AB74261

Anti-Nesprin3 抗体

Name: Anti-Nesprin3 抗体 (ab74261) | Abcam
Brand: Abcam Limited
SKU: AB74261
Price: 75000 JPY
Availability: InStock

Anti-Nesprin3 antibody

Be the first to review this product! Submit a review

(5 Publications)

Rabbit Polyclonal Nesprin3 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human SYNE3.

別名を表示する

C14orf139, C14orf49, LINC00341, SYNE3, Nesprin-3, KASH domain-containing protein 3, Nuclear envelope spectrin repeat protein 3, KASH3

1 Images

Unknown

Western blot - Anti-Nesprin3 antibody (AB74261)

All lanes:

Western blot - Anti-Nesprin3 antibody (ab74261) at 1/500 dilution

Lane 1:

Extracts from Jurkat cells at 5 µg

Lane 2:

Extracts from Jurkat cells plus 5µg immunizing peptide at 5 µg

Predicted band size: 112 kDa

Observed band size: 112 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

Synthetic Peptide within Human SYNE3. The exact immunogen used to generate this antibody is proprietary information.

Q6ZMZ3

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitopespecific immunogen.

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Nesprin-3 also known as SYNE3 is a mechanical adaptor protein of around 215 kDa. It belongs to the spectrin repeat family and is primarily expressed in the outer nuclear membrane. This protein anchors the nucleus to the cytoskeleton by interacting with cytoskeletal linkers like plectin. It plays a role in maintaining the structural integrity of the nucleus and cellular architecture due to these anchoring functions.

Biological function summary

Nesprin-3 is involved in the linking of the nuclear envelope to the cytoskeleton and it forms part of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex. This complex is important for nuclear movement within cells and has a role in signal transduction from the cell surface to the nucleus. Through its ability to bind to other cytoskeletal elements Nesprin-3 contributes to force transmission across the cell impacting cell polarity and migration.

Pathways

Nesprin-3 interacts with several cytoskeletal signaling pathways particularly the actin and intermediate filament pathways. It associates with proteins like plectin and vimentin playing significant parts in cellular mechanics and signaling. By engaging in these pathways Nesprin-3 helps control cellular responses to mechanical stress and influences various processes including cell migration and division.

Nesprin-3 has been linked to muscular dystrophies and certain cancers. In muscular dystrophies its interactions with plectin can become disrupted leading to compromised muscle integrity and function. In cancer changes in its expression or interactions may affect cell movement and invasion contributing to metastasis. Its role in these diseases highlights its importance in maintaining proper cell architecture and function.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. Plays a role in the regulation of aortic epithelial cell morphology, and is required for flow-induced centrosome polarization and directional migration in aortic endothelial cells.

See full target information SYNE3

style

left-column

文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Science advances 7:eabg6908 PubMed34524844

2021

MYO10 drives genomic instability and inflammation in cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Franklin Mayca Pozo,Xinran Geng,Ilaria Tamagno,Mark W Jackson,Ernest G Heimsath,John A Hammer,Richard E Cheney,Youwei Zhang

Science advances 7: PubMed33523987

2021

The nuclear piston activates mechanosensitive ion channels to generate cell migration paths in confining microenvironments.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Pyo Lee,Farid Alisafaei,Kolade Adebawale,Julie Chang,Vivek B Shenoy,Ovijit Chaudhuri

Cell metabolism 30:903-916.e7 PubMed31523006

2019

Nuclear Glycogenolysis Modulates Histone Acetylation in Human Non-Small Cell Lung Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Ramon C Sun,Vikas V Dukhande,Zhengqiu Zhou,Lyndsay E A Young,Shane Emanuelle,Christine Fillmore Brainson,Matthew S Gentry

Oncology reports 40:783-792 PubMed29901114

2018

Sperm‑associated antigen 4 (SPAG4) as a new cancer marker interacts with Nesprin3 to regulate cell migration in lung carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Ji,Jinquan Jiang,Lihua Huang,Wei Feng,Zhang Zhang,Longyu Jin,Xiaowei Xing

Proceedings of the National Academy of Sciences of 107:5076-81 PubMed20145110

2010

Abnormal development of the cerebral cortex and cerebellum in the setting of lamin B2 deficiency.

Applications

ICC/IF, WB

Species

Mouse, Mouse

Catherine Coffinier,Sandy Y Chang,Chika Nobumori,Yiping Tu,Emily A Farber,Julia I Toth,Loren G Fong,Stephen G Young

View all publications

style

left-column

style

left-column

style

right-column