Anti-Neurocan 抗体 [650.24] (ab31979)
![Immunocytochemistry/ Immunofluorescence - Anti-Neurocan antibody [650.24] (ab31979)](https://www.abcam.co.jp/ps/products/31/ab31979/Images/ab31979-43568-anti-neurocan-antibody-65024-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Neurocan antibody [650.24] (ab31979)")
Key features and details
- Mouse monoclonal [650.24] to Neurocan
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Neurocan antibody [650.24]
Neurocan 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [650.24] to Neurocan -
由来種
Mouse -
特異性
Specific for Neurocan core protein; also reacts with neurocan C fragment -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Tissue, cells or virus corresponding to Rat Neurocan.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
650.24 -
ミエローマ
P3x63 -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab31979の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (4) |
Use at an assay dependent concentration.
|
| WB |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid. -
組織特異性
Brain. -
配列類似性
Belongs to the aggrecan/versican proteoglycan family.
Contains 1 C-type lectin domain.
Contains 2 EGF-like domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Contains 2 Link domains.
Contains 1 Sushi (CCP/SCR) domain. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 1463 Human
- Entrez Gene: 13004 Mouse
- Entrez Gene: 58982 Rat
- Omim: 600826 Human
- SwissProt: O14594 Human
- SwissProt: P55066 Mouse
- SwissProt: P55067 Rat
- Unigene: 169047 Human
see all -
別名
- Chondroitin sulfate proteoglycan 3 antibody
- Cspg3 antibody
- Ncan antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Neurocan antibody [650.24] (ab31979)Image courtesy of an anonymous Abreview.ab31979 staining Neurocan in rat glioma cell line C6 by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab31979 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-mouse polyclonal, used at a 1/300 dilution. Nuclei are counterstained with DAPI.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab31979 は 4 報の論文で使用されています。
- Wei J et al. Spatiotemporal proteomic atlas of multiple brain regions across early fetal to neonatal stages in cynomolgus monkey. Nat Commun 14:3917 (2023). PubMed: 37400444
- Su H et al. TMAO Aggregates Neurological Damage Following Ischemic Stroke by Promoting Reactive Astrocytosis and Glial Scar Formation via the Smurf2/ALK5 Axis. Front Cell Neurosci 15:569424 (2021). PubMed: 33815059
- Huang X et al. Overexpression of the transcription factors OCT4 and KLF4 improves motor function after spinal cord injury. CNS Neurosci Ther N/A:N/A (2020). PubMed: 32449258
- Li G et al. Mdivi-1 Inhibits Astrocyte Activation and Astroglial Scar Formation and Enhances Axonal Regeneration after Spinal Cord Injury in Rats. Front Cell Neurosci 10:241 (2016). WB, ICC/IF ; Rat . PubMed: 27807407