Anti-Netrin-3 抗体 (ab185200)
Key features and details
- Rabbit polyclonal to Netrin-3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Netrin-3 antibody -
製品の詳細
Rabbit polyclonal to Netrin-3 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human Netrin-3 aa 1-150.
Database link: O00634 -
ポジティブ･コントロール
- Human plasma (IgG/HSA depleted)
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185200の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 61 kDa.
|
| 特記事項 |
|---|
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 61 kDa. |
ターゲット情報
-
機能
Netrins control guidance of CNS commissural axons and peripheral motor axons. -
組織特異性
Spinal cord. -
配列類似性
Contains 3 laminin EGF-like domains.
Contains 1 laminin N-terminal domain.
Contains 1 NTR domain. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
-
参照データベース
- Entrez Gene: 4917 Human
- Omim: 602349 Human
- SwissProt: O00634 Human
- Unigene: 158336 Human
-
別名
- NET3_HUMAN antibody
- Netrin-2 antibody
- Netrin-2-like protein antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Netrin-3 antibody (ab185200)
Immunohistochemical analysis of paraffin embedded Human oral mucosa tissue labeling Netrin-3 with ab185200 at 1/50 and counterstained with hematoxylin.
-
Western blot - Anti-Netrin-3 antibody (ab185200)All lanes : Anti-Netrin-3 antibody (ab185200) at 1/250 dilution
Lane 1 : RT 4 cell line lysate
Lane 2 : U 251MG cell line lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Secondary
All lanes : Swine anti-rabbit HRP conjugated antibody at 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 61 kDa
Exposure time: 1 minute
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab185200 は 2 報の論文で使用されています。
- Jiang S et al. Targeting netrin-3 in small cell lung cancer and neuroblastoma. EMBO Mol Med 13:e12878 (2021). PubMed: 33719214
- Dominici C et al. Floor-plate-derived netrin-1 is dispensable for commissural axon guidance. Nature 545:350-354 (2017). PubMed: 28445456