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リコンビナントRabMAb

Anti-NeuroD1 抗体 [EPR17084] (ab205300)

Submit a review Submit a question References (8)
Immunohistochemistry (Frozen sections) - Anti-NeuroD1 antibody [EPR17084] (ab205300)
  • Western blot - Anti-NeuroD1 antibody [EPR17084] (ab205300)
  • Immunocytochemistry/ Immunofluorescence - Anti-NeuroD1 antibody [EPR17084] (ab205300)
  • Immunohistochemistry (Frozen sections) - Anti-NeuroD1 antibody [EPR17084] (ab205300)
  • Immunocytochemistry/ Immunofluorescence - Anti-NeuroD1 antibody [EPR17084] (ab205300)
  • Immunocytochemistry/ Immunofluorescence - Anti-NeuroD1 antibody [EPR17084] (ab205300)
  • Western blot - Anti-NeuroD1 antibody [EPR17084] (ab205300)
  • Immunohistochemistry (Frozen sections) - Anti-NeuroD1 antibody [EPR17084] (ab205300)
  • Immunohistochemistry (Frozen sections) - Anti-NeuroD1 antibody [EPR17084] (ab205300)
  • Anti-NeuroD1 antibody [EPR17084] (ab205300)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17084] to NeuroD1
  • Suitable for: WB, ICC/IF, IHC-Fr
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free

製品の概要

  • 製品名

    Anti-NeuroD1 antibody [EPR17084]
    NeuroD1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR17084] to NeuroD1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IF, IHC-Frmore details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human cerebellum lysate; Y79 whole cell lysate. ICC/IF: SH-SY5Y, Y79 and mouse primary neural/glia cells. IHC-Fr: Frozen mouse brain tissue sections and mouse hippocampus.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab205300の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 40 kDa).
ICC/IF
1/100 - 1/250.
IHC-Fr
Use a concentration of 10 µg/ml.
特記事項
WB
1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 40 kDa).
ICC/IF
1/100 - 1/250.
IHC-Fr
Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能

    Differentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex. Transcriptional activator. Binds to the insulin gene E-box.

  • 関連疾患

    Defects in NEUROD1 are the cause of maturity-onset diabetes of the young type 6 (MODY6) [MIM:606394]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.

  • 配列類似性

    Contains 1 basic helix-loop-helix (bHLH) domain.

  • 翻訳後修飾

    Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth.

  • 細胞内局在

    Cytoplasm. Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • atonal antibody
      • basic helix loop helix transcription factor antibody
      • BETA 2 antibody
      • Beta cell E box transactivator 2 antibody
      • BETA2 antibody
      • BHF 1 antibody
      • BHF1 antibody
      • bHLHa3 antibody
      • class A basic helix loop helix protein 3 antibody
      • Class A basic helix-loop-helix protein 3 antibody
      • MODY 6 antibody
      • MODY6 antibody
      • NDF1_HUMAN antibody
      • NeuroD antibody
      • NeuroD1 antibody
      • Neurogenic differentiation 1 antibody
      • Neurogenic differentiation factor 1 antibody
      • neurogenic helix loop helix protein NEUROD antibody
      • Neuronal differentiation 1 antibody
      see all

    画像

    • Immunohistochemistry (Frozen sections) - Anti-NeuroD1 antibody [EPR17084] (ab205300)
      Immunohistochemistry (Frozen sections) - Anti-NeuroD1 antibody [EPR17084] (ab205300)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized fresh Mouse hippocampus tissue labelling NeuroD1 with ab205300 at 1/50 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary at 1/1000 dilution. Positive staining on mouse hippocampus tissue is observed. The nuclear counterstain was DAPI (Blue).

    • Western blot - Anti-NeuroD1 antibody [EPR17084] (ab205300)
      Western blot - Anti-NeuroD1 antibody [EPR17084] (ab205300)
      Anti-NeuroD1 antibody [EPR17084] (ab205300) at 1/5000 dilution + Y79 (Human retinoblastoma cell line) whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

      Predicted band size: 40 kDa
      Observed band size: 47 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-NeuroD1 antibody [EPR17084] (ab205300)
      Immunocytochemistry/ Immunofluorescence - Anti-NeuroD1 antibody [EPR17084] (ab205300)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labelling NeuroD1 with primary antibody anti-NeuroD1 (ab205300) at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 1/1000 dilution. Confocal image showing nuclear staining in part of mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Anti-MAP2 mouse monoclonal antibody (ab11267) was used to counterstain at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) as a secondary counterstain antibody at 1/1000 dilution. The nuclear counter stain is DAPI (blue).

