB109406)

All lanes:

Western blot - Anti-NeuroD2 antibody [EPR5135] (ab109406) at 1/1000 dilution

Lane 1:

Mouse cerebellum lysates at 15 µg

Lane 2:

Rat cerebellum lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 41 kDa

Observed band size: 48 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-NeuroD2 antibody [EPR5135] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR5135

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat, Human

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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製品の詳細

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Stable for 12 months at -20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NeuroD2 also known as Neuronal Differentiation 2 is a transcription factor involved in neurogenesis. It weighs approximately 40 kDa. This protein is part of the basic helix-loop-helix (bHLH) family which regulates gene expression. NeuroD2 is expressed mainly in the nervous system particularly in the developing brain and certain adult brain regions. Its expression pattern suggests an important role in neural development and maintenance.
Biological function summary

NeuroD2 influences neuronal differentiation and survival. It guides precursor cells toward a neuronal fate influencing neurogenesis and impacting brain structure. NeuroD2 does not form large protein complexes but interacts closely with other bHLH transcription factors. These interactions help regulate the transcription of genes necessary for the development and maturation of neurons.

Pathways

Transcription factors such as NeuroD2 play key roles in the neurogenesis pathway and the calcium signaling pathway. It operates in tandem with other proteins like NeuroD1 to effectively guide neural precursor cells toward differentiation into mature neurons. Through these pathways NeuroD2 helps regulate genes that are important for brain development and function.

Abnormal NeuroD2 expression relates to neurological conditions like autism spectrum disorders and certain types of epilepsy. Mutations or dysregulation in NeuroD2 can affect associated proteins like REST (RE1-Silencing Transcription factor) which plays a role in neuronal gene silencing. Understanding these connections provides insights into how NeuroD2 influences neural-related diseases and offers potential targets for therapeutic intervention.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Transcriptional regulator implicated in neuronal determination. Mediates calcium-dependent transcription activation by binding to E box-containing promoter. Critical factor essential for the repression of the genetic program for neuronal differentiation; prevents the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. Induces transcription of ZEB1, which in turn represses neuronal differentiation by down-regulating REST expression. Plays a role in the establishment and maturation of thalamocortical connections; involved in the segregation of thalamic afferents into distinct barrel domains within layer VI of the somatosensory cortex. Involved in the development of the cerebellar and hippocampal granular neurons, neurons in the basolateral nucleus of amygdala and the hypothalamic-pituitary axis. Associates with chromatin to the DPYSL3 E box-containing promoter (By similarity).
See full target information NEUROD2
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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 11:2588 PubMed32444594

2020

KAT3-dependent acetylation of cell type-specific genes maintains neuronal identity in the adult mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Michal Lipinski,Rafael Muñoz-Viana,Beatriz Del Blanco,Angel Marquez-Galera,Juan Medrano-Relinque,José M Caramés,Andrzej A Szczepankiewicz,Jordi Fernandez-Albert,Carmen M Navarrón,Roman Olivares,Grzegorz M Wilczyński,Santiago Canals,Jose P Lopez-Atalaya,Angel Barco

Scientific reports 9:19697 PubMed31873146

2019

Terminal neuron localization to the upper cortical plate is controlled by the transcription factor NEUROD2.

Applications

Unspecified application

Species

Unspecified reactive species

Gizem Guzelsoy,Cansu Akkaya,Dila Atak,Cory D Dunn,Alkan Kabakcioglu,Nurhan Ozlu,Gulayse Ince-Dunn

Science advances 5:eaax0080 PubMed31681843

2019

Reprogramming of DNA methylation at NEUROD2-bound sequences during cortical neuron differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Maria A Hahn,Seung-Gi Jin,Arthur X Li,Jiancheng Liu,Zhijun Huang,Xiwei Wu,Byung-Wook Kim,Jennifer Johnson,Adrienne-Denise V Bilbao,Shu Tao,Jacob A Yim,Yuman Fong,Sandra Goebbels,Markus H Schwab,Qiang Lu,Gerd P Pfeifer

Nature communications 10:4112 PubMed31511512

2019

In vivo epigenetic editing of Sema6a promoter reverses transcallosal dysconnectivity caused by C11orf46/Arl14ep risk gene.

Applications

Unspecified application

Species

Unspecified reactive species

Cyril J Peter,Atsushi Saito,Yuto Hasegawa,Yuya Tanaka,Mohika Nagpal,Gabriel Perez,Emily Alway,Sergio Espeso-Gil,Tariq Fayyad,Chana Ratner,Aslihan Dincer,Achla Gupta,Lakshmi Devi,John G Pappas,François M Lalonde,John A Butman,Joan C Han,Schahram Akbarian,Atsushi Kamiya

International journal of molecular sciences 20: PubMed30934833

2019

Mechanistic Insights in NeuroD Potentiation of Mineralocorticoid Receptor Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa T C M van Weert,Jacobus C Buurstede,Hetty C M Sips,Isabel M Mol,Tanvi Puri,Ruth Damsteegt,Benno Roozendaal,R Angela Sarabdjitsingh,Onno C Meijer

eNeuro 4: PubMed28303257

2017

NEUROD2 Regulates Expression and Store-Operated Calcium Entry in Cortical Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Gokhan Guner,Gizem Guzelsoy,Fatma Sadife Isleyen,Gulcan Semra Sahin,Cansu Akkaya,Efil Bayam,Eser Ilgin Kotan,Alkan Kabakcioglu,Gulayse Ince-Dunn

BMC genomics 16:681 PubMed26341353

2015

Genome-wide target analysis of NEUROD2 provides new insights into regulation of cortical projection neuron migration and differentiation.

Applications

Unspecified application

Species

Mouse

Efil Bayam,Gulcan Semra Sahin,Gizem Guzelsoy,Gokhan Guner,Alkan Kabakcioglu,Gulayse Ince-Dunn

The Journal of biological chemistry 287:27396-406 PubMed22736761

2012

Contribution of microRNA-1275 to Claudin11 protein suppression via a polycomb-mediated silencing mechanism in human glioma stem-like cells.

Applications

WB

Species

Human

Keisuke Katsushima,Keiko Shinjo,Atsushi Natsume,Fumiharu Ohka,Makiko Fujii,Hirotaka Osada,Yoshitaka Sekido,Yutaka Kondo
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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関連製品

Select an associated product type
Alternative Product
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5

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