Anti-Nesprin3 抗体 [EPR15623] (ab186746)
![Western blot - Anti-Nesprin3 antibody [EPR15623] (ab186746)](https://www.abcam.co.jp/ps/products/186/ab186746/Images/ab186746-219991-ab1867461.jpg "Western blot - Anti-Nesprin3 antibody [EPR15623] (ab186746)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15623] to Nesprin3
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Nesprin3 antibody [EPR15623]
Nesprin3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR15623] to Nesprin3 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Jurkat and 293 cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR15623 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186746の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 112 kDa.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 112 kDa. |
ターゲット情報
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機能
Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. -
配列類似性
Belongs to the nesprin family.
Contains 1 KASH domain.
Contains 2 spectrin repeats. -
ドメイン
The KASH domain is involved in the binding to SUN1 and SUN2 through recognition of their SUN domains. -
細胞内局在
Nucleus outer membrane. Located at the rough reticulum endoplasmic (RER) in some cells. - Information by UniProt
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参照データベース
- Entrez Gene: 161176 Human
- Omim: 610861 Human
- SwissProt: Q6ZMZ3 Human
- Unigene: 354645 Human
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別名
- C14orf49 antibody
- Chromosome 14 open reading frame 49 antibody
- FLJ25605 antibody
see all
画像
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Western blot - Anti-Nesprin3 antibody [EPR15623] (ab186746)All lanes : Anti-Nesprin3 antibody [EPR15623] (ab186746) at 1/5000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 112 kDaBlocking/ Dilution buffer: 5% NFDM /TBST.
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![Anti-Nesprin3 antibody [EPR15623] (ab186746)](https://www.abcam.co.jp/ps/products/186/ab186746/Images/ab186746-2-benefits-of-recombinant-antibodies.png)
Anti-Nesprin3 antibody [EPR15623] (ab186746)
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab186746 は 1 報の論文で使用されています。
- Zhang C et al. ISGylation of EMD promotes its interaction with PDHA to inhibit aerobic oxidation in lung adenocarcinoma. J Cell Mol Med 26:5078-5094 (2022). PubMed: 36071546