      The negative controls are as follows:-

      Negative control 1: ab205300 at 1/100 dilution followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution.
      Negative control 2: ab11267 Anti-MAP2 mouse monoclonal antibody at 1/500 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

    • Immunohistochemistry (Frozen sections) - Anti-NeuroD1 antibody [EPR17084] (ab205300)
      Immunohistochemistry (Frozen sections) - Anti-NeuroD1 antibody [EPR17084] (ab205300)

      IHC image of NeuroD1 staining in a section of frozen normal mouse brain performed on a Leica Biosystems BOND® RX instrument. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab205300, 10 ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Immunocytochemistry/ Immunofluorescence - Anti-NeuroD1 antibody [EPR17084] (ab205300)
      Immunocytochemistry/ Immunofluorescence - Anti-NeuroD1 antibody [EPR17084] (ab205300)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Y79 (Human retinoblastoma cell line) cells labeling NeuroD1 with ab205300 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Confocal image showing cytoplasmic and nuclear staining on Y79 cell line.

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

      The negative controls are as follows:-

      -ve control 1: ab205300 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-NeuroD1 antibody [EPR17084] (ab205300)
      Immunocytochemistry/ Immunofluorescence - Anti-NeuroD1 antibody [EPR17084] (ab205300)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling NeuroD1 with ab205300 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Confocal image showing cytoplasmic and nuclear staining on SH-SY5Y cell line.

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

      The negative controls are as follows:-

      -ve control 1: ab205300 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

    • Western blot - Anti-NeuroD1 antibody [EPR17084] (ab205300)
      Western blot - Anti-NeuroD1 antibody [EPR17084] (ab205300)
      All lanes : Anti-NeuroD1 antibody [EPR17084] (ab205300) at 1/1000 dilution

      Lane 1 : Human cerebellum lysate
      Lane 2 : Human fetal heart lysate
      Lane 3 : Human fetal kidney lysate
      Lane 4 : Human fetal spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 40 kDa
      Observed band size: 47 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Frozen sections) - Anti-NeuroD1 antibody [EPR17084] (ab205300)
      Immunohistochemistry (Frozen sections) - Anti-NeuroD1 antibody [EPR17084] (ab205300)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized fresh Mouse liver tissue labelling NeuroD1 with ab205300 at 1/50 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary at 1/1000 dilution. No staining on mouse liver tissue is observed. The nuclear counterstain was DAPI (Blue). Negative control (PMID: 24309898).

    • Immunohistochemistry (Frozen sections) - Anti-NeuroD1 antibody [EPR17084] (ab205300)
      Immunohistochemistry (Frozen sections) - Anti-NeuroD1 antibody [EPR17084] (ab205300)

      Negative tissue image: IHC image of NeuroD1 staining in a section of frozen normal mouse liver performed on a Leica Biosystems BOND® RX instrument. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab205300, 10 ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Anti-NeuroD1 antibody [EPR17084] (ab205300)
      Anti-NeuroD1 antibody [EPR17084] (ab205300)

    参考文献 (8)

    ab205300 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab205300 は 8 報の論文で使用されています。

    • Denize T  et al. Concordance of ASCL1, NEUROD1 and POU2F3 transcription factor-based subtype assignment in paired tumour samples from small cell lung carcinoma. Histopathology 83:912-924 (2023). PubMed: 37644667
    • Chen P  et al. YAP1 expression is associated with survival and immunosuppression in small cell lung cancer. Cell Death Dis 14:636 (2023). PubMed: 37752152
    • Seo JH & Jeon YJ Global Proteomic Analysis of Mesenchymal Stem Cells Derived from Human Embryonic Stem Cells via Connective Tissue Growth Factor Treatment under Chemically Defined Feeder-Free Culture Conditions. J Microbiol Biotechnol 32:126-140 (2022). PubMed: 34750284
    • Anastasaki C  et al. Generation of human induced pluripotent stem cell-derived cerebral organoids for cellular and molecular characterization. STAR Protoc 3:101173 (2022). PubMed: 35199037
    • Cejas P  et al. Subtype heterogeneity and epigenetic convergence in neuroendocrine prostate cancer. Nat Commun 12:5775 (2021). PubMed: 34599169
    • Chan JM  et al. Signatures of plasticity, metastasis, and immunosuppression in an atlas of human small cell lung cancer. Cancer Cell 39:1479-1496.e18 (2021). PubMed: 34653364
    • Zhong L  et al. HDAC9 Silencing Exerts Neuroprotection Against Ischemic Brain Injury via miR-20a-Dependent Downregulation of NeuroD1. Front Cell Neurosci 14:544285 (2020). PubMed: 33584204
    • Dammert MA  et al. MYC paralog-dependent apoptotic priming orchestrates a spectrum of vulnerabilities in small cell lung cancer. Nat Commun 10:3485 (2019). PubMed: 31375684

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