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AB186746

Anti-Nesprin3 抗体 [EPR15623]

Anti-Nesprin3 antibody [EPR15623]

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(2 Publications)

Rabbit Recombinant Monoclonal Nesprin3 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

別名を表示する

C14orf139, C14orf49, LINC00341, SYNE3, Nesprin-3, KASH domain-containing protein 3, Nuclear envelope spectrin repeat protein 3, KASH3

1 Images
Western blot - Anti-Nesprin3 antibody [EPR15623] (AB186746)
  • WB

Supplier Data

Western blot - Anti-Nesprin3 antibody [EPR15623] (AB186746)

Blocking/ Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Nesprin3 antibody [EPR15623] (ab186746) at 1/5000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 112 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-Nesprin3 antibody [EPR15623] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR15623

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Nesprin-3 also known as SYNE3 is a mechanical adaptor protein of around 215 kDa. It belongs to the spectrin repeat family and is primarily expressed in the outer nuclear membrane. This protein anchors the nucleus to the cytoskeleton by interacting with cytoskeletal linkers like plectin. It plays a role in maintaining the structural integrity of the nucleus and cellular architecture due to these anchoring functions.
Biological function summary

Nesprin-3 is involved in the linking of the nuclear envelope to the cytoskeleton and it forms part of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex. This complex is important for nuclear movement within cells and has a role in signal transduction from the cell surface to the nucleus. Through its ability to bind to other cytoskeletal elements Nesprin-3 contributes to force transmission across the cell impacting cell polarity and migration.

Pathways

Nesprin-3 interacts with several cytoskeletal signaling pathways particularly the actin and intermediate filament pathways. It associates with proteins like plectin and vimentin playing significant parts in cellular mechanics and signaling. By engaging in these pathways Nesprin-3 helps control cellular responses to mechanical stress and influences various processes including cell migration and division.

Nesprin-3 has been linked to muscular dystrophies and certain cancers. In muscular dystrophies its interactions with plectin can become disrupted leading to compromised muscle integrity and function. In cancer changes in its expression or interactions may affect cell movement and invasion contributing to metastasis. Its role in these diseases highlights its importance in maintaining proper cell architecture and function.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. Plays a role in the regulation of aortic epithelial cell morphology, and is required for flow-induced centrosome polarization and directional migration in aortic endothelial cells.
See full target information SYNE3
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed39996719

2025

BCL-B Promotes Lung Cancer Invasiveness by Direct Inhibition of BOK.

Applications

Unspecified application

Species

Unspecified reactive species

Palaniappan Ramesh,Amal R Al Kadi,Gaurav M Borse,Maximilian Webendörfer,Gregor Zaun,Martin Metzenmacher,Fabian Doerr,Servet Bölükbas,Balazs Hegedüs,Smiths S Lueong,Joelle Magne,Beiyun Liu,Greisly Nunez,Martin Schuler,Douglas R Green,Halime Kalkavan

Journal of cellular and molecular medicine 26:5078-5094 PubMed36071546

2022

ISGylation of EMD promotes its interaction with PDHA to inhibit aerobic oxidation in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Congcong Zhang,Jiangtao Cui,Leiqun Cao,Xiaoting Tian,Yayou Miao,Yikun Wang,Shiyu Qiu,Wanxin Guo,Lifang Ma,Jinjing Xia,Xiao Zhang
View all publications
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代替製品

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組合せ製品

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