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AB239347

Anti-NeuN 抗体 [EPR21906] - BSA and Azide free

Anti-NeuN antibody [EPR21906] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal NeuN antibody. Carrier free. Suitable for IP, WB, IHC-Fr, mIHC, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

別名を表示する

RNA binding protein fox-1 homolog 3, Fox-1 homolog C, Neuronal nuclei antigen, NeuN antigen, RBFOX3

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR21906] - BSA and Azide free (AB239347)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR21906] - BSA and Azide free (AB239347)

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling NeuN with ab236870 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in granule cells of human cerebellum (PMID : 8813082; PMID : 1483388). Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab236870).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR21906] - BSA and Azide free (AB239347)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR21906] - BSA and Azide free (AB239347)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling NeuN with ab236870 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in neurons of human cerebrum (PMID : 8813082; PMID : 1483388; PMID : 26085943). Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236870).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-NeuN antibody [EPR21906] - BSA and Azide free (AB239347)
  • IP

Supplier Data

Immunoprecipitation - Anti-NeuN antibody [EPR21906] - BSA and Azide free (AB239347)

NeuN was immunoprecipitated from 0.35 mg human cerebellum lysate with ab236870 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab236870 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Human cerebellum lysate 10 μg (Input).
Lane 2 : ab236870 IP in human cerebellum lysate (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab236870 in human cerebellum lysate (-).

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 30 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID : 1483388; PMID : 26085943).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236870).

All lanes:

Immunoprecipitation - Anti-NeuN antibody [EPR21906] - Neuronal Marker (<a href='/products/primary-antibodies/neun-antibody-epr21906-neuronal-marker-ab236870'>ab236870</a>)

Predicted band size: 34 kDa

false

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR21906] - BSA and Azide free (AB239347)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR21906] - BSA and Azide free (AB239347)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebellum tissue labeling NeuN with ab236870 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic and nuclear staining in mouse cerebellum (PMID : 19713214).

The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236870).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR21906] - BSA and Azide free (AB239347)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR21906] - BSA and Azide free (AB239347)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling NeuN with ab236870 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in neurons of rat cerebrum (PMID : 8813082; PMID : 1483388; PMID : 26085943). Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236870).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Multiplex immunohistochemistry - Anti-NeuN antibody [EPR21906] - BSA and Azide free (AB239347)
  • mIHC

Lab

Multiplex immunohistochemistry - Anti-NeuN antibody [EPR21906] - BSA and Azide free (AB239347)

This data was developed using ab236870, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse hippocampus tissue staining SLC7A5/LAT1 with ab324354 at a 1/2000 dilution, ab236870 anti-NeuN/RBFOX3 used at 1/10000 dilution and ab254264 anti-MAP2 used at a 1/4000 dilution.

Panel A : anti-SLC7A5/LAT1 (green; Opal™520), anti-NeuN/RBFOX3 (magenta; Opal™690) and anti-MAP2 (gray; Opal™570) on mouse cerebrum.

Panel B : anti-SLC7A5/LAT1 staining blood vessels in mouse cerebrum.

Panel C : anti-NeuN/RBFOX3 staining nucleus of neurons in mouse cerebrum.

Panel D : anti-MAP2 staining cell body and dendrites of neurons in mouse cerebrum.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324354, ab236870 and ab254264 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR21906] - BSA and Azide free (AB239347)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR21906] - BSA and Azide free (AB239347)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebellum tissue labeling NeuN with ab236870 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic and nuclear staining in rat cerebellum (PMID : 19713214).

The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236870).

Multiplex immunohistochemistry - Anti-NeuN antibody [EPR21906] - BSA and Azide free (AB239347)
  • mIHC

Lab

Multiplex immunohistochemistry - Anti-NeuN antibody [EPR21906] - BSA and Azide free (AB239347)

This data was developed using ab236870, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse hippocampus tissue staining SLC7A5/LAT1 with ab324354 at a 1/2000 dilution, ab236870 anti-NeuN/RBFOX3 used at 1/10000 dilution and ab254264 anti-MAP2 used at a 1/4000 dilution.

Panel A : anti-SLC7A5/LAT1 (green; Opal™520), anti-NeuN/RBFOX3 (magenta; Opal™690) and anti-MAP2 (gray; Opal™570) on mouse hippocampus.

Panel B : anti-SLC7A5/LAT1 staining blood vessels in mouse hippocampus.

