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Anti-Neurexin 1 抗体 (ab77596)

Reviews (2)Q&A (2)References (3)
Western blot - Anti-Neurexin 1 antibody (ab77596)

    Key features and details

    • Goat polyclonal to Neurexin 1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-Neurexin 1 antibody

    • 製品の詳細

      Goat polyclonal to Neurexin 1

    • 由来種

      Goat

    • 特異性

      This antibody will detect Neurexin 1 beta and Neurexin 1 alpha (isoform 1a, 2a and 3a).

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse, Rat, Dog

    • 免疫原

      Synthetic peptide corresponding to Human Neurexin I beta (C terminal). The immunogen sequence is aa419-432 of Neurexin 1 beta (P58400), aa1454-1467 of Neurexin 1 alpha (Q9ULB1), aa1476-1489 of Neurexin 1 alpha (Q9ULB1-2), and aa1524-1537 of Neurexin 1 alpha (Q9ULB1-3).
      Sequence:

      KEKQPSSAKSSNKN

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab77596の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    Use a concentration of 0.05 - 0.1 µg/ml. Predicted molecular weight: 162 kDa.

    A 1 hour primary incubation is recommended for this product.
    特記事項
    WB
    Use a concentration of 0.05 - 0.1 µg/ml. Predicted molecular weight: 162 kDa.

    A 1 hour primary incubation is recommended for this product.

    ターゲット情報

    • 機能

      Cell surface protein involved in cell-cell-interactions, exocytosis of secretory granules and regulation of signal transmission. Function is isoform-specific. Alpha-type isoforms have a long N-terminus with six laminin G-like domains and play an important role in synaptic signal transmission. Alpha-type isoforms play a role in the regulation of calcium channel activity and Ca(2+)-triggered neurotransmitter release at synapses and at neuromuscular junctions. They play an important role in Ca(2+)-triggered exocytosis of secretory granules in pituitary gland. They may effect their functions at synapses and in endocrine cells via their interactions with proteins from the exocytotic machinery. Likewise, alpha-type isoforms play a role in regulating the activity of postsynaptic NMDA receptors, a subtype of glutamate-gated ion channels. Both alpha-type and beta-type isoforms may play a role in the formation or maintenance of synaptic junctions via their calcium-dependent interactions (via the extracellular domains) with neuroligin family members, CBLN1 or CBLN2. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses. Alpha-type isoforms were first identified as receptors for alpha-latrotoxin from spider venom.

    • 組織特異性

      Brain.

    • 配列類似性

      Belongs to the neurexin family.
      Contains 3 EGF-like domains.
      Contains 6 laminin G-like domains.

    • 翻訳後修飾

      N-glycosylated.
      O-glycosylated.

    • 細胞内局在

      Cell membrane. Cell junction, synapse. Localized on the pre-synaptic membrane.
    • Information by UniProt

    • 参照データベース

      see all

    • 製品の状態

      The neurexin genes use alternate promoters, splice sites and exons to encode potentially hundreds or thousands of mRNA transcripts.

    • 別名

      • DKFZp313P2036 antibody
      • FLJ35941 antibody
      • Hs.22998 antibody
      • KIAA0578 antibody
      • Neurexin 1 alpha antibody
      • Neurexin 1 beta antibody
      • Neurexin I-alpha antibody
      • Neurexin-1 antibody
      • Neurexin-1-alpha antibody
      • NRX1A_HUMAN antibody
      • NRXN1 antibody
      • PTHSL2 antibody
      • SCZD17 antibody
      see all

    画像

    • Western blot - Anti-Neurexin 1 antibody (ab77596)
      Western blot - Anti-Neurexin 1 antibody (ab77596)
      Anti-Neurexin 1 antibody (ab77596) at 0.05 µg/ml + human brain (cerebellum) lysate in RIPA buffer at 35 µg

      Developed using the ECL technique.

      Predicted band size: 162 kDa
      Observed band size: 150 kDa why is the actual band size different from the predicted?



      Primary antibody incubation: 1 hour

    参考文献 (3)

    ab77596 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab77596 は 3 報の論文で使用されています。

    • Dore K  et al. SYNPLA, a method to identify synapses displaying plasticity after learning. Proc Natl Acad Sci U S A 117:3214-3219 (2020). PubMed: 31974314
    • Torres-Vega E  et al. Netrin-1 receptor antibodies in thymoma-associated neuromyotonia with myasthenia gravis. Neurology 88:1235-1242 (2017). PubMed: 28251919
    • Wei H  et al. Redox/methylation mediated abnormal DNA methylation as regulators of ambient fine particulate matter-induced neurodevelopment related impairment in human neuronal cells. Sci Rep 6:33402 (2016). WB . PubMed: 27624276

    レビューと Q&A

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    レビューを送る 質問を送る

    1-4 of 4 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Neurexin 1 antibody

     Average
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Midbrian and Striatum assembloids)
    Permeabilization
    Yes - 0.5% Triton in PBS
    Specification
    Midbrian and Striatum assembloids
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Apr 01 2022

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Neurexin 1 antibody

     Poor
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Beta cell)
    Permeabilization
    Yes - 0.1% saponin
    Specification
    Beta cell
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Aug 24 2018

    Question

    The predicted band size of this product is 162 kDa. However, the predicted molecular weight of Neurexin 1beta is 72kD according to the software. I want to know why the molecular weight is different?

    Read More
    Answer



    There seems to be a few different isoforms of Neurexin I beta. For example, P58400 (NRX1B_HUMAN) notes a protein of nearly 47kDa. Another form, Q9ULB1 (NRX1A_HUMAN), is listed as nearly 162kDa, which is the form referred to on the online product datasheet. Here is a link to the Uniprot entry: http://www.uniprot.org/uniprot/Q9ULB1#section_seq.

    Read More

    Question

    Product code: 77596

    Inquiry: Our customer wishes to test ab77596 in ICC/IF with human cells. Are you interested in this type of the information for the Abreview promotion (The customer buys certain antibodies and upload Abreview on Abcam's website and then we give 100% credit note for this antibody to buy next antibody)?
    Thank you in advance. Kind regards,

    Read More
    Answer

    Thank you for your message. I am very pleased to hear the customer would like to make use of our testing discount offer and test ab77596 in ICC/IF.

    DISCOUNT CODE: ####
    Expiration date: ####

    This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ICC-IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    If you have any further questions, please do not hesitate to contact us. We look forward to receiving the Abreview and wish the customer luck with their research.

    Read More

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    For licensing inquiries, please contact partnerships@abcam.com