Panel C : anti-NeuN/RBFOX3 staining nucleus of neurons in mouse hippocampus.

Panel D : anti-MAP2 staining cell body and dendrites of neurons in mouse hippocampus.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324354, ab236870 and ab254264 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR21906] - BSA and Azide free (AB239347)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR21906] - BSA and Azide free (AB239347)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling NeuN with ab236870 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in neurons of mouse cerebrum (PMID : 8813082; PMID : 1483388; PMID : 26085943). Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236870).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

関連する標識済み抗体及び組成の異なる製品 (1)

  • Unconjugated

    Anti-NeuN antibody [EPR21906] - Neuronal Marker

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR21906

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Mouse, Rat, Human

アプリケーション

mIHC, WB, IHC-Fr, IP, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "mIHC" : {"fullname" : "Multiplex immunohistochemistry", "shortname":"mIHC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "mIHC-species-checked": "guaranteed", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "", "mIHC-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p>Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).</p>", "mIHC-species-checked": "guaranteed", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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製品の詳細

ab239347 is the carrier-free version of ab236870.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

NeuN also known as Fox-3 or Rbfox3 serves as a vital neuronal marker and has a molecular weight of about 46-50 kDa. This nuclear protein is commonly found in neuronal cells in the central nervous system but it does not appear in glial cells. Researchers use NeuN staining as an effective tool to identify neurons in various brain regions due to its reliable expression pattern in mature neurons. The presence of NeuN can be detected using methods such as NeuN IHC (immunohistochemistry) and NeuN Western blot aiding in cellular and tissue analysis.
Biological function summary

NeuN plays an essential role in the regulation of RNA-binding influencing the splicing and stability of transcripts. It forms part of the complex network responsible for managing neuron-specific gene expression. By interacting with other RNA-binding proteins NeuN contributes to the fine-tuning needed for proper neuronal development and function. As a result NeuN helps maintain the health and activity of nerve cells allowing them to perform complex neurological tasks.

Pathways

NeuN has a significant role in neuronal differentiation and plasticity pathways. NeuN coordinates with elements of the Notch signaling pathway which is important for guiding cell fate decisions in the nervous system. It also interacts with related proteins like Fox proteins involved in splicing regulation. These pathways ensure neurons develop differentiate and function correctly within the nervous system's precise architecture.

Disruptions in NeuN expression link closely to neurodegenerative diseases such as Parkinson's disease and Amyotrophic lateral sclerosis (ALS). Abnormal NeuN expression correlates with neuron loss and dysfunction in these conditions with connectivity to other affected proteins like alpha-synuclein in Parkinson's disease. In ALS abnormal NeuN-staining patterns indicate neuronal cell death serving as a potential marker for disease progression. Studying these connections improves our understanding of disease mechanisms and aids in the development of targeted therapies.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD).
See full target information NeuN
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in physiology 11:441 PubMed32528301

2020

Exosomal MicroRNA-320a Derived From Mesenchymal Stem Cells Regulates Rheumatoid Arthritis Fibroblast-Like Synoviocyte Activation by Suppressing CXCL9 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Meng,Bing Qiu
View all publications
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代替製品

Primary Antibodies

AB207281

Alexa Fluor® 555 Anti-NeuN antibody [EPR12763] - Neuronal Marker

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB207281)

  • 5
  • 2
Primary Antibodies

AB190195

Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB190195)

  • 4
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Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

  • 5
  • 74
Primary Antibodies

AB190565

Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB190565)

  • 5
  • 7
Primary Antibodies

AB207282

Alexa Fluor® 568 Anti-NeuN antibody [EPR12763] - Neuronal Marker

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB207282)

  • 5
  • 1
Primary Antibodies

AB204681

Biotin Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB204681)

  • 0
  • 0
Primary Antibodies

AB207279

Alexa Fluor® 594 Anti-NeuN antibody [EPR12763] - Neuronal Marker

RabMAb|Recombinant

Immunocytochemistry - Alexa Fluor® 594 Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB207279)

  • 5
  • 1

組合せ製品

Primary Antibodies

AB68428

Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB68428)

  • 5
  • 20
Primary Antibodies

AB178846

Anti-Iba1 antibody [EPR16588] - Microglia marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Microglia marker (AB178846)

  • 5
  • 50
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB32454

Anti-MAP2 antibody - Neuronal Marker

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody - Neuronal Marker (AB32454)

  • 5
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Abcam product promise

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